Characterization of Isolated Bacteria from Soils in the Likouala Peat Bog Area (Republic of Congo)

Morabandza Cyr Jonas^{1, 2,} , Gatsé Elgie Viennechie^{1, 2}, Mboukou Kimbatsa Irène Marie Cecile^{1, 2}, Onyankouang Isaac Samuel^{1, 2}, Ifo Suspence Averti³ and Nguimbi Etienne^{1, 2}

¹Laboratory of Microbiology, Infectiology and Immunology, ENS-UMNG, B.P 69 Brazzaville

²Laboratory of Cellular and Molecular Biology, FST-UMNG, B.P 69 Brazzaville

³Laboratory of Remote Sensing and Forest Ecology, ENS-UMNG, B.P 69 Brazzaville

Cite this paper:
Morabandza Cyr Jonas, Gatsé Elgie Viennechie, Mboukou Kimbatsa Irène Marie Cecile, Onyankouang Isaac Samuel, Ifo Suspence Averti and Nguimbi Etienne. Characterization of Isolated Bacteria from Soils in the Likouala Peat Bog Area (Republic of Congo). American Journal of Microbiological Research. 2022; 10(2):59-70. doi: 10.12691/ajmr-10-2-3

Abstract

The aims of this study were to characterized the bacteria isolated from the soils of Likouala peat bog area in Congo-Brazzaville. Three (03) composite samples were made from a total of 9 soil samples collected. 1g of soil from each composite sample was cultured on PCA, Mossel and EMB media for counting the total flora. Isolation on Mossel medium and identification of isolates was based on cultural, morphological and biochemical characters by conventional techniques. The method of Petri dishes containing the substrate relating to the search for proteolytic and amylolytic activities was used. The results showed total bacterial loads of 9.50x10⁴; 7.31x10⁴ and 6.39x10⁴ CFU/g on PCA medium; 5.81x10⁴; 5.64x10⁴ and 8.56x10³ CFU/g on Mossel medium versus 2.77x10² CFU/g; 5.1x10² and 1.16x10² CFU/g on EMB medium respectively for samples 1, 2 and 3. The respective total anaerobic mesophilic flora of 3.08x10⁴; 2.93x10⁴ and 3.03x10³ CFU/g on PCA medium; 2.48x10³; 4.83x10³ and 3.32x10³ CFU/g on Mossel medium for samples 1, 2 and 3. The optical densities of the aerobic isolates were between 0.634 and 1 and the enzyme production showed diameters of translucent halos between 1 to 3.2 cm for the proteolytic enzymes and between 1.2 to 2.7 cm for the amylolytic enzymes. The evolution of enzyme production is a function of time and of the isolate. The surface part of the soils of the Likouala peat bog area is rich in aerobic and anaerobic bacteria.

Keywords:
bacteria peatland amylase soil protease

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