Frequency of Estrogen Receptor-1 (ESR-1) Gene Polymorphism (PvuII and XbaI) in Patients with Coronary Artery Disease

Reham A. Mariah^{1, 2}, Hussam Baghdadi¹, Kamal El-din Ahmed³, Naglaa Mostafa⁴, Mongi Mohamed Ahmed Ayat^{1, 5}, Tayseer Nansour^{6, 7}, Manal Mohamed Helmy Nabo⁸, Salah Mohamed El Sayed^{1, 9,} and Nassar Ayoub Abdellatif Omar¹⁰

¹Department of Clinical Biochemistry and Molecular Medicine, Taibah Faculty of Medicine, Taibah University, Al-Madinah Al-Munawwarah, Saudi Arabia

²Department of Medical Biochemistry, Tanta Faculty of medicine, Tanta University, Egypt

³Department of Cardiology, Tanta Faculty of medicine, Tanta University, Egypt

⁴Department of Clinical and Chemical Pathology, National Cancer Institute, Cairo University, Egypt

⁵Department of Clinical Pathology, Faculty of Medicine, 6th October University, Cairo, Egypt

⁶Department of Medical Education, Suez Canal Faculty of Medicine, Suez Canal University, Egypt

⁷Department of Medical Education, Taibah Faculty of Medicine, Taibah University, Al-Madinah Al-Munawwarah, Saudi Arabia

⁸Division of Pediatric cardiology, Department of Pediatrics, Maternity and Children Hospital, King Abdullah Medical City, Al-Madinah Al-Munawwarah, Kingdom of Saudi Arabia

⁹Department of Medical Biochemistry, Sohag Faculty of Medicine, Sohag University, Egypt

¹⁰Department of Anatomy, Faculty of Medicine, Sohag University, Egypt

Cite this paper:
Reham A. Mariah, Hussam Baghdadi, Kamal El-din Ahmed, Naglaa Mostafa, Mongi Mohamed Ahmed Ayat, Tayseer Nansour, Manal Mohamed Helmy Nabo, Salah Mohamed El Sayed and Nassar Ayoub Abdellatif Omar. Frequency of Estrogen Receptor-1 (ESR-1) Gene Polymorphism (PvuII and XbaI) in Patients with Coronary Artery Disease. American Journal of Medical and Biological Research. 2016; 4(1):5-9. doi: 10.12691/ajmbr-4-1-2

Abstract

Sex hormones might be viewed as biomarkers for cardiovascular health status, as well as protective agents against heart diseases. Coronary artery disease (CAD) is the most common disease in humans and has a complex etiology. In this study, we aimed to investigate the association of CAD with ESR1 PvuII and XbaI gene mutation frequencies and to see if it's important as a genetic risk factor and the susceptibility for CAD in Tanta region, Egypt. By polymerase chain reaction with restriction fragment length polymorphism (PCR- RFLP), we determined the frequency of the ESR1 gene polymorphisms in 110 healthy and 100 CAD sample. Results revealed that there were no significant differences between CAD patients and the control group as regard the frequency AA, AG and GG of XbaI genotype. PvuII genotype frequencies were TT, TC and CC with no significant value. Regarding allele frequencies of PvuII and XbaI polymorphism, they were not statistically important. There was no significant difference among all studied subjects regarding sex, age, menopausal status, cardiac complications and lipid profile, but there was highly significant differences regarding the body mass index. In conclusion, estrogen receptor alpha gene polymorphism Pvu II and XbaI site are not associated with the coronary artery disease.

Keywords:
coronary artery disease estrogen receptor-1 gene allele frequency PCR-RFLP

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