Testing the Quality of Biofertilizer Illetrisoy

Tutik Prihast^1,

¹Indonesian Legume and Tuber Crops Research Institute, Malang, East Java, Indonesia

Cite this paper:
Tutik Prihast. Testing the Quality of Biofertilizer Illetrisoy. American Journal of Microbiological Research. 2014; 2(2):63-67. doi: 10.12691/ajmr-2-2-4

Abstract

This is a descriptive analytical study, aims to assess the quality of bio-fertilizers Illetrisoy up to viability resilience bacterial during the storage. Illetrisoy A and B are the two types of biological fertilizers that contains of beneficial bacterial to improving growth and productivity of soybean. The research conducted covers the identification of the microbial are contained in the biological fertilizer Illetrisoy, the characterization of carrier matter and the viability of bacteria on the carrier matter during storage in different of packaging (plastic bag, allumunium foil, and plastic bottle) and storage temperature (refrigerator, room temperature, and incubator 40°C). The research shows that Illetrisoy A and Illetrisoy B contain 4 species of bacteria from the genus Phaenibacillus and Bacillus. The carrier of Illetrisoy is a mixture of RawaPening peat soil and charcoal powders provides a good quality in Illetrisoy A (pH 6.75, C 22.13 g kg^-1, N 1.32 g kg^-1, P₂O₅ 389 mg kg^-1, K 2.68 cmol kg^-1and microbial population 36.5 x 10⁸cfu g^-1 of matter) and Illetrisoy B (pH 6.92, C 21.74 g kg^-1, N 1.25 g kg^-1, P₂O₅ 412 mg kg^-1, K 2.47 cmol kg^-1and microbial population 34.9 x 10⁸cfug^-1 of matter). The product of biological fertilizer Illetrisoywith water content 50-60 % could be stored in the plastic packaging bags and be placed in refrigerator temperature 4^oC. On the conditions of storage, the product of biological fertilizers Illetrisoy can be survived for 3 months, with the viability of bacteria preserved on the total population of 10⁸cfug^-1 of matter.

Keywords:
Illetrisoy quality bio-fertilizer

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References:

[1]	Harsono, A., Subandi, dan Suryantini, 2010. Formulasi pupuk hayati dan organik untuk meningkatkan produktivitas aneka kacang 20%, ubi 40 % menghemat pupuk kimia 50%. Laporan Penelitian 2010. Balitkabi.53 hal.
[2]	Husen, E., R.D.M. Simanungkalit, and Irawan. 2007. Characterization and quality assessment of Indonesian commercial biofertilizers. Indonesian Journal of Agricultural Science 8:31-38.
[3]	Makarim, K., F. Islam, M. A. Akkas Ali, F. Haque. 2001. On-farmtrail with Rhizobium inoculants on lentil. Bangladesh J. Agric Res 26, 93-94.
[4]	Husen, E. 2009. Telaah efektivita spupuk hayati komersial dalam meningkatkan pertumbuhan tanaman. Prosiding Seminar dan Lokakarya Nasional Inovasi Sumberdaya Lahan, Bogor 24-25 November 2009. Balai Besar Penelitian dan Pengembangan Sumberdaya Lahan Pertanian, Badan Penelitian dan Pengembangan Pertanian. Pp. 418-423.
[5]	Anas, I., D. A. Santoso dan Y. Fakuara. 1992. Bioteknologi Pertanian 2. Pusat Antar Universitas Bioteknologi, Institut Pertanian Bogor, Bogor. P. 187-327.
[6]	Jin H. J., R. Tu, F. Xu, and S. F. Chen. 2011. Identification of Nitrogen Fixing Paenibacillus from Different Plant Rhizospheres and a Novel nifH Gene Detected in the P. stellifer1, Microbiology, 80 (1): 117-124.
[7]	Chung YR, C. H. Kim, I. Hwang, and J. Chun. 2000. Paenibacilluskoreensis sp. nov., a new species that produces an iturin-like antifungal compound. Int. J. Syst. Evol. Microbiol. 50: 1495-1500.
[8]	Hoult, B., and A. F. Tuxford. 1991. Toxin production by Bacillus pumilus. J ClinPathol (44): 455-458. Downloaded from jcp.bmj.com on November 8, 2012.
[9]	Aslim, B., N. Saglam, and Y. Beyatli. 2002. Determination of some properties of Bacillus isolated from soil. Turk. J. Biol. (26): 41-48
[10]	Awais, M., A. A. Shah, A. Hameed, and F. Hasan. 2007. Isolation, Identification and Optimization of Bacitracin Produced by Bacillus sp. Pak. J. Bot., 39 (4): 1303-1312.
[11]	Akhtar, M. S., U. Shakeel, Z. A. Siddiqui. 2010. Biocontrol of Fusariumwilt by Bacillus pumilus, Pseudomonas alcaligenes, and Rhizobium sp. on lentil. Department of Botany, Aligarh Muslim University, Aligarh – INDIA. Turk J Biol (34): 1-7.
[12]	De-Bashan, L. E., J. P. Hernandez, Y. Bashan, and R. M. Maier. 2010. Bacillus pumilus ES4: Candidate plant growth-promoting bacterium to enhance establishment of plant in mine tailings. Environmental and Experimental Botani (69): 343-352.
[13]	Afzal, A. and B. Asghari, 2008. Rhizobium and phosphate solubilizing bacteria improve the yield and phosphorus uptake in wheat (Triticumaestivum L). Int. J. Agric. Biol., 10: 85-8.
[14]	Ahmed, Z.I., M. Ansar, M. Tariq and M.S. Anjum, 2008. Effect of different Rhizobium inoculation methods on performance of lentil in pothowar region. Int. J. Agric. Biol., 10: 85-8.
[15]	Kennedy, A.C. 1998. The rhizosphere and spermosphere. Pp 389-407 In SILVIA et al. (Eds.) Principles and Application of Soil Microbiology. Prentice Hall. New Jersey.
[16]	Jasinski, S.M. 2000. Peat. US Geologycal Survey: US Departement of the interior.
[17]	Syekhfani. 2010. Hubungan hara tanah, air dan tanaman. Dasar-dasar pengelolaan tanah subur berkelanjutan. Putra Media Nusantara. 205 hlm.
[18]	Simanungkalit, R.D.M., R.J. Roughley, and A. Indrasumunar. 1999. The effect of carrier material and moisture potential on the quality of legume inoculants. Penelitian Pertanian Tanaman Pangan 18(1): 64-70.
[19]	Prihastutidan A. Harsono. 2012. Kemunduran kualitas pupukhayati Rhizobium. J. Sains dan Matematika 1 (1): 1-5.