Exopolysaccharide (EPS) Production by Exiguobacterium aurantiacum isolated from Marchica Lagoon Ecosystem in Morocco

Y. D. Dah Dossounon^1, , K. Lee¹, K. Belghmi¹, F. Benzha¹ and M. Blaghen¹

¹Laboratory of Microbiology, Pharmacology, Biotechnology and Environment, University Hassan II, Faculty of Sciences Ain-Chock, route El Jadida, B.P. 5366, Casablanca, Morocco

Cite this paper:
Y. D. Dah Dossounon, K. Lee, K. Belghmi, F. Benzha and M. Blaghen. Exopolysaccharide (EPS) Production by Exiguobacterium aurantiacum isolated from Marchica Lagoon Ecosystem in Morocco. American Journal of Microbiological Research. 2016; 4(5):147-152. doi: 10.12691/ajmr-4-5-4

Abstract

Exiguobacterium aurantiacum, a member of the Bacilli class, has the ability to synthesize and secrete exopolysaccharides. The strain, isolated from Marchica lagoon in Morocco, produced exopolysaccharide (EPS), mainly during its exponential growth phase but also to a lesser extent during the stationary phase. The optimum pH and temperature for growth and exopolysaccharide (EPS) production were 8 and 37°C respectively, the dry weight of the exopolysaccharides products and biomass was found to be 259. 05 ± 1.48 mg/100ml and 150.25 ± 0. 35 mg/100ml respectively. The partially purified exopolysaccharide (EPS) samples were chemically analyzed. The results showed that the temperature and pH have no effect on the protein amount produced by E. aurantiacum while the carbohydrate amount varied. The functional groups in the partially purified exopolysaccharides were determined by the FT-IR. Because of its ability to produce large quantities of exopolysaccharides; this bacterium may prove to be an excellent model species for the development of biotechnology products.

Keywords:
exopolysaccharide exiguobacterium aurantiacum marchica lagoon FT-IR

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