SCREENING OF ANTIVIRAL ACTIVITY STUDIES FROM ROOT AND LEAF LATEX EXTRACTS OF ALOE SINANA REYNOLDS: AN ENDEMIC PLANT SPECIES OF ETHIOPIA

Akalu Terfa^1, , Hailemichael Tesso², Anuradha. G³, J. Sreekanth kumar⁴, Sang Un Choi⁵, Sang- Ho Lee⁵ and Young-Sik Jung⁶

¹Adama Science and Technology University, Adama, Post Box No: 1888, Oromiya, Ethiopia.

²Adama Science and Technology University, Adama, Post Box No: 1889, Oromiya, Ethiopia.

³Adama Science and Technology University, Adama, Post Box No: 1890, Oromiya, Ethiopia.

⁴Department of Environmental Sciences, Acharya Nagarjuna University, A.P, India.

⁵Bio and Drug Discovery Division, Korea Research Institute of Chemical Technology, Sinseongno, Yuseong, Daejeon 305-600, South Korea.

⁶Cancer and Infectious Diseases Therapeutics Research Group, Korea Research Institute of Chemical Technology, Sinseongno, Yuseong, Daejeon 305-600, South Korea.

Cite this paper:
Akalu Terfa, Hailemichael Tesso, Anuradha. G, J. Sreekanth kumar, Sang Un Choi, Sang- Ho Lee and Young-Sik Jung. SCREENING OF ANTIVIRAL ACTIVITY STUDIES FROM ROOT AND LEAF LATEX EXTRACTS OF ALOE SINANA REYNOLDS: AN ENDEMIC PLANT SPECIES OF ETHIOPIA. International Journal of Clinical and Diagnostic Research. 2020; 8(1):13-24. doi: 10.12691/ijcdr-8-1-2

Abstract

Crude methanol extract and its organic solvent fractions of n-hexane (HxF), chloroform (CHF), ethylacetate (EAF) and n-butanol (BuF) of Aloe sinana root and leaf latex were tested in- vitro for antiviral activity against influenza A and B viruses, Picornavirus including Rhinovirus, Coxsackievirus, Enterovirus and Denguevirus using cytopathic effect (CPE) reduction assay for influenza virus and Picornavirus, an immune fluorescence assay (IFA) for DENV-2. The antiviral assays demonstrated that MeOH extract, HxF and EAF of A. sinana root and CHF of A. sinana leaf latex significantly inhibited influenza A and B viruses-induced CPE and increased the cell viability of Vero cells. Among these EAF of A. sinana root and CHF of A. sinana leaf latex exhibited a high activity against influenza A and B virus resulting in more potent than that of reference antiviral reagent Ribavirin. BuF of A. sinana root exhibited strong activity against Enterovirus 68 and 71 (H). A. sinana root significantly inhibited DENV-2 production at a higher concentrations as well as DENV-2 induced CPE at lower concentration and increased the cell viability of Vero cells. A. sinana leaf latex significantly inhibited DENV-2 induced CPE at both concentrations and strongly inhibited DENV-2 production at higher concentrations. This data suggests that these extracts contain components which may be effectively utilized as wide-spectrum antiviral agents which support the folkloric assertion of the plant. A. sinana an endemic plant of Ethiopia should permit the identification of bioactive compounds possessing specific activities that may be useful in the development of new and effective antiviral agents.

Keywords:
Endemic plant Antiviral activity Influenza virus Picornavirus Dengue virus

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