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Biomedical Science and Engineering. 2014, 2(1), 5-12
DOI: 10.12691/bse-2-1-2
Open AccessReview Article

Different in Vitro Activation Methods for Latent Transforming Growth Factors (TGF)–β: Considerable Exogenous Factors to Promote Higher Mesenchymal-Origin Cell Proliferation in a Bioprocessing Platform

Partha S. Saha1, and Michael Doran2

1Department of Biomedical Sciences, Faculty of Medicine, University of Leuven (KU Leuven), Leuven, Belgium

2School of Biomedical Sciences, Faculty of Health, Queensland University of Technology (QUT), Brisbane, Australia

Pub. Date: February 12, 2014
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Cite this paper:
Partha S. Saha and Michael Doran. Different in Vitro Activation Methods for Latent Transforming Growth Factors (TGF)–β: Considerable Exogenous Factors to Promote Higher Mesenchymal-Origin Cell Proliferation in a Bioprocessing Platform. Biomedical Science and Engineering. 2014; 2(1):5-12. doi: 10.12691/bse-2-1-2

Abstract

Regenerative medicine includes two efficient techniques, namely tissue-engineering and cell-based therapy in order to repair tissue damage efficiently. Most importantly, huge numbers of autologous cells are required to deal these practices. Nevertheless, primary cells, from autologous tissue, grow very slowly while culturing in vitro; moreover, they lose their natural characteristics over prolonged culturing period. Transforming growth factors-beta (TGF-β) is a ubiquitous protein found biologically in its latent form, which prevents it from eliciting a response until conversion to its active form. In active form, TGF-β acts as a proliferative agent in many cell lines of mesenchymal origin in vitro. This article reviews on some of the important activation methods-physiochemical, enzyme-mediated, non-specific protein interaction mediated, and drug-induced- of TGF-β, which may be established as exogenous factors to be used in culturing medium to obtain extensive proliferation of primary cells.

Keywords:
latent TGF-β activation extensive cell culture exogenous factors hMSC proliferation bioreactor

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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