Study of the Tissue Reaction of the Region of the Midpalatine Suture of Rats after Disjunction with Spring Anchored in Mini-Implants

Agenor Osório¹, Roberto Rosendo Barros Reis², Suzimara Reis Gea Osorio¹, Adriana Neves Da Costa³, Karina Maria Salvatore Freitas^4, and Marcelo Cavenaghi Pereira Da Silva^{2, 5}

¹Uninga University Center, Maringá, Brazil

²São Leopoldo Mandic, Campinas, Brazil

³Universidade Federal de São Paulo, Brazil

⁴Dentistry, Uninga University Center, Maringá, Brazil

⁵Morfofunctional Sciences, University of São Paulo, Brazil

Cite this paper:
Agenor Osório, Roberto Rosendo Barros Reis, Suzimara Reis Gea Osorio, Adriana Neves Da Costa, Karina Maria Salvatore Freitas and Marcelo Cavenaghi Pereira Da Silva. Study of the Tissue Reaction of the Region of the Midpalatine Suture of Rats after Disjunction with Spring Anchored in Mini-Implants. International Journal of Dental Sciences and Research. 2017; 5(4):98-109. doi: 10.12691/ijdsr-5-4-4

Abstract

Midpalatine suture disjunction is a routine procedure in the orthodontic clinic; however, the cellular events involved in the procedure have not yet been completely explained. The aim of this study was to verify the tissue events, particularly those related to osteogenic differentiation, which occur in the region of the palatal suture after disjunction, by means of histologic and immunohistochemical analyses of the following noncollagenous proteins: BSP, OCC, OPN and ONC. In this study, 10 male Wistar rats aged two months and weighing approximately 250 g were used. The animals were divided into two groups of five animals: Group I – control (mini-implant placement) and group II – experimental (placement of mini-implant and disjunction spring made of 0.60mm steel wire, with an initial load of 2N (200 grams)). After 21 days, the animals were sacrificed and histological and immunohistochemical analyses were performed for the noncollagenous proteins. The use of the spring anchored to mini-implants promoted the disjunction of the median palatal suture, with a V-shaped opening, in which the base faced the superior region (at the nasal cavity) and the vertex was in the inferior region of the oral cavity (at the palatal bone). It could be concluded that after disjunction, there was presence of osteoblasts in an osteoid matrix in the region of the median palatal suture, and the immunohistochemical expression of the noncollagenous proteins increased in all the analyzed parameters, expression being most significant for ONC.

Keywords:
rapid maxillary expansion anchorage mini-implants noncollagenous proteins

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References:

[1]	Haas AJ. Rapid expansion of the maxillary dental arch and nasal cavity by opening the midpalatine suture. Angle Orthod 1961; 31(2): 73-90.
[2]	Langlade M. Terapêutica Ortodôntica. São Paulo: Santos; 3. ed. 1995. p. 322-3.
[3]	Silva Filho OG, Pinheiro Junior JM, Cavassan AO. Comportamento dos incisivos centrais superiores após a expansão rápida da maxila na dentadura mista: um estudo piloto longitudinal radiográfico. Rev Dental Press Ortodon Ortoped Facial 1997; 2(1): 68-85.
[4]	Hahn L, Marchioro EM, Rizzatto SD et al. Avaliação do volume da cavidade nasal antes e após a expansão rápida da maxila por meio da rinometria acústica. Rev Sogaor 1999; 3(2): 85-96.
[5]	Garib DG, Henriques JFC, Janson G et al. Avaliação da expansão rápida da maxila por meio da tomografia computadorizada: relato de um caso. Rev Dental Press Ortodon Ortop Facial 2005; 10(4): 34-46.
[6]	Biederman W, Chem B. Rapid correction of Class III malocclusion by midpalatine expansion. Am J Orthod 1973 Jan; 63(1): 47-55.
[7]	Umemori M. Skeletal anchorage system for open bite correction. Am J Orthod 1999; 115(2): 166-74.
[8]	Roberts WE, Helm FR, Marshal KJ. Rigid endosseous implants for orthodontic and orthopedic anchorage. Angle Orthod 1989; 59: 247-56.
[9]	Kanomi R. Mini-implant for orthodontic anchorage. J Clin Orthod 1997 Nov; 31(11): 763-767.
[10]	Kyung HM, Hong SG, Park YC. Distalization of maxillary molars with a midpalatine miniscrew. J Clin Orthod 2003; 1(37): 22-26.
[11]	Parr JA, Garetto LP, Wohlford ME et al. Sutural expansion using rigidly integrated edosseous implants: na experimental studdy in rabbits. Angle Orthodont 1997; 67(4): 283-90.
[12]	Harzer W, Schneider M, Gedrange T. Rapid maxillary expansion with palatal anchorage of the hyrax expansion screw-pilot study with case preresentation. J Orofac Orthop 2004; 65(5): 419-24.
[13]	Nefussi JR, Brami G, Modrowski D et al. Sequential expression of bone matrix proteins during rat calvaria osteoblast differentiation an bone nodule formation in vitro. J Histochem Cytochem 1997; 45 (4): 493-503.
[14]	Hiraki KRN. Estudo da expressão imunoistoquímica das proteínas não-colagênicas da matriz extracelular óssea e dos fatores de transcrição CBFA1 e SOX9 em osteossarcomas e condrossarcomas de maxila e mandíbula, SP [tese]. São Paulo: Faculdade de Odontologia da Universidade de São Paulo; 2007.
[15]	Martinez EF, Araújo VC. In vitro immunoexpression of extracellular matrix proteins in dental pulpal and gingival human fibroblasts. Int Endod J 2004; 37(11): 749-55.
[16]	Haas AJ. The treatment of da maxillary deficiency by opening the midpalatine suture. Angle Orthod 1965; 35: 200-17.
[17]	Capelozza Filho L, Taniguchi SM, Silva Filho OG. Expansão rápida e tração extrabucal reversa da maxila na dentadura mista: comentários através de caso clínico. Ortodontia 1990 set-dez; 23(3): 66-78.
[18]	Proffit WR, Fields Jr HW. Ortodontia Contemporânea. São Paulo: Guanabara-Koogan; 2002. seção IV – Biomecânica e Mecânica, 279-340.
[19]	Consolaro A, Martins-Ortiz MF, Ennes JP et al. O Periósteo e a Ortopedia dos maxilares. Rev Dental Press Ortodon Ortop Facial 2001 jul-ago; 6(4); 77-89.
[20]	Araújo AM, Buschang PH. Conceitos atuais sobre o crescimento e desenvolvimento transversal dos maxilares e oportunidade de expansão mandibular. Rev Dental Press Ortodon Ortop Facial 2004; 9(3): 122-36.
[21]	Del Santo JRM. Por que considerar crescimento craniofacial? Minhas opções clínicas: Uma perspectiva baseada em evidências. In: Nova visão em Ortodontia / Ortopedia funcional dos maxilares. São Paulo: Santos; 2006.
[22]	Viazis AD. Entrevista. Rev Dental Press Ortodon Ortop Facial 1996 jul-ago; 3(4): 1-2.
[23]	Proffit WR. Ortodontia contemporânia. São Paulo: Pancast; 1991. p. 171-222.
[24]	Haas AJ. Entrevista. Rev Dental Press Ortodon Ortop Facial 2001 jan-fev; 1(6): 1-10.
[25]	Silva Filho OG, Hernandes R, Okada T. Efeitos induzidos pela expansão rápida da maxila sobre pré-molares de ancoragem. Ortodontia 1994; 3(27): 18-36.
[26]	Zimring JF, Isaacson RJ. Forces produced by rapid maxillary expansion III. Forces present during retention. Angle Orthod 1965 July; 35(3): 178-186.
[27]	Silva Filho OG, Capelozza Filho L. Expansão rápida da maxila: preceitos clínicos . Ortodontia 1988; 21(1): 49-69.
[28]	Martins DR, Henriques JFC, Velásquez NZ et al. Aparelho tipo HYRAX colado: uma outra alternativa para o tratamento da mordida cruzada posterior. Rev Dent Press Ortodont Ortop Facial 1998 set-out; 3(5): 41-44.
[29]	Capelozza Filho L, Silva Filho OG. Expansão rápida da maxila: considerações e aplicações clínicas. In: Interlandi S. Ortodontia: bases para iniciação. São Paulo: Artes Médicas; 1999. cap. 17, 285-328.
[30]	Enlow DH, Hans MG. Noções básicas sobre crescimento facial. Trad. Terezinha Oppido. São Paulo: Santos; 1998. cap. 2: Conceitos Básicos de Crescimento. p. 18-36, cap. 5: O Complexo Nasomaxilar. p. 79-96.
[31]	Vardimon AD, Brosh T, Spiegler A et al. Rapid palatal expansion: part 1.mineralization pattern of the midpalatine suture in cats. Am J Orthod 1998; 4(113): 371-8.
[32]	Thilander B, Rygh P, Reitan K. Diagnóstico e Planejamento do tratamento Ortodôntico. In: Graber TM, Vanarsdall RL. Ortodontia princípios e técnicas atuais. 3. ed. Rio de janeiro: Guanabara Koogan; 2002. cap. 2: Reações teciduais em ortodontia. p. 101-168.
[33]	Chambers TJ, Hall TJ. Cellular and molecular mechanisms in the regulation and function of osteoclasts. Vitam Horm 1992; (46): 41-86.
[34]	Meghji S. Bone remodeling. Br Dent J 1992 Mar; 6(172): 235-42.
[35]	Riggs BL, Melton LJ. The prevention and treatment of osteoporosis. N Engl J Méd 1992 Aug; 9(327): 620-7.
[36]	Hill PA. Remodelação Óssea. Rev Dental Press Ortodon Ortop Facial 1999 mar-abr; 2(4): 56-62
[37]	Seibel M. Biochemical Markers of Bone Turnover Part I: Biochemistry and Variability. Clin Biochem Rev 2005; 26(4): 97-122.
[38]	Butler WT, Brunn JC, Qin C et al. Dentin Extracellular Matrix (ECM) Proteins: Comparison to Bone ECM and Contribution to Dynamics of Dentinogenesis. Connect Tissue Res 2003; 44(1): 171-78.
[39]	Meyer U, Meyer T, Vosshans J et al. Decreased expression of osteocalcin and osteonectin in relation to high strains and decreased mineralization in mandibular distraction osteogenesis. J Cranio Maxillof Surg 1999; 27(4): 222-7.
[40]	Christenson RH. Biochemical markers of bone metabolism: an overview. Clin Biochem 1997; 30(8): 573-593.
[41]	Vieira JGH. Considerações sobre os marcadores bioquímicos do metabolismo ósseo e sua utilidade prática. Arq Bras Endocrinol Metab 1999; 43(6): 415-422.
[42]	Warren SM, Mehrara BJ, Steinbrech DS et al. Rat mandibular distraction osteogenesis. Part III. Gradual distraction versus acute lenthning. Plast Reconstr Surg 2001; 107: 441-53.
[43]	Uemura T, Nemoto A, Liu YK et al. Osteopontin involvement in bone remodeling and its effects on invivo osteogenic potential of bone marrow-derived osteoblasts/porous hydroxyapatite constructs. Mater Sci Eng 2001; 17: 33-36.
[44]	Morinobu M, Ishijima M, Rittling S. Osteopontin expression in osteoblasts and osteocytes during bone formation under mechanical stress in the calvarial suture in vivo. J Bone Miner Res 2003; 18: 1706-1715.
[45]	Perrien DS, Brown EC, Aronson J et al. Immunohistochemical study of osteopontin expressing during distraction osteogenesis in the rat. J Histochem Cytochem 2002; 50(4): 567-574.
[46]	Siqueira FM. Distribuição dos componentes não colágenos da matriz extracelular em tumores odontogênicos, SP [tese]. São Paulo: Faculdade de Odontologia da Universidade de São Paulo; 2006.