Biodegradation of Dimethylformamide Using Bacillus Subtilis

R. Vidhya^1, and A.J. Thatheyus²

¹PG Department of Immunology and Microbiology, The American College, Madurai, India

²PG and Research Department of Zoology, The American College, Madurai, India

Cite this paper:
R. Vidhya and A.J. Thatheyus. Biodegradation of Dimethylformamide Using Bacillus Subtilis . American Journal of Microbiological Research. 2013; 1(1):10-15. doi: 10.12691/ajmr-1-1-3

Abstract

The present study investigated the potential of the chosen bacterium, a natural isolate Bacillus subtilis isolated from textile industry effluent (textile effluent adapted bacterium) and the isolate was found to be more efficient in degrading DMF based on the assessment of physico-chemical parameters like pH, turbidity, carbon dioxide and ammonia released during the degradation process. DMF degradation has led to the accumulation of ammonia and dimethylamine contributing to the increase of pH of the medium from 7.0 to 9.2. Increase in turbidity and biomass was also observed during the treatment. The maximum release of carbon dioxide and ammonia was found during the degradation of 100µl of DMF. HPLC analysis for 200µl of DMF degradation by the isolate showed peaks with different retention times. Thus the results indicated that the isolate was able to degrade DMF found in the textile industrial effluents.

Keywords:
dimethylformamide textile effluent bacillus subtilis degradation HPLC

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