Evaluation of the Antibacterial Potential of the Pomelo (Citrus maxima) Peel Extract Liquid Hand Soap

Ma. Lourdes Betty E. Ochate^1, , Kristel Jane C. Castro¹, Janna Maegan M. Arellano¹, Vince Adolf O. Balagonsa¹, Ian Jay P. Saldo¹ and Mary Jade P. Dandoy¹

¹Integrated Basic Education Department, San Isidro College, Malaybalay City, 8700 Philippines

Cite this paper:
Ma. Lourdes Betty E. Ochate, Kristel Jane C. Castro, Janna Maegan M. Arellano, Vince Adolf O. Balagonsa, Ian Jay P. Saldo and Mary Jade P. Dandoy. Evaluation of the Antibacterial Potential of the Pomelo (Citrus maxima) Peel Extract Liquid Hand Soap. American Journal of Microbiological Research. 2023; 11(2):47-51. doi: 10.12691/ajmr-11-2-3

Abstract

Pomelo (Citrus maxima) has been noticed for its antibacterial activity due to the presence of flavonoids, carotenoids, and phenolic acids, all of which have the potential human health benefits. This study aimed to formulate an antibacterial liquid hand soap made of pomelo (C. maxima) peel extract and evaluate its antibacterial potential. This study utilized a quantitative research design to determine the estimated number of colony-forming units and compare if there is a significant difference before and after using the liquid hand soaps. The antibacterial potential of the hand soap was measured using the standard plate count method. Also, a product evaluation of the formulated liquid hand soap was done using an adapted five-point Likert scale questionnaire. Results revealed a noticeable decrease in the estimated number of colony-forming units after using the hand soaps. Furthermore, mixed measures factorial ANOVA gave a p-value of 0.265, which means no significant difference in the bacterial count between the two liquid hand soaps, as it is greater than the 0.05 level of significance. Therefore, the pomelo (C. maxima) peel extract and commercial liquid hand soap exhibited antibacterial activity in terms of their bacterial count by the standard plate count test. Moreover, the results for the product evaluation showed that between the two liquid hand soaps, the pomelo (C. maxima) peel extract liquid hand soap resulted in a higher average mean for the senior high school and college students and employees' perceptions than the commercial liquid hand soap. However, more research is needed to improve the concentration of the pomelo (C. maxima) peel crude extract to achieve better and more comprehensive results for commercial use.

Keywords:
antibacterial activity bacterial count liquid hand soap pomelo (C. maxima) peel extract product evaluation

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References:

[1]	Doron, S., & Gorbach, S. L. (2017). Bacterial Infections: Overview. Elsevier EBooks, 273-282.
[2]	Al-Saif S. S. A., Abdel-Raouf N., El-Wazanani H., Aref I., 2014 Antibacterial substances from marine algae isolated from Jeddah coast of Red sea, Saudi Arabia. Saudi Journal of Biological Sciences 21(1): 57-64.
[3]	Wijetunge W. M. A. N. K., Perera B. G. K., 2016 Preparation of medicinal soap products using the leaf extracts of Punica granatum (pomegranate). International Journal of Pharmacy and Biological Sciences 6(2): 7-16.
[4]	Singh, A., & Navneet. (2017). Citrus maxima (Burm.) Merr. A Traditional Medicine: Its Antimicrobial Potential And Pharmacological Update For Commercial Exploitation in Herbal Drugs – A Review. International Journal of ChemTech Research, pp 642-651 (0974-4290).
[5]	Ordoyo, T., & Sepe, M. (2019). Antibacterial Potential of Liquid Hand Soap With Piper Aduncum Leaf Extract. (PDF) Antibacterial potential of liquid hand soap with Piper aduncum leaf extract | Melbert Sepe - Academia.edu. Retrieved March 1, 2023, from https://www.academia.edu/39664996/Antibacterial_potential_of _liquid_hand_soap_with_Piper_aduncum_leaf_extract.
[6]	Hastuti, U. S., Ummah, Y. R., & Khasanah, H. N. (2017). Antifungal activity of Piper aduncum and Peperomia pellucida leaf ethanol extract against Candida albicans. In 2ND INTERNATIONAL CONFERENCE ON COMPOSITE MATERIALS AND MATERIAL ENGINEERING (ICCMME 2017).
[7]	Debnath, S., Babu, M. M., Dega, M. F., Bharathi, K. K., Jyothsna, T., Revathi, D., & Kumar, T. S. (2011). Formulation and Evaluation of Liquid Soap Containing Herbal Antimicrobial Agent. Research Journal of Pharmacognosy and Phytochemistry, 3(5), 225-231.
[8]	How to teach your kids handwashing. (n.d.). UNICEF Parenting. https://www.unicef.org/parenting/health/how-to-teach-your-kids-handwashing?fbclid=IwAR2Oui2MyFE1zDgcC8k5pvjqwIgeFP9xWGjgiz3DQ8UTkBAkVPSJnlyuj6E.
[9]	Blaak, J., Keller, D., Simon, I. W., Schleißinger, M., Schürer, N. Y., & Staib, P. (2018). Consumer Panel Size in Sensory Cosmetic Product Evaluation: A Pilot Study from a Statistical Point of View. Journal of Cosmetics, Dermatological Sciences and Applications, 08(03), 97-109.
[10]	Burton, Cobb, Donachie, Judah, Curtis, & Schmidt. (2012, January 1). The Effect of Handwashing With Water or Soap on Bacterial Contamination of Hands - PubMed. PubMed. Retrieved May 7, 2023.
[11]	Ayad, A. C., Buerano, K. N., Siregar Sormin, S. A., Carmela, C., Malabat, M., Jael, S., Lea, D., & Lucero, J. (2017, October 30). Antibacterial Efficiency of Pomelo Peel Extract on Various Concentrations Against Selected Microorganisms | Abstract Proceedings International Scholars Conference. Antibacterial Efficiency of Pomelo Peel Extract on Various Concentrations against Selected Microorganisms | Abstract Proceedings International Scholars Conference. Retrieved February 24, 2023, from https://jurnal.unai.edu/index.php/isc/article/view/1560#: ~:text=aureus%20in%20the%2075%25%20concentration,aureus.
[12]	Rahman, M. S., Juyena, N. S., & Moon, H. (2018). Citrus maxima (pomelo) peel: A potential source of bioactive compounds. Journal of Food Biochemistry, 42(6), e12603.
[13]	Paloma, L. V. (2022). LEMONGRASS (CYMBOPOGON CITRATUS) INTO LAUNDRY DETERGENT PRODUCT. International Journal of Research Publications, 106(1).
[14]	Greenaway, R., Ormandy, K., Fellows, C., & Hollowood, T. A. (2018). Impact of hand sanitizer format (gel/foam/liquid) and dose amount on its sensory properties and acceptability for improving hand hygiene compliance. Journal of Hospital Infection, 100(2), 195-201.