Isolation and Molecular Detection of Antibiotic Resistant Staphylococcus aureus from Pet Birds of Mymensigh City Corporation Areas, Bangladesh

Sharmin Sultana Royal¹, Sanzila Hossain Sigma¹, Md. Nahid Ashraf¹, Sabrina Sultana Rimi¹, Samia Affroze¹, Salauddin Yousup², Jayedul Hassan¹, Mahbubul Pratik Siddique¹ and Md. Shafiqul Islam^1,

¹Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

²Department of Anatomy and Histology, Faculty of Animal Science and Veterinary Medicine, Habiganj Agricultural University, Habiganj, Bangladesh

Cite this paper:
Sharmin Sultana Royal, Sanzila Hossain Sigma, Md. Nahid Ashraf, Sabrina Sultana Rimi, Samia Affroze, Salauddin Yousup, Jayedul Hassan, Mahbubul Pratik Siddique and Md. Shafiqul Islam. Isolation and Molecular Detection of Antibiotic Resistant Staphylococcus aureus from Pet Birds of Mymensigh City Corporation Areas, Bangladesh. American Journal of Microbiological Research. 2024; 12(1):1-6. doi: 10.12691/ajmr-12-1-1

Abstract

Background: Pet bird (ornamental bird) rearing is gradually increasing in Bangladesh. Pet serves as a best friend and confidant for elderly and young people. For unemployment, pet bird farming acts as an income source. Many people are directly or indirectly involved in this business through pet rearing, breeding, and selling. Antibiotic resistance is a global public health concern. Pet birds are considered one of the reservoirs or carriers of AMR bacteria in humans. As a result, it is necessary to know the risks associated with antibiotic-resistant bacteria for humans who are directly involved in pet bird rearing. This research was performed for the isolation and molecular detection of antibiotic-resistant Staphylococcus aureus (MRSA) from pet birds. Methodology: A total of 169 feces samples, including pigeon (n = 57), budgerigar (n = 56), and cockatiel (n = 56), were collected from different pet shops in Mymensingh city corporation. The bacterial isolates were identified using staining and biochemical assays, followed by molecular identification using PCR. Isolated organisms were then tested for antibiotic sensitivity using disk diffusion methods with ten frequently used antibiotics. Results: Among the 169 samples, 35 (20.71%) were positive for Staphylococcus spp. by conventional and molecular tests. The prevalence of the nuc gene was 33.34% in pigeons, 22.23% in budgerigar, and 7.14% in cockatiel. The prevalence of the mecA gene in S. aureus was 28.57% in pigeons. But no mecA gene was found in budgerigar or cockatiel. S. aureus isolates were resistant to methicillin (100%), vancomycin (71.43%), cotrimoxazole (85.71%), and tetracycline (71.43%). Conclusion: It can be concluded that pet birds harbor enteric bacteria that are resistant to most antibiotics used in our study, and the presence of such antibiotic resistant bacteria in pet birds might pose a potential threat to humans and animals’ health.

Keywords:
pet birds antibiotic resistance Staphylococcus aureus

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