Twitching Motility, Biofilm Communities in Cephalosporin Resistant Proteus spp and the Best in vitro Amoxicillin Susceptibility to Isolates

Aktar Uzzaman Chouduri^1, and Abdul Wadud¹

¹Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh

Cite this paper:
Aktar Uzzaman Chouduri and Abdul Wadud. Twitching Motility, Biofilm Communities in Cephalosporin Resistant Proteus spp and the Best in vitro Amoxicillin Susceptibility to Isolates. American Journal of Microbiological Research. 2014; 2(1):8-15. doi: 10.12691/ajmr-2-1-2

Abstract

Proteus strains isolated from tap water are assessed for their twitching motility and biofilm formation on urethral catheter strips of two selected brands, Protos and Huaian, since these strains are the common cause of catheter associated urinary tract infection (CAUTI). Neither the strains of high swarming motility nor of high antimicrobial resistance enabled to twitch over the catheter strips. All of high antibiotic resistant strains were found to form biofilm on PVC strip, few strains on Huaian catheter strip and one strain (Pv) on both catheter strips. Based on biofilm quantification study, the urethral catheter from Protos brand was relatively safer than that of Huaian brand to prevent CAUTI. Moreover, Proteus isolates were strong resistant to cotrimoxazole (folate synthesis inhibitor) and cephalosporins (cell wall synthesis inhibitor). Underlying the β-lactam group, strong cephalosporin resistance and amoxicillin susceptibility in isolates indicated that the isolates were non β-lactamase producer. Tested antibiotics underlying the protein and DNA synthesis inhibitors were satisfactorily effective for the inhibition of Proteus isolates. The phenolic acid, –C₆H₄OH, present in the chemical formula of amoxicillin plays a crucial role in cell wall synthesis of Proteus isolates allowing more drugs to cell wall via porins.

Keywords:
Proteus urethral catheter biofilm cephalosporin resistance amoxicillin sensitivity

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