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American Journal of Medical and Biological Research. 2016, 4(1), 1-4
DOI: 10.12691/ajmbr-4-1-1
Open AccessArticle

The Effect of Fruit Extracts of Black Pepper on the Fertility Potential of Male Albino Mice

Sutyarso1, Muhartono2 and M. Kanedi3,

1Department of of Biology, Faculty of Mathematics and Sciences, University of Lampung, Indonesia

2Department of Anatomical Pathology, Faculty of Medicine, University of Lampung, Indonesia

3Department of Biology, Faculty of Mathematics and Sciences, University of Lampung, Indonesia

Pub. Date: February 24, 2016

Cite this paper:
Sutyarso, Muhartono and M. Kanedi. The Effect of Fruit Extracts of Black Pepper on the Fertility Potential of Male Albino Mice. American Journal of Medical and Biological Research. 2016; 4(1):1-4. doi: 10.12691/ajmbr-4-1-1


This study aimed to determine the effects of black pepper fruit extract on serum testosterone status and fertility potential in male subjects. Twenty seven healthy male mice, four months old, weighing between 25-30 g, were used as experimental animals and grouped into three. The first group was given pellets that do not contain black pepper extract as the control. The second and third groups, respectively were treated with pellets containing water extract and ethanolic extract. Pellets given ad libitum every day for 90 days. Serum testosterone measured by radioimmunoassay (RIA) techniques. Left testis of mice removed, weighed and dissected. Cauda epididymis was separated from testis and squeezed to determine epididymal sperm concentration, motility, and morphology. To determine the diameter of the seminiferous tubules, the number of spermatocytes and spermatids, testes were fixed in Bouin solution, processed and cut at a thickness of 5 um, stained with hematoxylin and eosin and examined under a light microscope at 400x magnification. When compared with control group, male mice treated with fruit extract of black pepper showed significant increase in the serum testosterone level, epididymal sperm concentration, spermatocyte counts, spermatid counts, and the weight of epididymis tubules. As the conclusion, fruit extract of black pepper has a positive effect on androgenic hormone level and fertility potential in male mice.

piper nigrum black pepper testosterone fertility male mice

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[1]  Kpomah E.D., Uwakwe A.A., Abbey B.W., “Aphrodisiac studies of diherbal mixture of Zanthoxylum leprieurii Guill. And Perr. and Piper guineense Schumach. and Thonn. on male wistar rats,” Global J. Res. Med. Plants Indigen. Med. 1, 381-390. Sep. 2012.
[2]  Rahmawati N. and Bachri M.S., “The aphrodisiac effect and toxicity of combination Piper retrofractum L, Centella asiatica and Curcuma domestica infusion,” Health Sci. J. Indonesia. 3, 19-22. Jun.2012.
[3]  Sutyarso, Kanedi M., Rosa E., “Effects of black pepper (Piper nigrum Linn.) extract on sexual drive in male mice,” Research Journal of Medicinal Plant 9 (1): 42-47., 2015.
[4]  D'Cruz S.C. and Mathur P.P., “Effect of piperine on the epididymis of adult male rats,” Asian J. Androl. 7, 363-368. Dec.2005.
[5]  Dalal, P. K. Tripathi,A and Gupta, S. K.., “Vajikarana: Treatment of sexual dysfunctions based on Indian concepts,” Indian J Psychiatry. 55 (Suppl 2): S273-S276. Jan. 2013.
[6]  Ogbuewu I.P., Unamba-Oparah I.C., Odoemenam V.U., Etuk I.F., Okoli I.C., “The potentiality of medicinal plants as the source of new contraceptive principles in males,” North Am. J. Med Sci. 3, 255-263. Jun. 2011.
[7]  Ukoha U.U., Egwu O.A., Dimkpa U., Maduka S.O., Okafor J.I., Ndukwe G.U., Ogonnadi K., “Histological and weight changes in testes of male albino rats fed with diets containing yaji (A Local Meat Sauce),” Int. J. Health Sci. Res. 4 (8), 118-126. 2014.
[8]  Mohammadi F., Nikzad H., Taherian A., Mahabadi J.A., Salehi M., “Effects of herbal medicine on male infertility,” Anatomical Science. 10(4), 3-15. 2013.
[9]  Chanda D., Shanker K., Pal A., Luqman S., Bawankule D.U., Mani D., Darokar M.P., “Safety evaluation of Trikatu, a generic ayurvedic medicine in charles foster rats,” J. Toxicol. Sci. 34, 99-108. 2009.
[10]  Vijayakumar R.S. and Nalini N., “Piperine, an active principle from Piper nigrum, modulates hormonal and apolipoprotein profiles in hyperlipidemic rats,” J. Basic Clin. Physiol. Pharmacol.17, 71-86. 2006.
[11]  Hirata N., Tokunaga M., Naruto S., Iinuma M., Matsuda H., “Testosterone 5α-reductase inhibitory active constituents of Piper nigrum leaf,” Biol. Pharm. Bull. 30, 2402-2405. Aug./ 2007.
[12]  Meghwal M. and Goswami T.K., “Chemical composition, nutritional, medicinal and functional properties of black pepper: A review,” Open Access Scient. Rep. Vol.1. 2012.
[13]  Gromadzka-Ostrowska J., “Effects of dietary fat on androgen secretion and metabolism,” Reproduct. Biol. 6 Suppl 2:13-20. 2006.
[14]  Bouba A.A., Njintang N.Y., Foyet H.S., Scher J., Montet, D., Mbofung C.M.F., “Proximate composition, mineral and vitamin content of some wild plants used as spices in Cameroon,” Food Nutr. Sci. 3, 423-432. Jul.2012.
[15]  Brilla L.R. and Conte V., “Effects of a novel zinc-magnesium formulation on hormones and strength,” J. Exercise Physiologyonline. 3, 26-36. Oct. 2000.
[16]  Nkansah M.A. and Amoako C.O., “Heavy metal content of some common spices available in markets in the Kumasi metropolis of Ghana,” Am. J. Sci. Ind. Res. 1, 158-163. 2010.
[17]  Hamza S., Srinivasan V., Dinesh R., “Nutrient diagnosis of black pepper (Piper nigrum L.) gardens in Kerala and Karnataka,” J. Spices Arom. Crops. 16, 77-81. Sep.2007.
[18]  Kumar P., Yadav B., Yadav S., “Effect of zinc and selenium supplementation on antioxidative status of seminal plasma and testosterone, T and T level in goat blood serum,” J. Applied Anim. Res. 41, 382-386. May. 2013.
[19]  Egwurugwu J.N., Ifedi C.U., Uchefuna R.C., Ezeokafor E.N., Alagwu E.A., “Effects of zinc on male sex hormones and semen quality in rats,” Niger. J. Physiol. Sci. 28, 17-22. Jun.2013.
[20]  Tikkiwal M., Ajmera R.L., Mathur N.K., “Effect of zinc administration on seminal zinc and fertility of oligospermic males,” Indian J Physiol Pharmacol. 31(1), 30-4. Jan-Mar.1987.
[21]  Yamaguchia, S., Miura, C., Kikuchi K.,, Fritzie T. Celino, F.T., Tetsuro Agusa T., Tanabe S.and Miuraa, T., “Zinc is an essential trace element for spermatogenesis” PNAS 106(26): 10859-10864. Jun. 2009.
[22]  McLachlan R.I., Wreford N.G., Meachem S.J., de Kretser D.M., Robertson D.M., “Effects of testosterone on spermatogenic cell populations in the adult rat,” Biology of Reproduction. 51, 945-955.Jun. 1994.
[23]  Nusier M.K, Bataineh H.N., Bataineh Z.M., Daradka H.M., “Effect of frankincense (Boswellia thurifera) on reproductive system in adult male rat,” Journal of Health Science. 53(4), 365-370. May.2007.
[24]  Ruwanpura S.M, McLachlan R.I., Meachem S.J., “Hormonal regulation of male germ cell development,”. Journal of Endocrinology. 205, 117-131. Feb.2010.
[25]  Takihara H, M.J. Cosentino, and A.T. Cockett., “Effect of low-dose androgen and zinc sulfate on sperm motility and seminal zinc levels in infertile men,” Urology, 22(2):160-4. Aug.1983.
[26]  Henderson N.A., Cooke G.M., Robaire B., “Region-specific expression of androgen and growth factor pathway genes in the rat epididymis and the effects of dual 5α-reductase inhibition,” Journal of Endocrinology. 190, 779-791. 2006. Link
[27]  Pundir R.K and Jain P., “Comparative studies on the antimicrobial activity of black pepper (Piper Nigrum) and turmeric (Curcuma Longa) extracts,” IJABPT. 1(2), 491-501. Aug 2010.
[28]  Tahir, A. and Moeen, R., “Comparison of antibacterial activity of water and ethanol extracts of Camellia sinensis (L.) Kuntze against dental caries and detection of antibacterial components,” Journal of Medicinal Plants Research Vol. 5(18), pp. 4504-4510, Sep. 2011.
[29]  Bae, J.Y., Lee, Y.S., Han, S.Y., Jeong, E.J., Lee, M.K., Kong, J.Y., Lee, D.H., Cho, K.J., Lee, H.S. and Ahn, M.J., “A Comparison between Water and Ethanol Extracts of Rumex acetosa for Protective Effects on Gastric Ulcers in Mice” Biomol. Ther. (Seoul), 20(4): 425-430. Jul. 2012.
[30]  Nahak G. and Sahu R.K., Phytochemical evaluation and antioxidant activity of Piper cubeba and Piper nigrum. J. Applied Pharmaceutical Sci. 01 (08), 153-157. Oct. 2011.
[31]  Hakkim, F.L., Girija, S., Kumar, R.S. and Jalaludeen, M.D., “Effect of aqueous and ethanol extracts of Cassia auriculata L. flowers on diabetes using alloxan induced diabetic rats” Int J Diabetes & Metabolism 15: 100-106. Jan.2007.