The Dynamics of Microbes from Surface Water in Doubeli, Adamawa, Northeastern Nigeria

Jennifer A. Tyndall^1, , Jamiu S. Olumoh², Victory Inyang¹, Bwala John Audu³ and Amina Abbas Muhammad¹

¹Department of Natural & Environmental Sciences, American University of Nigeria

²Department of Mathematics & Statistics, American University of Nigeria

³Department of Medical Microbiology and Parasitology, Federal Medical Centre, Yola

Cite this paper:
Jennifer A. Tyndall, Jamiu S. Olumoh, Victory Inyang, Bwala John Audu and Amina Abbas Muhammad. The Dynamics of Microbes from Surface Water in Doubeli, Adamawa, Northeastern Nigeria. American Journal of Microbiological Research. 2024; 12(5):110-119. doi: 10.12691/ajmr-12-5-2

Abstract

Microbial contamination plays a critical role in the spread of waterborne diseases, including the recurrent cholera outbreaks in Doubeli, Adamawa. This study, which evaluated the microbial content of surface water used for various anthropogenic activities in Doubeli at five different sites, was assessed over six months and designated as either residential or agricultural. Data was analyzed using descriptive summaries, the Wilcoxon test, and Kruskal-Wallis with Bonferroni adjusted p-values for pairwise comparisons. Escherichia coli, Citrobacter freundii, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Proteus bacterial species were detected. The parasitic worms Schistosoma haemotobium, Ascaris lumbricoides, Ancylostoma duodenale, and Fasciola spp., as well as the protozoan parasites Giardia lamblia and Entamoeba coli, were all identified in this study, alongside the only fungal species, Candida albicans. There were distinct differences in the mean total coliform bacterial (TCB) counts (176.70 versus 22.54 CFU/100ml) and the fecal coliform bacterial (FCB) counts (190.97 versus 20.93 CFU/100ml) in the rainy and dry seasons, respectively; TCB and FCB titres were both above the World Health Organization’s (WHO’s) permissible limits. Run-offs from these study sites are used for drinking and crop irrigation; therefore, the microbial assessment of these water sources can play a pivotal role in curbing epidemics and reducing childhood mortality in Doubeli, Adamawa. This study moreover highlights the need for public health awareness and effective environmental management in the region.

Keywords:
Microbial contamination surface water total coliform bacterial counts fecal coliform bacterial counts and environmental management

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References:

[1]	Bibi, S., Khan, R. L., Nazir, R., Khan, P., Rehman, H. U., Shakir, S. K., ... & Jan, R. (2016). Heavy metals analysis in drinking water of Lakki Marwat District, KPK, Pakistan. World Applied Sciences Journal, 34(3), 15-19.
[2]	UNICEF (United Nations Children’s Fund). (2022). Universal access to safe drinking water is a fundamental need and human right. https://data.unicef.org/topic/water-and-sanitation/drinking-water/.
[3]	Alavi, N., Zaree, E., Hassani, M., Babaei, A. A., Goudarzi, G., Yari, A. R., & Mohammadi, M. J. (2016). Water quality assessment and zoning analysis of Dez eastern aquifer by Schuler and Wilcox diagrams and GIS. Desalination and Water Treatment, 57(50), 23686-23697.
[4]	Mohammadi, M. J., Salari, J., Takdastan, A., Farhadi, M., Javanmardi, P., Yari, A. R., ... & Rahimi, S. (2017). Removal of turbidity and organic matter from car wash wastewater by electrocoagulation process. Desalination and water treatment, 68, 122-128.
[5]	Derakhshani, E., Naghizadeh, A., Yari, A. R., Mohammadi, M. J., Kamranifar, M., & Farhang, M. (2018). Association of toxicochemical and microbiological quality of bottled mineral water in Birjand city, Iran. Toxin reviews, 37(2), 138-143.
[6]	American Public Health Association. (1926). Standard Methods for the Examination of Water and Wastewater. American Public Health Association.
[7]	Yari, A. R., Mohammadi, M. J., Geravandi, S., Doosti, Z., Matboo, S. A., Jang, S. A., & Nazari, S. (2018). Assessment of microbial quality of household water output from desalination systems by the heterotrophic plate count method. Journal of Water and Health, 16(6), 930-937.
[8]	Burtscher, M. M., Zibuschka, F., Mach, R. L., Lindner, G., & Farnleitner, A. H. (2009). Heterotrophic plate count vs. in situ bacterial 16S rRNA gene amplicon profiles from drinking water reveal completely different communities with distinct spatial and temporal allocations in a distribution net. Water Sa, 35(4), 495-504. https://hdl.handle.net/10520/EJC116631.
[9]	Falcone-Dias, M. F., & Farache Filho, A. (2013). Quantitative variations in heterotrophic plate count and in the presence of indicator microorganisms in bottled mineral water. Food Control, 31(1), 90-96.
[10]	Karbashdehi, V. N., Dobaradaran, S., Soleimani, F., Arfaeinia, H., Mohammadi, M. J., Keshtkar, M., & Mirahmadi, R. (2018). The role of decentralized municipal desalination plants in removal of physical, chemical and microbial parameters from drinking water: a case study in Bushehr, Iran. Journal of Water, Sanitation and Hygiene for Development, 8(2), 325-339.
[11]	WHO (World Health Organization). (2017). Guidelines for drinking-water quality: Fourth edition incorporating the first addendum. https://pubmed.ncbi.nlm.nih.gov/28759192/.
[12]	EPA (Environmental Protection Agency). (2021). Drinking Water Contaminants. https://www.epa.gov/ground-water-and-drinking-water/drinking-water-contaminants.
[13]	WHO (World Health Organization). (2011). Guidelines for drinking-water quality. https:// www.who.int/ water _ sanitation_health/publications/dwq_guidelines/en/.
[14]	Tyndall, J. A., Ndiaye, K., Weli, C., Dejene, E., Ume, N., Inyang, V., ... & Waldman, R. J. (2020). The relationship between armed conflict and reproductive, maternal, newborn and child health and nutrition status and services in northeastern Nigeria: a mixed-methods case study. Conflict and health, 14, 1-15.
[15]	Ngwa, M. C., Wondimagegnehu, A., Okudo, I., Owili, C., Ugochukwu, U., Clement, P., ... & Sack, D. A. (2020). The multi-sectorial emergency response to a cholera outbreak in internally displaced persons’ camps in Borno state, Nigeria, 2017. BMJ Global Health, 5(1), e002000.
[16]	WHO (World Health Organization). (2019). Borno, Adamawa and Yobe states declare end of cholera outbreaks. WHO Africa. https://icanetwork.co.za/borno-adamawa-and-yobe-states-declare-end-of-cholera-outbreaks/.
[17]	Fakhri, Y., Oliveri Conti, G., Ferrante, M., Abbaspour, M., Alinejad, A., Amirhajeloo, L. R., ... & Jeihooni, A. K. (2018). Escherichia coli contamination in groundwater sources of Iran: Systematic review and meta-analysis. Journal of Environmental Health Science and Engineering, 16(1), 1-14.
[18]	Li, G., & Young, K. D. (2013). Indole production by the tryptophanase TnaA in Escherichia coli is determined by the amount of exogenous tryptophan. Microbiology, 159(Pt_2), 402-410.
[19]	Hagan, J. E., Opoku, B. K., Anim-Baidoo, I., Eguavoen, O. I., Obeng-Nkrumah, N., Frempong, M. T., ... & Aryeetey, G. C. (2016). Assessment of microbial contamination of drinking water in the rural Bolgatanga communities of Ghana. Ghana Medical Journal, 50(1), 32-38.
[20]	Bae, S., Wuertz, S., & Yu, X. (2019). Illuminating microbial diversity in surface water systems using 16S rRNA gene sequencing. Journal of Microbiological Methods, 164, 105685.
[21]	Clasen, T., Boisson, S., Routray, P., Torondel, B., Bell, M., Cumming, O., Ensink, J. H. J., Freeman, M. C., Jenkins, M. W., Odagiri, M., Ray, S. K., Sinha, A., Suar, M., Schmidt, W. P., Simpson, H., & Wolf, J. (2015). Effectiveness of a rural sanitation programme on diarrhoea, soil-transmitted helminth infection, and child malnutrition in Odisha, India: a cluster-randomised trial. Lancet Global Health, 3(11), e645-e653.
[22]	Umeh, C. N., Okorie, O. I., & Emesiani, G. A. (2005, November). Towards the provision of safe drinking water: The bacteriological quality and safety of sachet water in Awka, Anambra State. In The book of abstract of the 29th annual conference & general meeting on microbes as agents of sustainable development, organized by Nigerian society for microbiology (NSM), University of Agriculture, Abeokuta (p. 22).
[23]	Egbule, C. L., & Salihu, B. A. (2018). Microbiological assessment of drinking water sources in rural communities in northern Nigeria. International Journal of Community Medicine and Public Health, 5(7), 2756-2761.
[24]	Abakpa, G. O., Umoh, V. J., Ameh, J. B., & Yakubu, S. E. (2013). Microbial quality of irrigation water and irrigated vegetables in Kano State, Nigeria. International Food Research Journal, 20(5).
[25]	Abdulraheem, I. S., Ijaiya, M. A., & Abdulkadir, O. F. (2015). Impact of community-based water quality monitoring on diarrheal morbidity in rural communities in Nigeria. International Journal of Tropical Disease & Health, 9(3), 1-10.
[26]	Aboh, E. A., Giwa, F. J., & Giwa, A. (2015). Microbiological assessment of well waters in Samaru, Zaria, Kaduna, state, Nigeria. Annals of African Medicine, 14(1), 32-38.
[27]	Moini, A. S., Soltani, B., Ardakani, A. T., Moravveji, A., Erami, M., Rezaei, M. H., & Namazi, M. (2015). Multidrug-resistant Escherichia coli and Klebsiella pneumoniae isolated from patients in Kashan, Iran. Jundishapur journal of microbiology, 8(10).
[28]	Khodadadi, M., Mahvi, A. H., Ghaneian, M. T., Ehrampoush, M. H., Dorri, H., & Rafati, L. (2016). The role of desalination in removal of the chemical, physical and biological parameters of drinking water (a case study of Birjand City, Iran). Desalination and Water Treatment, 57(53), 25331-25336.
[29]	Sheth, C. C., Johnson, E., Baker, M. E., Haynes, K., & Mühlschlegel, F. A. (2005). Phenotypic identification of Candida albicans by growth on chocolate agar. Medical mycology, 43(8), 735-738.
[30]	Heidari, S., Basiri, H., Nourmoradi, H., Kamareei, B., & Omidi, Y. (2016). Hexadecyl trimethyl ammonium bromide-modified montmorillonite as a low-cost sorbent for the removal of methyl red from liquid-medium. International journal of engineering, 29(1), 60-67.
[31]	Pontes, L. A., Oliveira, M. C., Katz, N., Dias-Neto, E., & Rabello, A. N. A. (2003). Comparison of a polymerase chain reaction and the Kato-Katz technique for diagnosing infection with Schistosoma mansoni. The American journal of tropical medicine and hygiene, 68(6), 652-656. PMID: 12887022.
[32]	Chukwuneke, C. E., Adams, F. V., Madu, J. O., Agboola, B. O., Inyang, V. F., Olumoh, J. S., ... & Tyndall, J. A. (2022). Evaluation of seasonal variation of heavy metal contamination and health risk assessment in Sabore field Adamawa State, Nigeria. International Journal of Environmental Analytical Chemistry, 102(18), 6640-6654.
[33]	Drzewiecka, D. (2016). Significance and roles of Proteus spp. bacteria in natural environments. Microbial ecology, 72, 741-758.
[34]	Schroth, M. N., Cho, J. J., Green, S. K., Kominos, S. D., & Microbiology Society Publishing. (2018). Epidemiology of Pseudomonas aeruginosa in agricultural areas. Journal of Medical Microbiology, 67(8), 1191-1201.
[35]	Squire, S. A., & Ryan, U. (2017). Cryptosporidium and Giardia in Africa: current and future challenges. Parasites & vectors, 10, 1-32.
[36]	Mahdavi, F., Shams, M., Sadrebazzaz, A., Shamsi, L., Omidian, M., Asghari, A., ... & Salemi, A. M. (2021). Global prevalence and associated risk factors of diarrheagenic Giardia duodenalis in HIV/AIDS patients: A systematic review and meta-analysis. Microbial Pathogenesis, 160, 105202.
[37]	Taghipour, A., Javanmard, E., Haghighi, A., Mirjalali, H., & Zali, M. R. (2019). The occurrence of Cryptosporidium sp., and eggs of soil-transmitted helminths in market vegetables in the north of Iran. Gastroenterology and hepatology from bed to bench, 12(4), 364.
[38]	Prüss-Ustün, A., & World Health Organization. (2008). Safer water, better health: costs, benefits and sustainability of interventions to protect and promote health. World Health Organization.
[39]	Prüss-Ustün, A., Wolf, J., Bartram, J., Clasen, T., Cumming, O., Freeman, M. C., ... & Johnston, R. (2019). Burden of disease from inadequate water, sanitation and hygiene for selected adverse health outcomes: an updated analysis with a focus on low-and middle-income countries. International journal of hygiene and environmental health, 222(5), 765-777.
[40]	Elimian, K. O., Musah, A., Mezue, S., Oyebanji, O., Yennan, S., Jinadu, A., ... & Ihekweazu, C. (2019). Descriptive epidemiology of cholera outbreak in Nigeria, January–November, 2018: implications for the global roadmap strategy. BMC Public Health, 19(1), 1-11.