Decolorization of Reactive Black 5 by Yarrowia lipolytica NBRC 1658

Y. Doruk ARACAGÖK^, and Nilüfer CİHANGİR

Cite this paper:
Y. Doruk ARACAGÖK and Nilüfer CİHANGİR. Decolorization of Reactive Black 5 by Yarrowia lipolytica NBRC 1658. American Journal of Microbiological Research. 2013; 1(2):16-20. doi: 10.12691/ajmr-1-2-1

Abstract

Yarrowia lipolytica NBRC 1658 could decolorize Reactive Black 5 effectively through biodegradation. The optimum conditions such as initial pH, glucose concentration, nitrogen concentration and initial dye concentration were determined. Correlations between decolorization and laccase and manganese peroxidase activities were investigated. Neither laccase nor manganese peroxidase (MnP) activities were determined in culture mediums. Y. lipolytica could decolorize 97% percentage of 50mg l^-1 RB5 dye within 24 hours and could tolerate up to 300 mg l^-1 of dye. It was found that decolorization occurred during the exponential growth phase. Aerobic batch conditions with 5g l^-1 glucose and 1g l^-1 ammonium sulphate at pH 7 were considered to be the optimum decolorizing conditions.

Keywords:
biodegradation decolorization Azo dyes Reactive Black 5 Yarrowia lipolytica

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References:

[1]	Saratale RG, Saratale GD, Chang JS et al. Bacterial decolorization and degradation of azo dyes: A review. J Taiwan Inst Chem E 42: 138-157, 2011.
[2]	Soares GMB, Amorim MTP, Hrdina R et al. Studies on the biotransformation of novel diazo dyes by laccase. Process Biochem 37: 581-587, 2002.
[3]	Yang, Q, Yediler A, Yang M et al. Decolorization of an azo dye, reactive black 5 and MnP production by yeast isolate: Debaryomyces polymorphus. Biochem Eng J 24: 249-253, 2005.
[4]	Murugesan K, Dhamija A, Nam IH et al. Decolourization of reactive black 5 by laccase: Optimization by response surface methodology. Dyes Pigments 75: 176-184, 2007.
[5]	Kaushik P, Malik A. Fungal dye decolourization: Recent advances and future potential. Environ Int 35: 127-141, 2009.
[6]	Patel R, Suresh S. Kinetic and equilibrium studies on the biosorption of reactive black 5 dye by Aspergillus foetidus. Bioresource Technol 99: 51-58, 2008.
[7]	Wang HJQ, Su X, Zheng W et al. Bacterial decolorization and degradation of the reactive dye reactive red 180 by Citrobacter sp. Ck3. Int Biodeter Biodegr 63: 395-399, 2009.
[8]	Selvam K, Swaminathan K, Chae KS. Decolourization of azo dyes and a dye industry effluent by a white rot fungus Thelephora sp. Bioresource Technol 88: 115-119, 2003.
[9]	Mazmanci MA, Ünyayar A. Decolourisation of reactive black 5 by Funalia trogii immobilised on Luffa cylindrica sponge. Process Biochem 40: 337-342, 2005.
[10]	Couto SR, Rosales E, Sanromán MA. Decolourization of synthetic dyes by Trametes hirsuta in expanded-bed reactors. Chemosphere 62: 1558-1563, 2006.
[11]	Mechichi T, Mhiri N, Sayadi S. Remazol brilliant blue decolourization by the laccase from Trametes trogii. Chemosphere 64: 998-1005, 2006.
[12]	Hailei W, Ping L, Min P et al. Rapid decolourization of azo dyes by a new isolated higher manganese peroxidase producer: Phanerochaete sp. Hsd. Biochem Eng J 46: 327-33, 2009.
[13]	Maalej-Kammoun M, Zouari-Mechichi H, Belbahri L et al. Malachite green decolourization and detoxification by the laccase from a newly isolated strain of Trametes sp. Int Biodeter Biodegr 63: 600-606, 2009.
[14]	Singh S, Pakshirajan K. Enzyme activities and decolourization of single and mixed azo dyes by the white-rot fungus Phanerochaete chrysosporium. Int Biodeter Biodegr 64: 146-150, 2010.
[15]	Lucas MS, Amaral C, Sampaio A et al. Biodegradation of the diazo dye reactive black 5 by a wild isolate of Candida oleophila. Enzyme Microb Tech 39: 51-55, 2006.
[16]	Jadhav JP, Parshetti GK, Kalme SD. Decolourization of azo dye methyl red by Saccharomyces cerevisiae MTCC 463. Chemosphere 68: 394-400, 2007.
[17]	Jain MR, Zinjarde SS, Deobagkar DD et al. 2,4,6-Trinitrotoluene transformation by a tropical marine yeast, Yarrowia lipolytica NCIM 3589. Mar Pollut Bull 49: 783-788, 2004.
[18]	Murphy GL, Perry JJ, Assimilation of chlorinated alkanes by hydrocarbon utilizing fungi. J. Bacteriol 160: 1171-1174, 1984.
[19]	Barth G, Gaillardin C. Yarrowia lipolytica. In: Wolf, W.K. (Ed.), Nonconventional Yeasts in Biotechnology, vol. 1. Springer-Verlag, Berlin, Germany, pp. 313-388, 1996.
[20]	De Felice B, Carfagna M, Pontecorvo G. Degradation ofwaste waters from olive oil mills by Yarrowia lipolytica ATCC20255 and Pseudomonas putida. Acta Biotechnol 17: 231-239, 1997.
[21]	Oh YS, Maeng J, Kim SJ. Use of microorganism-immobilized polyurethane foams to absorb and degrade oil on water surface. Appl. Microbiol Biotechnol 54: 418-423, 2000.
[22]	Schmitz C, Goebel I, Wagner S et al. Competition between n-alkane-assimilating yeasts and bacteria during colonization of sandy soil microcosms. Appl Microbiol. Biotechnol 54: 126-132, 2000.
[23]	Mauersberger S, Wang H, GaillardinC et al. Insertional mutagenesis in the n-alkane-assimilating yeast Yarrowia lipolytica: generation of tagged mutations in genesinvolved in hydrophobic substrate utilization. J. Bacteriol 183: 5102-5109, 2001.
[24]	Bourbonnais R, Paice MG. Oxidation of non-phenolic substrates: An expanded role for laccase in lignin biodegradation. FEBS Lett 267: 99-102, 1990.
[25]	Kuwahara M, Glenn JK, Morgan MA et al. Separation and characterization of two extracelluar H2O2-dependent oxidases from ligninolytic cultures of Phanerochaete chrysosporium. FEBS Lett 169: 247-250, 1984.
[26]	Wang X, Cheng X, Sun D et al. Biodecolorization and partial mineralization of reactive black 5 by a strain of Rhodopseudomonas palustris. J Environ Sci 20: 1218-1225, 2008.
[27]	Yang Q, Tao L, Yang M et al. Effects of glucose on the decolorization of reactive black 5 by yeast isolates. J Environ Sci 20: 105-108, 2008.
[28]	Deive FJ, Domínguez A, Barrio T et al. Decolorization of dye reactive black 5 by newly isolated thermophilic microorganisms from geothermal sites in Galicia (Spain). J Hazard Mater 182: 735-742, 2010.
[29]	Khelifi E, Ayed L, Bouallagui H et al. Effect of nitrogen and carbon sources on indigo and congo red decolourization by Aspergillus alliaceus strain 121c. J Hazard Mater 163: 1056-1062, 2009.
[30]	Eelko GS, Natal AW, Riel V et al. The role of ammonia metabolism in nitrogen catabolite repression inSaccharomyces cerevisiae.FEMS MICROBIOL REV 24: 67-83, 2000.
[31]	Hatvani N, Mécs I. Effect of the nutrient composition on dye decolorisation and extracellular enzyme production by Lentinus edodes on solid medium. Enzyme Microb Tech 30: 381-386, 2002.
[32]	Dias A, Lucas M, Sampaio A et al. Decolorization of azo dyes by yeasts biodegradation of azo dyes. Springer. Berlin, pp. 183-193, 2010.
[33]	Patricia A, Ramalho M, Cardoso H M, Cavaco-Pauloi A, Teresa M T, Characterization of azo reduction activity in a novel ascomyecete yeast strain. Appl. Environ. Microbiol. 70: 2279-2288.