Optimising a Fusarium solani Biofilm Formation Protocol in Vitro

Marwan Y. Al-Maqtoofi^1,

¹Department of Biology, College of Sciences, University of Basrah, 61004, Iraq

Cite this paper:
Marwan Y. Al-Maqtoofi. Optimising a Fusarium solani Biofilm Formation Protocol in Vitro. American Journal of Infectious Diseases and Microbiology. 2018; 6(2):62-65. doi: 10.12691/ajidm-6-2-5

Abstract

Opportunistic fungi belonging to the Fusarium solani have become increasingly recognised as life-threatening pathogens causing keratitis and disseminated fusariosis among both healthy individuals and patients with haematological malignancies. These infections are associated with biofilm formation on different biotic and abiotic surfaces. Considering, a biofilm is a virulence factor for causing infections, the aim of this study optimising and illustrating a simple, cost-effective and highly reproducible 96 well microtitre-based method for F. solani biofilm formation via using crystal violet stain. The results revealed that the possibility of using either 570nm or 595nm as a wavelength for quantifying fungal biofilm formation. The best time for crystal violet de-staining was 10 min of incubation. This model can be used in-vitro to quantify and understand the virulence factor of fungal biofilm during infections, and for antifungal susceptibility testing.

Keywords:
Biofilm formation Fusarium solani fungi crystal violet 96 well microtitre plates

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