Production of Biosurfactants and Hydrolytic Enzymes by Bacillus-Genus Bacteria Isolated from Mbala Pinda, Traditional Food, Republic of Congo

Priscillia Renée Nguimbi-Tsati¹, Christian Aimé Kayath^{1, 2}, Nicaise Saturnin Mokemiabeka¹, Tarcis Baloki Ngoulou¹, Thantique Moutali Lingouangou^{1, 3}, Yannich Okouakoua^{1, 2} and Gabriel Ahombo^{1, 3,}

¹Laboratoire de Biologie Cellulaire et Moléculaire, Faculté des Sciences et Techniques, Université Marien Ngouabi, Brazzaville, République du Congo

²Institut National de Recherche en Sciences Exactes et Naturelles (IRSEN), Avenue de l’Auberge Gascogne, Brazzaville, République du Congo, P.O Box 2400

³Unité de Microbiologie moléculaire et de Bioinformatique, Faculté des Sciences et Techniques, Université Marien Ngouabi, Brazzaville, Congo

Cite this paper:
Priscillia Renée Nguimbi-Tsati, Christian Aimé Kayath, Nicaise Saturnin Mokemiabeka, Tarcis Baloki Ngoulou, Thantique Moutali Lingouangou, Yannich Okouakoua and Gabriel Ahombo. Production of Biosurfactants and Hydrolytic Enzymes by Bacillus-Genus Bacteria Isolated from Mbala Pinda, Traditional Food, Republic of Congo. American Journal of Microbiological Research. 2022; 10(3):76-83. doi: 10.12691/ajmr-10-3-1

Abstract

Mbala pinda is one of the traditional foods consumed in Congo. In this study, the objective of which was to analyze the production of biosurfactants and that of hydrolytic enzymes from five strains of the Bacillus genus isolated from Mbala pinda and identified by gene analysis and 16S rRNA. These five strains already present in GenBank are the following:IM1=Bacillus safensis MPRN8 (MT107116), IL1=Bacillus megaterium MPRN5 (MT107117), IN1=Bacillus amyloliquefaciens MPRN2 (MT107118), ID1=Bacillus subtilis MPRN7 (MT107119), IMa1=Bacillus velezensis MPRN1 (MT107120). These bacteria are cultured, the cultures are then centrifuged, the supernatant obtained is used to demonstrate the production of biosurfactants and hydrolytic enzymes. Finally, an evaluation of the production of biosurfactants and that of hydrolytic enzymes is made, the correlations for each strain are established between the production of biosurfactants and that of hydrolytic enzymes. The results obtained showed that all five strains produce revealable bioisurfactants from the supernatant of the cultures and both in the presence of gasoline and diesel. The emulsification indexes for the five strains tested are greater than 60% for gasoline and greater than 50% for diesel. The five strains produce proteolytic enzymes with higher productions for IN1 and ID1. The Five strains produce cellulolytic enzymes, IN1 and ID1 are the best producers in our working conditions. The five strains also produce amylolytic enzymes in significant proportions in comparison with the control strain, but here it is IMa1 the main producer and also IM1 and IN1 which also produce significant quantities of these enzymes. The PCA analytic correlations for the production of hydrolytic enzymes according to the different strains and their growth show that each strain has its own characteristics, but all produce these enzymes.

Keywords:
mbala pinda Biosurfactants hydrolytic enzymes traditional food

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References:

[1]	Kobawila et al., 2003. Handbook of indigenous Food Involving Alkaline Fermentation, page 141.
[2]	Kayath, C. A., Nguimbi E., Goma Tchimbakala, J., Mamonékéné, V., Lebonguy, A. A. and Ahombo, G., 2016. Towards the understanding of fermented food Biotechnology in Congo Brazzaville. Advance Journal of Food Science and Technology 12(11), 593-602.
[3]	Nicaise Saturnin Mokemiabeka, Christian Aimé Kayath1, Etienne Nguimbi, Augustin Aimé Lébonguy, Alfred Guy Macaire Eboungabeka, Ricardo De Mendosa, Simon Kéléké, Simon Charles Kobawila1 and Anne Botteaux (2006) Microbiological and biochemical assessment of crushed red pepper from Capsicum frutescens preserved in jars and manufactured in local markets in Republic of Congo, International Journal of Biotechnology Research Vol. 4(1), pp. 001-010.
[4]	Didine Priscilla Moutou-Tchitoula, Etienne Nguimbi, Stéphanie Giusti-Miller, Philippe Mora, Simon Charles Kobawila, Edouard Miambi*(2018). Assessment of dominant bacterial strains isolated from Ntoba mbodi, an indigenous African alkaline-fermented food, and their potential enzyme activities) African Journal of Microbiology Research, Vol. 12(32), pp. 779-787, 28.
[5]	Nguimbi E., Ahombo G., Moyen R., Ampa R., Vouidibio A., Ontsira E. N., Kobawila S. C., Louembe D.(2014) « Optimization of growth, fibrinolytic enzyme production and PCR amplification of encoding fibrinolytic enzyme gene in bacillus amyloliquefaciens isolated from ntoba mbodi at Brazzaville ». International Journal of Science and Research; 3 (358): 2799-2803.
[6]	Alain Brice Mbozo Vouidibio, Christian Aimé Kayath, Etienne Nguimbi, Augustin Aimé Lebonguy and Simon Charles Kobawila (2017). Potential Spore-Forming Probiotics Isolated from Ntoba mbodi, Alkaline Fermented Leaves of Cassava from the Republic of the Congo. International Journal of Science and Research. Volume 6 Issue 1. 2319-7064.
[7]	Sharma K. M., Kumar R., Panwar S., Kumar A. 2017. Microbial alkaline proteases: Optimization of production parameters and their properties. Journal of Genetic Engineering and Biotechnology. 15:115-126.
[8]	Reddy NS, Nimmagada A, Sambasiva Rac RS. (2003). An over view of the microbial alpha amylase minireview. Afri J Biotechnol. 2: 645-48.
[9]	Saeeda Bano, Shah Ali Ul Qader, Afsheen Aman, Muhammad Noman Syed and Abid Azhar. (2011). Purification and Characterization of Novel α-Amylase from Bacillus subtilis KIBGE HAS. AAPS PharmSciTech, Vol. 12, No. 1.
[10]	Lee, S. M. and Y. M. Koo (2001). Pilot-scale production of cellulose using Trichoderma reesei Rut C-30 in fed-batch mode. J. Microbiol. Biotechnol. 11: 229-233.
[11]	Juliana M.Luna, Raquel D. Rufino, Leonie A. Sarubbo, Galba Maria, Campos-Takaki. (2013). Characterisation, surface properties and biological activity of a biosurfactant produced from industrial waste by Candida sphaerica UCP0995 for application in the petroleum industry. Colloids and Surfaces B: Biointerfaces. Volume 102, Pages 202-209.
[12]	David G. Cooper* and Beena G. Goldenberg (1987). Surface-Active Agents from Two Bacillus Species. Appl Environ Microbiol. 53(2): 224-229. 1987.
[13]	Acourene, S. and Ammouche (2012A). Optimization of ethanol, citric acid and α-amylase production from date wastes by strains of Saccharomyces cerevisiae, Aspergillus niger and Candida guilliermondii. Journal of Industrial Microbiology and Biotechnology, 39, 759-766.
[14]	Nguimbi Etienne, Wu ZhiRong (2002). Bacterial growth and enzyme production Conditions, Purification and characterisation of a new Fibrinolytic enzyme. Biotechnology (in Chinese) Vol 12, No 2.
[15]	Soloka Mabika Armel Faly, Rachel Moyen, Etienne Nguimbi*, Gabriel Ahombo, Raoul Ampa, Aimé Christian Kayath, Alain Vouidibio, Cyr Jonas Morabandza and Simon Charles Kobawila (2017). Production, Partial Purification and Based SDS-PAGE profiles of Caseinolytic Enzyme in two Bacillus strains isolated from fermented cassava leaves “ntoba mbodi” in congo Brazzaville. Journal of pure and applied microbiology, vol. 11(1), p. 77-86.
[16]	Anjana Sharma*, Arunima Sharma and L. Shivlata (2015). Optimization of Medium Components for Enhanced Production of Extracellular Fibrinolytic Protease from Citrobacter braakiiInt. J.Curr.Microbiol.App.Sci4 (7): 248-259.
[17]	Ponnuswamy Vijayaraghavan and Samuel Gnana Prakash Vincent (2014). Statistical Optimization of Fibrinolytic Enzyme Production Using Agroresidues by Bacillus cereus IND1 and Its Thrombolytic Activity In Vitro Hindawi Publishing Corporation BioMed Research International Volume, 11 pages.
[18]	De Vos, P., Garrity, G.M., Jones, D., Krieg, N.R., Ludwig, W., Rainey, F.A., Schleifer, K.-H. & Whitman, W.B. 2009. Volume Three. The Firmicutes. In G.M. Garrity (ed.), Bergey's Manual of Systematic Bacteriology, Second Edition. Springer-Verlag, New York: [I]-XXVI, 1-1422.
[19]	Ngo-Itsouhou, Etienne Nguimbi, Rachel Moyen, Armel Faly Soloka Mabika Harnessing Biological Activities in Soil-Bacillus Strains to Promote the Discovery of New Bioactive Compounds. Journal of Applied & Environmental Microbiology, 2020, Vol. 8, No. 1, 32-38, ISSN (Print) :2373-6747, ISSN (Online): 2373-6712.
[20]	Schallmey, M., Singh, A., and Ward, O. P., 2004. Développements in the use of Bacillus species for industrial production. Can. 1. Microbial, 50: 1-17.
[21]	Kasing Apun, Bor Chyan Jong, Mohd. Azib Salleh (2000). Screening and isolation of a cellulolytic and amylolytic Bacillus from sago pith waste. The Journal of General and Applied Microbiology 46(5), p. 263-267.
[22]	Kaya-Ongoto, M.D., Kayath, C.A., Mbozo, A.B.V., Mitoko, G.M., Wilson, S.P.E., Kinavouidi, D.J.K. and Nguimbi, E. (2020) Prime Enzymatic Exocellular Background of Lysinibacillus louembei. Advances in Microbiology, 10, 95-109.
[23]	Ouoba, L., Vouidibio Mbozo, A.B., Thorsen, L., Anyogu, A., Nielsen, D.S., Kobawi-la, S.C., et al. (2015). Lysinibacillus louembei sp. nov., a Spore-Forming Bacterium Isolated from Ntoba Mbodi, Alkaline Fermented Leaves of Cassava from the Republic of the Congo. International Journal of Systematic and Evolutionary Microbiology, 65, 4256-4262.
[24]	Srivastava, R.A.K. and Baruah, J.N. (1986) Culture conditions for production of thermostable amylase by Bacillus stearothermophilus. Applied and Environmental Microbiology, 52, 179-184.
[25]	Thantique Moutali Lingouangou, Etienne Nguimbi, Saturnin Nicaise Mokemiabeka, Ngo-Itsouhou, Isaac Onyankouang, and Faly Armel Soloka Mabika. (2022), “Antibiotic Resistance and Antibacterial Activity in Four Strains Isolated from Soils in Brazzaville Republic of Congo.” Journal of Applied & Environmental Microbiology, vol. 10, no. 1 1-8.
[26]	Dai Kitamoto, Hiroko Isoda, Tadaatsu Nakahara (2002). Functions and potential applications of glycolipid biosurfactants--from energy-saving materials to gene delivery carriers. J Biosci Bioeng 2002; 94(3): 187-201.
[27]	S. M. M. Dastgheib, M. A. Amoozegar Æ E. Elahi, S. Asad Æ I. M. Banat (2008) Bioemulsifier production by a halothermophilic Bacillus strain with potential applications in microbially enhanced oil recovery. Biotechnol Lett. 30: 263-270.
[28]	Adegoke Isiaka Adetunji1 and Ademola Olufolahan Olaniran. Production and characterization of bioemulsifiers from Acinetobacter strains isolated from lipid-rich wastewater, Biotech (2019) 9: 151.