Isolation and Identification of Cellulose Degrading Bacteria from Mangrove Soil of Mahanadi River Delta and Their Cellulase Production Ability

B.C. Behera¹, S. Parida², S.K. Dutta³ and H.N. Thatoi^4,

¹Department of Biotechnology, North Orissa University, Baripada, India

²Department of Biotechnology, MITS School of Biotechnology, Bhubaneswar, India

³Centre for Ecological Sciences, Indian Institute of Science, Bangalore, India

⁴Department of Biotechnology, College of Engineering and Technology (BPUT), Bhubaneswar, India

Cite this paper:
B.C. Behera, S. Parida, S.K. Dutta and H.N. Thatoi. Isolation and Identification of Cellulose Degrading Bacteria from Mangrove Soil of Mahanadi River Delta and Their Cellulase Production Ability. American Journal of Microbiological Research. 2014; 2(1):41-46. doi: 10.12691/ajmr-2-1-6

Abstract

This study was conducted to isolate cellulose degrading bacteria from mangrove soil of Mahanadi river delta, Odisha, India and also to evaluate their cellulase production ability. Results showed that in total fifteen cellulose degrading bacteria were isolated based on their halo zone formation on congored agar medium. Their maximum carboxymethylcellulose hydrolysis capacities (HC value) were ranged from 1.18 to 2.5 cm. A CMCase test of these fifteen isolates showed that their enzyme activity ranged from 2.471 to 98.253 U/ml/min. From the morphological, aand biochemical characterisation, the isolates were identified as Micrococcus spp., Bacillus spp., Pseudomonas spp., Xanthomonas spp. and Brucella spp.

Keywords:
mangrovecellulose degrading bacteriacellulase biochemical characterisation

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