Evaluation of the Antibacterial Effect of MTAD and Sodium Hypochlorite against Endodontic Pathogens

Ghada Y. Abdul Rahman^1, , Karam Ahmed Thiab² and Rasha M. Al-Shamaa²

¹Department of Dental Basic Science, College Of Dentistry, Mosul University, Iraq

²Department of Conservative Dentistry, College of Dentistry, Mosul University, Iraq

Cite this paper:
Ghada Y. Abdul Rahman, Karam Ahmed Thiab and Rasha M. Al-Shamaa. Evaluation of the Antibacterial Effect of MTAD and Sodium Hypochlorite against Endodontic Pathogens. International Journal of Dental Sciences and Research. 2014; 2(2):47-49. doi: 10.12691/ijdsr-2-2-5

Abstract

The purpose of this investigation was to compare the efficacy of Biopure MTAD versus 5.25% NaOCl as irrigant solutions for root canals infected with E. faecalis and Strept. Mutans. Materials and Methods: Forty extracted human single-rooted lower premolar teeth are decoronated at the cemento-enamel junction (CEJ). The roots will instrumented by k-file till size 40 then these teeth will contaminated by bacteria in brain heart infusion for 48 hrs. The samples will divide into 3 groups (MTAD, NaOCl and normal saline). Dentin chips will removed from the canal with sterile low speed hand piece round bur the collected dentin chips will transfer by BHI and culture on the media and the growing colonies will count and record as colonies forming unit (CFU). Results: The antibacterial activity of Biopure MTAD was significantly differ from that of 5.25% NaOCl and distilled water.Conclusion: Biopure MTAD has the strongest antibacterial effect against E.faecalis and Strept. mutans than 5.25% NaOCl and distilled water.

Keywords:
disinfection of root canal Enterococcus faecalis MTAD

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