Validity of Cefoxitin Disc Diffusion Test for the Detection of Methicillin-resistant Staphylococcus Aureus

Hafiza Sultana^1, , Jogendra Nath Sarker², Md. Abdullah Yusuf³, Md. Tofael Hossain Bhuiyan⁴, Md. Mostaqimur Rahman¹ and Shirin Tarafder⁵

¹Department of Microbiology, Rangpur Medical College, Rangpur, Bangladesh

²Department of Microbiology, Dinajpur Medical College, Dinajpur, Bangladesh

³Department of Microbiology, National Institute of Neurosciences & Hospital, Dhaka, Bangladesh

⁴Department of Neurosurgery, Rangpur Medical College Hospital, Rangpur, Bangladesh

⁵Department of Microbiology & Immunology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh

Cite this paper:
Hafiza Sultana, Jogendra Nath Sarker, Md. Abdullah Yusuf, Md. Tofael Hossain Bhuiyan, Md. Mostaqimur Rahman and Shirin Tarafder. Validity of Cefoxitin Disc Diffusion Test for the Detection of Methicillin-resistant Staphylococcus Aureus. American Journal of Infectious Diseases and Microbiology. 2018; 6(1):26-29. doi: 10.12691/ajidm-6-1-4

Abstract

Definite detection of MRSA is important for proper treatment. The purpose of the present study was to measure the validity of cefoxitin disc diffusion test for the detection of methicillin-resistant Staphylococcus aureus. This cross-sectional study was carried out in the Department of Microbiology and Immunology at Bangabandhu Sheikh Mujib Medical University, Dhaka from January 2010 to December 2010 for a period of one (01) year. S. aureus isolates were collected from different clinical samples including wound swab, pus, blood, urine, tracheal aspirate, throat swab, aural swab etc. Staphylococcus aureus (S.aureus) were isolated and confirmed by staining, biochemical tests. Routine antimicrobial susceptibility testing was performed cefoxitin discs diffusion test. PCR was performed for detection of the mecA gene for MRSA. Out of the 22 suspected MRSA isolates 19 were mecA positive by PCR and all of them 19(100.0%) were resistant to cefoxitin disc diffusion. The comparison of oxacillin and cefoxitin resistance and presence of mecA gene by PCR showed that out of 22 suspected MRSA isolates 19 were mecA positive by PCR and all the 19 (100.0%) showed resistance to cefoxitin disc diffusion. The sensitivity and specificity of cefoxitin disc diffusion were 100.0% and 100.0% respectively. Cefoxitin disc diffusion test has high sensitivity and specificity for the detection of MRSA.

Keywords:
validity test cefoxitin disc diffusion test methicillin-resistant Staphylococcus aureus

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