Estimation of Interleukin 8 in Aphthous Ulceration

Arjwan M. Shuker¹, Wael T. Al-Wattar² and Mahmoud Y.M. Taha^1,

¹Department of Dental Basic Sciences, College of Dentistry, Mosul University, IRAQ

²Department of Oral Surgery, College of Dentistry, Mosul University, IRAQ

Cite this paper:
Arjwan M. Shuker, Wael T. Al-Wattar and Mahmoud Y.M. Taha. Estimation of Interleukin 8 in Aphthous Ulceration. International Journal of Dental Sciences and Research. 2021; 9(2):37-41. doi: 10.12691/ijdsr-9-2-4

Abstract

Recurrent aphthous ulceration (RAU) is an acute inflammatory disease affecting oral mucosa. It has been reported that cytokines such as IL2, TNF α, IL6 were produced in patients with RAU. IL8 is a neutrophil chemotactic factor and its level increases in response to virus and bacterial products and TNF α or IL1β production. The aim of the study was to estimate the concentration of IL-8 in RAU patients compared to control group. The study was carried out on a total number of 70 individuals of both sexes and different age groups (50 patients and 20 control ) attending Department of Oral Surgery, Unit of Oral Medicine, College of Dentistry /Mosul University for the period from 1/10/2017 to 1/4/2018. Clinical examination was performed and blood samples collected, serum was separated and used in ELISA test to estimate IL-8 concentration. The location of RAU was common in the lower lips followed by buccal mucosa. The concentration of IL-8 in RAU patients was highly significant compared to control group. Within different age groups, IL-8 was higher in age group 13-30 years old. Also high concentration of IL-8 was noticed in females more than males. Minor RAU showed higher concentration of IL-8.

Keywords:
RAU IL-8

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References:

[1]	Scully C, and Porter S. Oral Mucosal Disease: Recurrent Aphthous. Brit J Oral Maxill Surg. 46(3): 198-206. 2008.
[2]	Axell T and Henricsson V. The Occurrence of Recurrent Aphthous Ulcers in an adult Swedish Population. Act Odontol Scand. 43: 121-125. 1985.
[3]	Saraceno R1, Perugia C, Ventura A, LORè B, Chimenti S, Docimo R.. Aphthous, celiac disease and other dental disorders in childhood. G Ital Dermatol Venereol. 151(3): 239-243. 2016.
[4]	Belenguer-Guallar I, Jime´nez-Soriano Y, and Claramunt-Lozano A. Treatment of recurrent aphthous stomatitis: A literature review. J Clin Exp Dent. 6(2): 168-174. 2014.
[5]	Slebioda Z, Szponar E, Kowalska A. Etiopathogenesis of recurrent aphthous stomatitis and the role of immunologic aspects: literature review. Arch Immunol Ther Exp (Warsz). 62(3): 205-215. 2014.
[6]	Buno IJ, Huff JC, Weston WL. Elevated Levels of Interferon Gamma, Tumor Necrosis Factor Alpha, Interleukins 2, 4, and 5, but not Interleukin 10, are present in Recurrent Aphthous Stomatitis. Arch. Dermatol. 134(7): 827-831. 1998.
[7]	Sun A, Chang YF, Chia JS, Chiang CP. Serum interleukin-8 level is a more sensitive marker than serum interleukin-6 level in monitoring the disease activity of recurrent aphthous ulcerations. J Oral Pathol Med. 33: 133-139. 2004.
[8]	Katsantonis J, Adler Y, Orfanos CE, Zouboulis CC. Adamantiades-Behçet's disease: Serum IL-8 is a more reliable marker for disease activity than C-reactive protein and erythrocyte sedimentation rate. Dermatol. 201: 37-39. 2000.
[9]	Brozovic S, Vucicevic, Boras V, and Bukovic D. Serum IgA, IgG, IgM and salivary IgA in recurrent aphthous ulceration. Coll Anthropol. 25(2): 633-637. 2001.
[10]	Martinez OK, Mendes LL, Alves BJ. Secretory A immunoglobulin, total proteins and salivary flow in Recurrent Aphthous Ulceration. Rev Bras Otorrinol. 73(3): 323-328. 2007.
[11]	Okada H, and Murakami S. Cytokine expression in periodontal health and disease. Crit Rev Oral Biol Med. 9: 248-266. 1998.
[12]	Sun A, Wang JT, Chia JS, Chiang CP. Levamisole can modulate the serum tumor necrosis factor-alpha level in patients with recurrent aphthous ulcerations. J Oral Pathol Med. 35: 111-116. 2006.
[13]	Al-Dalaan A, al-Sedairy S, al-Balaa S, al-Janadi M, Elramahi K, Bahabri S et al. Enhanced interleukin 8 secretion in circulation of patients with Behçet's disease. J Rheumatol. 22: 904-907. 1995.
[14]	Sun A, Chu CT, Liu BY, Wang JT, Leu JS and Chiang CP. Expression of Interleukin-2 Receptor by Activated Peripheral Blood Lymphocytes Upregulated by the Plasma Level of Interleukin-2 in Patients with Recurrent Aphthous Ulcers. Proc. Natl. Sci. Counc. Repub. China B. 24(3): 116-122. 2000.
[15]	Bakhtiari B. Vaziri PB, Hajilooi M, Mortazavi H. Evaluation of serum levels of interleukin-6 and interleukin-8 in patients with recurrent aphthous ulcerations. Rev of Glob Med Healthcare Res. 1(1): 410. 2010.
[16]	Gür-Toy G, Lenk N, Yalcin B, Aksaray S, Alli N. Serum interleukin-8 as a serologic marker of activity in Behçet's disease. Int J Dermatol. 44(8): 657-660. 2005.
[17]	Sun A, Chang YF, Chia JS, Chiang CP. Serum interleukin-8 level is a more sensitive marker than serum interleukin-6 level in monitoring the disease activity of recurrent aphthous ulcerations. J Oral Pathol Med 33: 133-139. 2004.
[18]	Trotter A, Mück K, Grill H,Schirmer UW, Hannekum A, Lang D. Gender-related plasma levels of progesterone, interleukin-8 and interleukin-10 during and after cardiopulmonary bypass in infants and children. Critical Care. 5(6): 343-348. 2002.
[19]	DaPozzo E, Giacomelli C, Cavallini C, and Claudia Martini C. Cytokine secretion responsiveness of lymphomonocytes following cortisol cell exposure: Sex differences. PLoS One. 13(7): e0200924. 2018.
[20]	Ship JA, Chavez EM, Doerr PA, Henson BS, Sarmadi M. Recurrent aphthous stomatitis. Quintessence Int. 31: 95-112. 2000.
[21]	M K PD, D N S V R, Koppal S, Byatnal AR, Rukmangada T, Byatnal AA. Efficacy of Rebamipide and Levamisole in the Treatment of Patients with Recurrent Aphthous Ulcer - A Comparative Study. J Clin Diagn Res. 8(11): ZC119-ZC122. 2014.
[22]	McRobbie H, Hajek P, Gillison F. The relationship between smoking cessation and mouth ulcers. Nicotine Tob Res. 6: 655-665. 2004.
[23]	Abdullah MJ. Prevalence of recurrent aphthous ulceration experience in patients attending Pyramid dental speciality in Sulaimani City. J ClinExp Dent. 5(2): e89-e94. 2013.