Assessment of Immunodiagnostic and Molecular Detection of Hepatitis B among Pregnant Women (Spontaneous Recurrent Miscarriage and with No History of Miscarriage) - in Gezira State -2018

Maab Abdalbaset^1, , Osama Mohammed Khair² and Nadir Abuzeid³

¹Medical Microbiology Department, faculty of Medical Laboratory Science, Alneelain University

²Public Central Laboratory

³Medical Microbiology Department, Faculty of Medical Laboratory Science, Omdurman Islamic University

Cite this paper:
Maab Abdalbaset, Osama Mohammed Khair and Nadir Abuzeid. Assessment of Immunodiagnostic and Molecular Detection of Hepatitis B among Pregnant Women (Spontaneous Recurrent Miscarriage and with No History of Miscarriage) - in Gezira State -2018. American Journal of Microbiological Research. 2020; 8(2):42-47. doi: 10.12691/ajmr-8-2-1

Abstract

Background: Infection with hepatitis B virus are often occult, as positive result but no symptoms, so this will cause increase of infection without knowing. Pregnant women suffer always form complications regarding the gestation itself or related issues cause miscarriage, which may be devastated if it became adaily routine making history for coming future. Therefore, this study aimed to guage prevalence of infection with hepatitis B virus by different methods so as to make sure of the techniques regarding the result and to ascertain how the virus has been spread among recurrent miscarriage history women in Gezira state-Sudan. Material and method: Whole blood samples were obtained from each participant, serum was separated for detection of antibodies IgG and IgM of HBV via ICT and EISA, and EDTA added blood later used for DNA extraction so as to perform PCR genotype for HBV. Laboratory work was conducted at Alneelain University-faculty of medical laboratory science-microbiology department. Data analyzed using SPSS version 21. Result: Ninety women were enrolled in each arm of study. All samples enrolled during this study were provides a positive 4 (4.4%) for IgG antibodies for HbV by ELISA. However, There have been abig difference in miscarriage and non miscarriage serum IgM and pcr -positivity for HbV (0 (0%)%) vs 90 (100%) and borderline4(4.4%). by ELISA. In logistic multivairte analysis of the predictors for miscarriage (OR=000, 95%Cl=0.000-0.000, P value= 0.993) IgM sero-negative for miscarriage. Other significant risk factors include microcytic hypochromic anemia, vaginal bleeding, pre-eclampsia and case history. Conclusion: In the current study HBV Immunodiagnostic and molecular negatively isn’t related to miscarriage. . hould be introduced for pregnant women during this setting.further research is required.

Keywords:
miscarriage enzyme linked immuno sorbent assay polymerase chain reaction

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