Structural Changes in Intervertebral Discs at Chronic Staphylococcus Aureus Osteomyelitis of the Tibia

Zhurakovsky I. P.¹, Arkhypov S. A.¹, Pustovetova M. G.¹ and Kunts T. A.^1,

¹Central Research Laboratory, Novosibirsk State Medical University, Novosibirsk, Russia

Cite this paper:
Zhurakovsky I. P., Arkhypov S. A., Pustovetova M. G. and Kunts T. A.. Structural Changes in Intervertebral Discs at Chronic Staphylococcus Aureus Osteomyelitis of the Tibia. American Journal of Medicine Studies. 2013; 1(3):28-31. doi: 10.12691/ajms-1-3-4

Abstract

Inflammatory process is not limited only local effects but could affect other organs and tissues. In the present study, we investigated the influence of tibial osteomyelitis caused by Staphylococcus aureus on extracellular matrix components of Wistar rat’s intervertebral discs. Histochemical assay was carried out on sulfated glycosaminoglycans, neutral glycoproteins and collagen fibers of nucleus pulposus. Immunochemical method was applied to I and II type collagen, fibronectin and fibulin-2. The role of persisting staphylococcal infection in the initiation and development of degenerative changes of the fibrous cartilage of intervertebral discs was demonstrated. Progressive disorders in sulfated glycosaminoglycans metabolism accompanied by changes of a fibrous component and collagen type predominance replacement suggest fibrous tranformation in intervertebral discs. Increase of neutral glycoproteins due to separate fractions, in particular, fibulin-2 could be considered as compensatory reaction on progressing overpatchings of fibrocartilage extracellular matrix components. Reorganizations mentioned are supposed to promote further dystrophic-degenerative changes in intervertebral discs.

Keywords:
intervertebral disks nucleus pulposus bacterial infection staphylococcus aureus collagen glycosaminoglycans neutral glycoproteins fibronectin fibulin-2

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

Figures

References:

[1]	Adams, M.A., Roughley, P.J., “What is intervertebral disc degeneration, and what causes it?”, Spine, 31. 2151-2161. 2006.
[2]	Anderson, D.G., Izzo, M.W., Hall, D.J., Vaccaro, A.R., Hilibrand. A., Arnold, W., Tuan, R.S., Albert, T.J “Comparative gene expression profiling of normal and degenerative discs: analysis of a rabbit annular laceration model”, Spine, 27 (12). 1291-1296. 2002.
[3]	Arkhipov, S.A., Shkurupy, V.A., Solomatina, M.V., Akhramenko, E.S., Iljine, D.A., “Study of macrophages in BCG granulomas in different compartments of the mononuclear phagocyte system”, Bull. Exp. Biol. Med., 154 (4). 467-470. Feb. 2013.
[4]	Bibeau, F., Boissière-Michot, F., Sabourin, J.C., “Assessment of epidermal growth factor receptor (EGFR) expression in primary colorectal carcinomas and their related metastases on tissue sections and tissue microarray”, Virchows Arch, 449 (3). 281-287. 2006.
[5]	Clouet, J., Grimandi, G., Pot-Vaucel, M., Masson, M., Fellah, H.B., Guigand, L., Cherel, Y., Bord, E., Rannou, F., Weiss, P., Guicheux, J., Vinatier, C. “Identification of phenotypic discriminating markers for intervertebral disc cells and articular chondrocytes”, Rheumatology (Oxford). 48 (11). 1447-1450. 2009.
[6]	Fukuta, S. “Abundance of calpain and aggrecan-cleavage products of calpain in degenerated human intervertebral discs”, Osteoarthritis Cartilage, 19 (10). 1254-1262. Oct. 2011.
[7]	Green, B.N. “Methicillin-resistant Staphylococcus aureus: an overview for manual therapists”, J. Chiropr. Med., 1 (11). 64-76. 2012.
[8]	Gruber, H.E. “Variations in aggrecan localization and gene expression patterns characterize increasing stages of human intervertebral disk degeneration”, Exp Mol Pathol., 2 (91). 534-539. 2011.
[9]	Kadler K.E., Hill A., Canty-Laird E.G. “Collagen fibrillogenesis: fibronectin, integrins, and minor collagens as organizers and nucleators”, Curr. Opin. Cell Biol. 20 (5). 495-501. 2008.
[10]	Kalson, N.S., Richardson S., Hoyland J.A. “Strategies for regeneration of the intervertebral disc”, Regen. Med., 3. 717-729. 2008.
[11]	Klevens, R.M. “Invasive methicillin-resistant Staphylococcus aureus infections in the United States”, JAMA, 15 (298). 1763-1771. 2007.
[12]	Komandenko, N.I. “Modeling of osteochondrosis of the spinal column”, Biull. Eksp. Biol. Med., 6 (125). 630-632. 1998.
[13]	Komandenko, N.I., Ryzhov, A.I., Zhurakovsky, I.P. “Degenerative disk desease”. Sibmedizdat NSMU, Novosibirsk, 2006. 246 p.
[14]	Li S., Van Den Diepstraten C., D’Souza S.J., Chan B.M.C., Pickering J.G. “Vascular smooth muscle cells orchestrate the assembly of type I collagen via alpha2beta1 integrin, RhoA, and fibronectin polymerization”, Am. J. Pathol. 163. 1045-1056. 2003.
[15]	Mathieu O., Cruz-Orive, L.M., Hoppeler, H., Weibel, E.R. “Measuring error and sampling variation in stereology: Comparison of the efficiency of various methods of planar image analysis”, J. Microsc., 121 (1). 75-88. 1981.
[16]	McManus, J.F.A. “The histological and histochemical uses of periodic acid”, Stain Technol., 23. 99-108. 1948.
[17]	Miyamoto, K. “Intradiscal injections of osteogenic protein-1 restore the viscoelastic properties of degenerated intervertebral discs”, Spine J., 6. 692-703. 2006.
[18]	Zhao, C.Q. “The cell biology of intervertebral disc aging and degeneration”, Ageing Res Rev., 3 (6). 247-261. 2007.
[19]	Zhurakovsky, I.P., Pustovetova, M. G., Kunts, T. A., Bitkhaeva, M. V. “Tissue microregion of the liver at restorative period after tibia traumatic injury”, Kursk’s scientific bulletin “Man and health”, 4. 34-38. 2011.
[20]	Zhurakovsky, I.P., Aidagulova, S.V., Ishenko, I.Yu., Pustovetova, M.G. “Intervertebral discs morphology at staphylococcal infection modelling”, NSU Bulletin, Biol., Clin. Med., 11 (3). 102-108. 2013.