Induction of Abscess by Non-abscess Producer Staphylococci Using Staphylococcal Fatty Acid Modifying Enzyme (FAME)

Raga I. O.^1, , Gameel A. A.² and El-Sanousi S. M.³

¹Department of Microbiology, Faculty of Veterinary Science, University of Nyala, P. O. Box 155, Nyala Sudan

²Department of Pathology, Faculty of Veterinary Medicine, University of Khartoum, P. O. Box 32, Khartoum Sudan

³Department of Microbiology, Faculty of Veterinary Medicine, University of Khartoum, P. O. Box 32, Khartoum Sudan

Cite this paper:
Raga I. O., Gameel A. A. and El-Sanousi S. M.. Induction of Abscess by Non-abscess Producer Staphylococci Using Staphylococcal Fatty Acid Modifying Enzyme (FAME). American Journal of Microbiological Research. 2018; 6(1):33-37. doi: 10.12691/ajmr-6-1-5

Abstract

Stahpylococcus epidermidis (S. epidermidis), S. warneri and S. aureus subsp. anaerobious were isolated from sheep abscess. Crude FAME was extracted from supernatant fluid of a 24hrs culture of S. aureus subsp. anaerobious grown in Trypticase Soy Broth at 37°C with continuous agitation. Fourteen heads of sheep were used. Three of them were inoculated with S. epidermidis + FAME and other three were inoculated with S. warneri + FAME. Eight animals were divided into four groups, two animals in each, used as control and inoculated with S. epidermidis, S. warneri, S. aureus subsp. anaerobious and FAME. All animals were inoculated subcutaneously at the neck region. After fourteen to eighteen days, sites inoculated with S. epidermidis + FAME, S. warneri + FAME and S. aureus subsp. anaerobious showed abscesses grossly then confirmed bateriologically and histopathologically, while those inoculated with S. epidermidis alone, S. warneri alone and FAME showed no abscess.

Keywords:
Stahpylococcus abscess fatty acid modifying enzyme

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References:

[1]	Barrow, G. I. and Feltham R. K. A. (2003). Cown and Steel,s Manual for the Identification of Medical Bacteria. 3rd edition. Cambridge University.
[2]	Chamberlain, N. R. and Imanoel, B. (1996) Genetic regulation of fatty acid modifying enzyme from Staphylococcus aureus. J. of Med. Microbiol., 44 (2):125-129. Press.
[3]	Chamberlain, N. R. (1999). Identification and partial characterization of an extracellular activator of fatty acid modifying enzyme (FAME) expression in Staphylococcus epidermidis. J. of Med. Microbiol., 48: 245-252.
[4]	Dye, E.S.and Kapral, F.A. (1981). Survival of Staphylococcus aureus in intraperitoneal abscess. J. Med. Microbiol., 14: 185-194.
[5]	Kapral, F. A.; Smith, S. and Lal, D. (1992). The esterification of fatty acids by Staphylococcus aureus fatty acid modifying enzyme (FAME) and its inhibition by glycerides. J. of Med. Microbiol., 37: 235-237.
[6]	Long, J. P.; Hart, J.; Albers, W. and Kapral, F. A. (1992). The production of fatty acid modifying enzyme (FAME) and lipase by various Staphylococcal species. J. of Med. Microbiol., 37: 232-234.
[7]	Mortensen, J. E.; Shryock, T. R. and Kapral, F. A. (1992). Modification of bactericidal fatty acids by an enzyme of Staphylococcus aureus. J. of Med. Microbiol., 36:293-298.
[8]	Saeed, K. B. and El-Sanousi, S. M. (1995). Scheme for identification of Staph. Species protocol, Department of Microbiology, Fac. of Vet. Science, Univ. of Khrtoum. (Personal communication).
[9]	Thea Lu, Joo Youn Park, Kelleen Parnell, Larry K Fox, and Mark A McGuire (2012). Characterization of fatty acid modifying enzyme activity in staphylococcal mastitis isolates and other bacteria. J. of BMC Res Notes, 5: 323.