American Journal of Zoological Research
ISSN (Print): 2373-678X ISSN (Online): 2373-6771 Website: https://www.sciepub.com/journal/ajzr Editor-in-chief: Apply for this position
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American Journal of Zoological Research. 2013, 1(1), 1-4
DOI: 10.12691/ajzr-1-1-1
Open AccessArticle

Qualitative Analysis of Accessory Glands Secretory Proteins in Different Species of Drosophila

Suhasini L. Kudupali1 and Shivanna N.2,

1Department of Zoology, Karnatak University, Dharwad, India

2Department of Applied Genetics, Karnatak University, Dharwad, India

Pub. Date: March 09, 2013

Cite this paper:
Suhasini L. Kudupali and Shivanna N.. Qualitative Analysis of Accessory Glands Secretory Proteins in Different Species of Drosophila. American Journal of Zoological Research. 2013; 1(1):1-4. doi: 10.12691/ajzr-1-1-1

Abstract

Qualitative analysis of male accessory gland secretory proteins in 11 species of Drosophila was analyzed using SDS-PAGE technique and their molecular weight was calculated with the help of marker proteins, the molecular weight ranges from 12-134 kD. The accessory gland secretions of D. virilis and D. hydei reveal more number of protein fractions. D. rajasekari showed less number of ACP fractions. Out of 14 protein fractions of D. virilis 5 were glycosylated whereas in D. hydei none of the fractions were glycosylated. In D. rajasekari 2 fractions were glycosylated. In D. bipectinata, D. malerkotlina and D. ananassae even though the accessory glands produced 7, 8 and 11 protein fractions only one protein fraction is glycosylated. These results showed that the number of ACP fractions and their glycosylation is species specific.

Keywords:
drosophilaaccessory gland qualitative analysis CBB stain glycosylated fractions PAS stain

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References:

[1]  Chen, P. S, “The functional morphology and biochemistry of insect male accessory glands and their secretions”. Annual review of Entomology, 29. 233-55. 1984.
 
[2]  Chapman, K. B. and Wolfner, M. F, “Determination of male-specific gene expression in Drosophila accessory glands”. Developmental Biology, 126. 195-202. 1988.
 
[3]  Chapman, T and Davies, S. J, “Functions and analysis of the seminal fluid proteins of male Drosophila melanogaster fruit flies”. Peptides, 25(9), 1477-1490. 2004.
 
[4]  Fehrnstrom, H. and Moberg, U, “SDS and conventional polyacrylamide gel electrophoresis with LKB 2117 multiphor. LKB instruments”. Inc. Stockholm. 1977.
 
[5]  Shivanna, N. and Ramesh, S. R, “Quantitative and qualitative analysis of accessory gland secretory proteins in a few species of immigrans group”. Indian Journal of Experimental Biology, 33. 668-672. 1995.
 
[6]  Hiremani, R. B. and Shivanna, N, “Accessory gland secretory proteins in relation to fitness parameters of Drosophila ananassae and Drosophila varians. Entomological research, 40. 251-258. 2010.
 
[7]  Ravi Ram, K. and Ramesh, S. R, “Male accessory gland secretion in D. nasuta subgroup: qualitative and quantitative correlations”. Drosophila Information Service, 85. 1-3. 2002.
 
[8]  Ravi Ram, K. and Ramesh, S. R, “Male accessory gland secretory proteins in nasuta subgroups of Drosophila: Nature and SDS-PAGE patterns”. Indian Journal of Experimental Biology, 37. 767-773. 1999.
 
[9]  Shivanna, N., Siddalingamurthy, G. S. and Ramesh, S. R, “Larval pupation site preference and its relation to glue protein in few species of Drosophila”. Genome, 39. 105-111. 1996.
 
[10]  Segrest, J. P. and Jackson, R. L, “Molecular weight determination of glycoproteins by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. In Methods in Enzymology” (ed. Ginsburg, V.), Academic press New York, 28. 54-63. 1972.
 
[11]  Bairati, A, “Structure and ultrastructure of the male reproductive system in Drosophila melanogaster Meig. 2. The genital duct and accessory glands”. Monitore Zoologico. Italiano. (N.S.). 2. 105-182. 1968.
 
[12]  Federer, H. and Chen, P. S, “Ultrastructure and nature of secretory proteins in the male accessory gland of D. funebris. Journal of Insect Physiology, 28. 743-51. 1982.
 
[13]  Frank, W. A., Laura, K. S., Brooke, A. L., Dustin Rubinstein, C. and Mariana, F. W, “Insect seminal fluid proteins identification and function”. Entomology, 56. 21-40. 2011.
 
[14]  Markow, T. A. and O’Grady, P. M, “Drosophila biology in the genomic age”. Genetics. 177. 1269-1276. 2007.
 
[15]  Gillott, C, “Male accessory gland secretions: Modulators of female reproductive physiology and behavior”. Annual review of Entomology, 48. 163-184. 2003.
 
[16]  Ravi Ram, K and Ramesh, S. R, “Male accessory gland secretory proteins in a few members of the D. nasuta subgroup”. Biochemical genetics, 39. 99-115. 2001.