Using Water with Oil Immersion Lens to Detect Malaria Parasite in Blood Film and Making a Comparison between Oil and Water Method

Hiba Siddig Ibrahim^1, and Shamsoun Khamis Kafi¹

¹Department of microbiology, Faculty of Medical Laboratory Science, The National Ribat University

Cite this paper:
Hiba Siddig Ibrahim and Shamsoun Khamis Kafi. Using Water with Oil Immersion Lens to Detect Malaria Parasite in Blood Film and Making a Comparison between Oil and Water Method. American Journal of Microbiological Research. 2014; 2(4):122-124. doi: 10.12691/ajmr-2-4-4

Abstract

Background: Oil immersion lens it is one of the microscope lenses that used in medical laboratory filed. It is used to magnify the smallest things and for detection of some important infectious diseases such as bacteria, parasite, anemia’s and bone marrow films, but uses lens alone without oil makes the image imperfect and unclear, so to make a better image scientists were used oil with these lenses (immersion oils lens) to avoid bending of light . Objectives: The main aim of this study is to be used distill water with oil immersion lens instead of synthetic oil to detect malaria parasite to low cost of using synthetic oil especially in faraway places due to difficulties of paying synthetic oil and also to be arrived to rural area, and so on to be used with other tests, beside avoiding using impurity oil due to the comment of D/ Fahad Awad (National Coordinator for Malaria Program), against using sesame oil especially in some laboratories at Al Gedaraf State, see Aray Alaam newspaper 5th December, 2013. Methodology: In this study a total of 200 subjects were included. A 3 drops from capillary blood samples were collected in clean dry dust free slides after disinfectant the 3ed or 4th finger for adult and the big toe hand or foot for child with 70% alcohol to make a thick blood films, or Aliquots of 2.5 ml of venous blood were collected by venous puncture after disinfectant the site of collection and the collected blood was drawn into EDITA containers to make thick blood films as above. The films were lets to dry by air, staining with Giemsa stain for 10 minute and then examined for the first times with DW with objective lens and for the second times with synthetic oil. Results: There were no differences between using oil or distill water (DW) for detection of malaria parasite by both techniques (100%), unless the high quality of oil image (100%), when comparing with distill water (DW) image (90%), due to variation in numerical aperture (NA) between both techniques. Conclusion: Based on the results of this study we can used distill water (DW) but with caution to detect malaria parasite by 90% when compared with oil 100% in case of poverty areas to low cost of using synthetic oil, and so on the image quality can arrives (99.5%) if do it in proper way.

Keywords:
distill water (DW) objective lens bending numerical aperture (NA)

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References:

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