Flexural Strength, Antimicrobial Activity and Color Stability of Ginger (zingiber officinale) Modified Heat Cured Denture Base Material

Neven S. Aref^{1, 2,} , Mohamed M. Said³, Rasha H. El-Mahdy⁴ and Noha A. El-Wassefy¹

¹Dental Biomaterials Department, Faculty of Dentistry, Mansoura University, Mansoura, Egypt

²Basic Oral and Medical Sciences Department, Faculty of Dentistry, Qassim University, Qassim, KSA

³Removable Prosthodontics Department, Faculty of Dentistry, Mansoura University, Mansoura, Egypt

⁴Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Cite this paper:
Neven S. Aref, Mohamed M. Said, Rasha H. El-Mahdy and Noha A. El-Wassefy. Flexural Strength, Antimicrobial Activity and Color Stability of Ginger (zingiber officinale) Modified Heat Cured Denture Base Material. International Journal of Dental Sciences and Research. 2019; 7(1):10-17. doi: 10.12691/ijdsr-7-1-3

Abstract

Objective: This study evaluated ginger modified heat cured poly methyl methacrylate (PMMA) denture base material regarding; surface roughness, flexural strength, antimicrobial activity, and color stability. Materials and methods: Ginger was used in powder and oil forms to modify the polymer and monomer. Seven experimental groups were formulated; Group (I) control; Group (II) 3 wt% ginger-powder modified polymer; Group (III) 5 wt% ginger-powder modified polymer; Group (IV) 3 v/v% ginger-oil modified monomer; Group (V) 5 v/v% ginger-oil modified monomer; Group (VI) 3 wt% ginger-powder modified polymer mixed with 5 v/v% ginger-oil modified monomer; Group (VII) 5 wt% ginger-powder modified polymer mixed with 3 v/v% ginger-oil modified monomer. Specimens were tested for surface topography, surface roughness, flexural strength, antimicrobial activity, and color stability. The data were statistically analyzed by one-way ANOVA and post-hoc LSD analysis with a significance factor of α = 0.05. Results: The PMMA polymer modified with 3 v/v% ginger-oil (Group IV) exhibited a significant reduction in surface roughness, a significant increase in flexural strength with clinically acceptable color change. No antimicrobial activity was detected for any of the evaluated groups. Conclusions: 3 v/v% ginger-oil was able to improve the mechanical properties of heat cured PMMA denture base resin with a tolerable color change.

Keywords:
flexural strength ginger PMMA reinforced surface roughness

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References:

[1]	Braden M. Some aspects of the chemistry and physics of dental resins. Adv Dent Res 1988; 2: 93-97.
[2]	Dixon DL, Ekstrand KG, Breeding LC. The transverse strengths of three denture base resins. J Prosthet Dent 1991; 66: 510-513.
[3]	Fujii K. Fatigue properties of acrylic denture base resins. Dent Mater J 1989; 8: 243-259.
[4]	Beyli MS, von Fraunhofer JA. An analysis of causes of fracture of acrylic resin dentures. J Prosthet Dent 1981; 46: 238-241.
[5]	Yazdanie N, Mahood M. Carbon fiber acrylic resin composite: An investigation of transverse strength. J Prosthet Dent 1985; 54: 543-547.
[6]	Radford DR, Sweet SP, Challacombe SJ, Walter JD. Adherence of Candida albicans to denture base materials with different surface finishes. J Dent 1998; 26: 577-583.
[7]	Yamauchi M, Yamamoto K, Wakabayashi M, Kawano J. In vitro adherence of microorganisms to denture base resin with different surface texture. Dent Mater J 1990; 9: 19-24.
[8]	Barbeau J, Séguin J, Goulet JP,de Koninck L, Avon SL, Lalonde B, Rompré P, Deslauriers N.Reassessing the presence of Candida albicans in denture-related stomatitis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2003; 95: 51-59.
[9]	Quirynen M, Bollen CML, Papaioannou W, Van Eldere J, van SteenbergheD.The influence of titanium abutment surface roughness on plaque accumulation and gingivitis: short-term observations. Int J Oral Maxillofac Implants 1996; 11: 169-178.
[10]	Bollen CML, Lambrechts P, Quirynen M. Comparison of surface roughness of oral hard materials to the threshold surface roughness for bacterial plaque retention: a review of the literature. Dent Mater 1997; 13: 258-269.
[11]	da Silva FC, Kimpara ET, Mancini MN, Balducci I, Jorge AO, Koga-Ito CY. Effectiveness of six different disinfectants on removing five microbial species and effects on the topographic characteristics of acrylic resin. J Prosthodont 2008; 17: 627-633.
[12]	Goiato MC, Zuccolotti BC, Moreno A, dos Santos DM, Pesqueira AA, Dekon SF. Colour change of soft denture liners after storage in coffee and coke. Gerodontology 2011; 28: 140-145.
[13]	Imirzalioglu P, Karacaer O, Yilmaz B, OzmenMsc I. Color stability of denture acrylic resins and a soft lining material against tea, coffee, and nicotine. J Prosthodont 2010; 19: 118-124.
[14]	Jin C, Nikawa H, Makihira S, Hamada T, Furukawa M, Murata H.Changes in surface roughness and colour stability of soft denture lining materials caused by denture cleansers. J Oral Rehabil. 2003; 30: 125-130.
[15]	Sivasothy Y, Chong WK, Hamid A, Eldeen IM, Sulaiman SF, Awang K. Essential oils of Zingiber officinale var. rubrum Theilade and their antibacterial activities. Food Chemistry 2011; 124: 514-517.
[16]	Soković M, Glamočlija J, Marin PD, Brkić D, van Griensven LJ. Antibacterial effects of the essential oils of commonly consumed medicinal herbs using an in vitro model. Molecules 2010; 15: 7532-7546.
[17]	Stević T, Berić T, Šavikin K, Sokovic M, Godevac D, Dimkic I, Stankovic S. Antifungal activity of selected essential oils against fungi isolated from medicinal plant. Industrial Crops and Products 2014; 55: 116-122.
[18]	Silva AC, Lopes PM, Azevedo MM, Costa DC, Alviano CS, Alviano DS. Biological activities of a-pinene and β-pinene enantiomers. Molecules 2012; 17: 6305-6316.
[19]	Nesterenko A, Alric I, Silvestre F, Durrieu V. Vegetable proteins in microencapsulation: A review of recent interventions and their effectiveness. Industrial Crops and Products 2013; 42: 469-479.
[20]	Pero AC, Ignárcio J, Giro G, Mendoza-Marin DO; Paleari AG; Compagnoni MA. Surface properties and color stability of an acrylic resin combined with an antimicrobial polymer. Revista de Odontologia da UNESP 2013; 42: 237-242.
[21]	Alla RK, Sajjan S, Alluri VR, Ginjupalli K, Upadhya N.Influence of fiber reinforcement on the properties of denture base resins. J Biomater Nano Biotech 2013; 4: 91-97.
[22]	Racoti A, Rusen E, Diacon A, Dinescu A, Buleandra M, Calinescu I.Ginger Essential Oil Encapsulation in PMMA Microcapsules. Part I. MATERIALE PLASTICE 2016; 53: 357-360.
[23]	Mane S, Ponrathnam S, Chavan N. Effect of Chemical Cross-linking on Properties of Polymer Microbeads: A Review Canadian Chemical Transactions 2015; 3: 473-485.
[24]	Racoti A, Rusen E, Dinescu A, Ion VA, Calinescu I, Mocanu A, Diacon A. Ginger Essential Oil Encapsulation in PMMA Microcapsules. PART II. U.P.B. Sci. Bull., Series B 2016; 78: 87-96.
[25]	Hargreaves AS. Nylon as a denture-base material. Dent Pract Dent Rec 1971; 22: 122-128.
[26]	Oliveira LV, Mesquita MF, Henriques GEP, Consani RL, Fragoso WS. Effect of polishing technique and brushing on surface roughness of acrylic resins. J Prosthodont 2008; 17: 308-311.
[27]	Feng QL. A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J Biomed Mater Res 2000; 54: 662-668.
[28]	Jung WK. Antibacterial activity and mechanism of action of the silver ion in Staphylococcus aureus and Escherichia coli. Appl Environ Microbiol 2008; 74: 2171-2178.
[29]	Chaffin WL, López-Ribot JL, Casanova M, Gozalbo D, Martínez JP. Cell wall and secreted proteins of Candida albicans: identification, function, and expression. Microbiol Mol Biol Rev 1998; 62: 130-180.
[30]	Yassen D, Ibrahim AE. Antibacterial Activity of Crude Extracts of Ginger (Zingiber officinale Roscoe) on Escherichia coli and Staphylococcus aureus: A Study in vitro. Indo American Journal of Pharmaceutical Research 2016; 6: 5830-5835.
[31]	Sah P, Al-Tamimi B, Al-Nassri N, Al-Mamari R. Effect of temperature on antibiotic properties of garlic (Allium sativum L.) and ginger (Zingiber officinale Rosc.). African Journal of Biotechnology 2012; 11: 16192-16195.
[32]	Silva PM, Acosta EJ, Jacobina M, Pinto Lde R, Porto VC. Effect of repeated immersion solution cycles on the color stability of denture tooth acrylic resins. J Appl Oral Sci 2011; 19: 623-627.
[33]	Rosentritt M, Esch J, Behr M, Leibrock A, Handel G. In vivo color stability of resin composite veneers and acrylic resin teeth in removable partial dentures. Quintessence Int 1998; 29: 517-522.
[34]	Kiat-amnuay S, Johnston D, Powers J, Jacob RF. Color Stability of Dry Earth Pigmented Maxillofacial Silicone A‐2186 Subjected to Microwave Energy Exposure.J Prosthodont 2005; 14: 91-96.