Enzymatic Activity of Actinomycetes Isolated from the Soil of the Public Landfill of Lifoula (Republic of Congo)

Mboukou Kimbatsa Irène Marie Cecile^{1, 2}, Ignoumba Evariste Mesmin^{1, 3}, Gatsé Elgie Viennechie^{1, 3,} , Nkounkou Bendo Yvestha Hanselme^{1, 3}, Morabandza Cyr Jonas^{1, 3} and Nguimbi Etienne^{1, 3}

¹Laboratory of Microbiology, Infectiology and Immunology, ENS-UMNG, B.P 69 Brazzaville

²Laboratory of Remote Sensing and Forest Ecology, ENS-UMNG, B.P 69 Brazzaville

³Laboratory of Cellular and Molecular Biology, FST-UMNG, B.P 69 Brazzaville

Cite this paper:
Mboukou Kimbatsa Irène Marie Cecile, Ignoumba Evariste Mesmin, Gatsé Elgie Viennechie, Nkounkou Bendo Yvestha Hanselme, Morabandza Cyr Jonas and Nguimbi Etienne. Enzymatic Activity of Actinomycetes Isolated from the Soil of the Public Landfill of Lifoula (Republic of Congo). American Journal of Microbiological Research. 2024; 12(6):120-126. doi: 10.12691/ajmr-12-6-1

Abstract

Actinomycetes are Gram-positive filamentous bacteria that have colonized even the most hostile ecosystems. To date, they remain the main source of secondary metabolites and are currently at the origin of the development of several fields such as medical biology, pharmacology and ecology. This work aimed at the characterization of actinomycetes isolated from the soil of the public landfill of Lifoula (Brazzaville); with a view to the possible discovery of rare strains or strains with high enzymatic production potential for industrial use. Soil samples were taken from 2 different points of the landfill, between 5 and 10 cm deep. Inoculation on specific media was followed by enumeration, isolation, phenotypic identification of isolates and enzyme production using an agar well technique. The results showed loads of 70 and 73.30 x 104CFU/g on starch-casein medium compared to 53.30 and 56.60 x 104CFU/g on starch-casein enriched with yeast extract for samples 1 and 2, respectively. 20 isolates were recovered including 9 purified isolates: 6 isolates from sample 1 and 3 from sample 2. The skim milk clotting test was positive for 8 isolates and negative for isolate A2. Enzyme production diameters were amylase, 20 mm for isolates A1 and A7 compared to 14 mm for A4, A9 and A10; proteases, 38 to 50 mm for isolates A1, A2, A7 and A8 compared to 19 mm for A3, A4 and A6 and no production in A9 and A10; Cellulase, 20 mm for A3, A4, A6, A7, A8 and A10 compared to 15 mm for A1, A2 and A9; Lipases 30 mm for A6, A9 and A10 compared to 20 mm for A1, A2 and A8 and 15 mm for A3 and A4. These results suggest that the soil of the Lifoula landfill is rich in enzyme-producing actinomycetes, which can be used in a number of fields, including the food, textile and pharmaceutical industries.

Keywords:
actinomycetes enzymes starch-casein yeast extract Lifoula

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]	Duchaufour P., Faivre P., Poulenard J., Gury M., 2020, «Introduction à la science du sol»,Sol, végétation, environnement; Dunod; Paris; 450p.
[2]	Smaoui, S. 2010. Purification et Caractérisation de Biomolécules à partir de microorganismes nouvellement isolés et identifiés. Thèse de doctorat: Génie de Procédés et Environnement.Tunisie: Université de Toulouse, p251Ameur H., 2014: «effet d’osmoprotecteurs naturels sur la restauration de croissance de Streptomyces et de plantes d’intérêts agricole sur le sol salé ou aride», Thèse de doctorat, Université Ferhat Abbas Sétif 1, 200p + annexes.
[3]	Amir A., 2015, «Activité antagoniste de deux souches de Streptomyces à l’égard d’une nouvelle collction de germes pathogènes isolées au niveau du CHU (Bejaia)», Mémoire de Master, Université Abder Ahmane Mira-Bejaia, Algérie, 104p.
[4]	Lechevalier M P, Lechevalier H., 1970, “Chemical composition as a criterion in the classification of aerobic actinomycetes”, International Journal of Systemic Bacteriology, 20: 435–443p.
[5]	Delarras C., 2007, “Pratique en microbiologie de laboratoire”, Edition Lavoisier, Paris, 190-209p.
[6]	Nguimbi E., Ahombo G., Moyen R., Ampa R.; Vouidibio A., Ontsira E. N., Kobawila S. C., Louembe D. (2014). Optimization of Growth, Fibrinolytic Enzyme Production and PCR Amplification of Encoding Fibrinolytic Enzyme Gene in Bacillus amyloliquefaciens Isolated from Ntoba mbodi at Brazzaville. International Journal of Science and Research (IJSR), Volume 3 Issue 11, 2319-7064.
[7]	TatsinkouFossi B., Tavea F., Ndjouenkeu R, 2005, “Production and Partial Characterization of Thermostable Alpha Amylase from Ascomycete Yeast Strain Isolated from Starchy Soil”, Afr Journal Biotechnol, 14–18p.
[8]	Boudemagh A., 2007, «Isolement à partir des sols Sahariens, de bactéries actinomycétales productrices de molécules antifongiques, identification moléculaire de souches actives», Thèse de Doctorat, Université Mentouri Constantine, Algérie, 132p.
[9]	Haritha R., Siva Kumar K., Jagan Mohan Y.S.Y.V., 2010, “Amylolytic and proteolytic actinobacteria isolated from marine sediments of Bay of Bengal” International J Microbiol Res, 1(2): 37–44p.
[10]	Saini P., Gangwar M., Kalia A., Singh N and Narang D., 2016, “Isolation of endophytic actinomycetes from Syzygium cumini and their antimicrobial activity against human pathogens”. Journal of Applied and Natural Science,8, (1): 416 – 422p.
[11]	Boucheffa K., 2011, «Criblage de souches d’actinomycètes productrices d’antifongiques non-polyénique: identification des souches productrices et Essai de caractérisation des antifongiques produits», Mémoire Magistral, Université Abderrahmane Mira Bejaia, Algérie, 90p.
[12]	Saci K. et Safane A., 2015, «Isolement des souches d’actinomycètes d’un sol agricole contaminé par l’herbicide Apros et étude de leur capacité à le dégrader», Mémoire de Master, Université Frères Mentouri Constantine, Algérie, 55p.
[13]	Belferkh A., Megoura M., 2016, «Isolement des actinomycètes à partir d’un sol Saharien et d’une Sebkha de la region d’El-Ouest et mise en évidence de leur capacité à dégrader quelques pesticides», Mémoire de Master, Université des Frères Mentouri Constantine, Algérie, 52p.
[14]	Boudjella H., Bouti K., Zitouni A., Mathieu F., Lebrihi A. and Sabaou N., 2007, “Taxonomy and chemical characterization of antibiotics of Streptosporangium Sg 10 isolated from a Saharan soil”, Microbiol, Res, 161: 288-298p.
[15]	Balasubramaniam, V., Ganesh, S., Karunanithi, V., Perumal, P, 2011, “Impoved Culturing, Screening and Fermentation of soil Actinomycetes for Actinomycrobial Agents”, Int. J.Pharm and Ind. Res Vl (01) Issue (02): 153-159p.
[16]	Belabed, B, 2014, «Recherche des activités anti-pathogènes chez les espèces du genre Streptomyces isolées de différents biotopes», Mémoire magistère: Biologie moléculaire et génétique des microorganismes. Oran, Université d’Oran, 98p.
[17]	Segouat A. et Boukhakfa R. K. E., 2018, «Isolement d’actinomycètes à partir de deux échantillons de sol et la mise en évidence de quelques activités enzymatiques», Mémoire de Master, Université des Frères Mentouri Constantine, Algérie, 47p.
[18]	Habibeche L., 2013, “Isolement et selection des souches d’actinomycètes poductrices d’antibiotique”, Mémoire de master, Université Abderrahmane Mira de Bejiai, Algérie, 50p.
[19]	Lazouni F. et Ferhaoui W. S., 2022, «Isolement et selection des souches d’actinomycètes d’origine marine productrices d’antibiotiques», Mémoire de master, Université Yahia Fares d Medea, 70p.
[20]	Stamford T.L.M., Stamford N.P., Coelho L.C.B., and Araujo J.M., 2001, “Production and characterization of a thermostable -amylase from Nocardiopsis sp. endophyte of yam bean, Bioresource Technology, 19(4): 225–233p.
[21]	Moussi A.et Soumia K, 2021, « Caractérisation phénotyque et étude préliminaire des activités enzymatique et inhibitrices des actinomycètes du sol », Mémoire de master, Université Mohamed Khider de Biskra,40p.
[22]	Mihaela C., Teodor Gh., Negoita., Gabriela E., Bahrim., Peter Stougaard., 2011, “Partial characterization of cold active amylases and proteases of Streptomyces sp. From Antarctica”, Brazilian journal of microbiology, 42: 868-877p.
[23]	Palaniyandi S.A., Yang S.H., and Suh J.-W., 2013, “Extracellular proteases from Streptomyces phaeopurpureus ExPro13 inhibit spore adhesion, germination and appressorium formation in Colleto trichumcoccodes”. Applied Microbiology, 1364-5072p.
[24]	Boughachiche F., Boulahrouf A., Rachedi K., Duran R., Lauga B., Karama S., B Lynda., Boulezaz S., Boukrouma M., Boutaleb H., 2016, “Optimization of alkaline protease production by Streptomyces sp. strain isolated from saltpan environment”, african journal of biotechnology,15(26):1401-1412p.
[25]	Janaki T., 2017, “Enzymes from Actinomycetes”, International Journal of ChemTechResearc, 10(2): 176-182p.
[26]	Minotto E., Pasqualini M.L., Trindade O.M. and Van Der Sand S.T., 2014, “Enzyme characterization of endophyto-actinobacteria isolated from tomato plants”, Journal of Advanced Scientific Research, 5 (2): 16-23p.
[27]	Benzekhroufa A., 2018, «Contribution à l’étude cinétique des extraits enzymatiques de quelques Streptomyces isolées du sol de la région de Mostaganem», Mémoire de master, Université Abdelhamid Ibn Badis-Mostaganem, 53p.