Exploited Application of Bacillus sp. ETL-A & Pseudomonas sp. ETL-B in Microbial Degradation of Orange 16 Dye

Maulin P Shah^1,

¹Industrial Waste Water Research Laboratory, Division of Applied & Environmental Microbiology, Enviro Technology Limited, GIDC, Ankleshwar, Gujarat, India

Cite this paper:
Maulin P Shah. Exploited Application of Bacillus sp. ETL-A & Pseudomonas sp. ETL-B in Microbial Degradation of Orange 16 Dye. International Journal of Environmental Bioremediation & Biodegradation. 2014; 2(1):37-43. doi: 10.12691/ijebb-2-1-7

Abstract

Soil samples collected from common effluent treatment plant of Ankleshwar were used as a source. Two different bacterial strains are capable of decolorizing Orange 16. The individual bacterial strains Bacillus sp. ETL-A and Pseudomonas sp. ETL-B decolorized Orange 16 (200 mgL⁻¹) completely within 27 and 12 h respectively. Various parameters like pH, temperature, NaCl and initial dye concentrations were optimized. The maximum concentration of Orange 16 (1000 mgL⁻¹) was decolorized by strains ETL-A and ETL-B within 78 and 84 h respectively. These strains could decolorize Orange 16 over a broad pH range 5.5–9.0; the optimum pH was 7.2. The decolorization of Orange 16 was most efficient at 40°C. Analysis of biodegradation products carried out with UV–vis spectroscopy, HPTLC and FTIR confirmed the decolorization and degradation of Orange 16. Further, both the strains showed the involvement of azoreductase in the decolorization process. These results indicate the effectiveness of strains ETL-A and ETL-B for the treatment of textile effluents containing azo dyes.

Keywords:
Orange 16 Bacillus Pseudomonas HPTLC FTIR

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