Photodynamic Disinfection of Dentures

Angelina Pecheva Vlahova^1, , Christo Kalchev Kissov¹, Elka Vakrilova Popova² and Georgi Rangelov Todorov¹

¹Department of Prosthetic Dental Medicine, Faculty of Dental Medicine, Plovdiv, Bulgaria

²Department of Periodontology and Oral Mucosa Diseases, Faculty of Dental Medicine, Plovdiv, Bulgaria

Cite this paper:
Angelina Pecheva Vlahova, Christo Kalchev Kissov, Elka Vakrilova Popova and Georgi Rangelov Todorov. Photodynamic Disinfection of Dentures. American Journal of Infectious Diseases and Microbiology. 2013; 1(2):34-37. doi: 10.12691/ajidm-1-2-2

Abstract

Photodynamic disinfection is a new method for cleaning dentures of edentulous patients. It is based on the photochemical action of a photoactive dye (photosensitizer), activated by proper light in the presence of oxygen. The mechanism of photosensitization directs the generation of reactive oxygen species that destroy the pathogens. A pilot study of photodynamic disinfection of dentures was carried out. Four different photosensitizers susceptible to red light were used for the investigation. Results showed that photodynamic disinfection is an effective method for inactivation of pathogens on dentures as a part of prevention of cross-contamination in dental office. It also can be used as a part of the therapy of denture stomatitis.

Keywords:
photodynamic disinfection dentures prevention cross-contamination

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References:

[1]	Donnely R, P McCarron, M Tunney. Antifungal photodynamic therapy. Microbiological Research, 163(1), 1-12, 15 Jan 2008
[2]	Kömerik N. Photodynamic therapy as an alternative antimicrobial modality for oral infections. Journal of Environmental Pathology, Toxicology and Oncology. Iss 1-2, pp 546, Vol. 25, 2006.
[3]	Konopka K, Goslinski T. Photodynamic therapy in dentistry. J Dent Res; 86(8): 694-07, 2007.
[4]	Konopka K, Goslinski T. Prospects for photodynamic therapy in dentistry.http://www.photonics.com/Content/ReadArticle.aspx?ArticleID=34401.
[5]	Mantareva, V., V. Kussovski, I. Angelov, D. Wöhrle, R. Dimitrov, E. Popova, S. Dimitrov. Non - aggregated Ga(ΙΙΙ)-phthalocyanines in the photodynamic inactivation of planktonic and biofilm cultures of pathogenic microorganisms. Photochem. Photobiol. Sci., 10, 91-102, 2011.
[6]	Abdel-Shafi A, D Worrall. Mechanism of the excited singlet and triplet states quenching by molecular oxygen in acetonitrile. Journal of Photochemistry and Photobiology A: Chemistry. 172, 170-179, 2005.
[7]	Fernandez JM, MD Bilgin, LI Grossweiner. Singlet oxygen generation by photodynamic agents. Journal of Photochemistry and Photobiology B: Biology, 37, 131-140, 1997.
[8]	Mitra S, T Foster. Photochemical oxygen consumption sensitized by a Porphyrin phosphorescent probe in two model systems. Biophysical Journal, 78, 2597-2605, May 2000.
[9]	Wainwright M. Photodynamic antimicrobial chemotherapy (PACT). Journal of Antimicrobial Chemotherapy, 42, 13-28, 1998.
[10]	Josefsen L, Boyle R. Photodynamic therapy and the development of metal-based photosensitisers. Review article. Metal-Based Drugs, Vol. 2008; Article ID 276109, 24 pages.
[11]	Canavesi C, F Fournier, W Cassarly, T Foster, J Rolland. Illumination devices for photodynamic therapy of the oral cavity. Biomed Opt Express. 1(5): 1480-1490, 2010.
[12]	Кönig K, M Teschke, B Sigusch, E Glockmann, S Eick, W Pfister. Red light kills bacteria via photodynamic action. Cell Mol Biol (Noisy-le-grand). 46 (7): 1297-303, Nov 2000.
[13]	Bouillaguet S, Owen B, Wataha J, Campo M, Lange N, Schrenzel J. Intracellular reactive oxygen species in monocytes generated by photosensitive chromophores activated with blue light. Dent Mater, 24, 1070-1076, 2008.
[14]	Vlahova A. Possibilities of application of photodynamic therapy in prosthetic dental medicine. PhD dissertation, Plovdiv, Bulgaria 2012.
[15]	Vlahova. A., Chr. Kissov, E. Popova, I. Haydushka, V. Mantareva. A new method for photodynamic disinfection of prosthetic constructions and impressions in prosthetic dentistry. Folia Medica, Plovdiv, Bulgaria, 54(1), 51-57, 2012.
[16]	Vlahova A., Chr. Kissov, E. Popova. Photodynamic disinfection of dental impressions as a new competitive method to the conventional cleansing procedures. Journal of Analytical Oncology, 1(2), 187-191, Dec 2012.
[17]	Overman P. Biofilm: a new view of plaque. The Journal of Contemporary Dental Practice, 1(3), Summer Issue, 1-8, 2008.
[18]	Avon SL, JP Goulet, N Deslauriers. Removable acrylic resin disk as a sampling system for the study of denture biofilms in vivo. J Prosthet Dent; 97: 32-8, 2007.
[19]	Emami, E., J. Seguin, P. Rompre, L. Koninck, P. de Grandmont, J. Barbeau. The relationship of myceliated colonies of Candida albicans with denture stomatitis: An in vivo/in vitro study. Int J Prosthodont; 20: 514-520, 2007.
[20]	Zanin ICJ, MM Lobo, LKA Rodrigues, LAF Pimenta, JF Hofling, RB Goncalves. Photosensitization of in vitro biofilms by toluidine blue O combined with a light-emitting diode. Eur J Oral Sci; 114: 64-69, 2006
[21]	Bliss JM, CE Bigelow, TH Foster, CG. Haidaris. Susceptibility of Candida species to photodynamic effects of Photofrin. Antimicrobial Agents and Chemotherapy, 48(6): 2000-2006, June 2004.
[22]	Mima, E., A. Pavarina, L. Dovigo, C. Vergani, C. Costa, C. Kurachi, V. Bagnato. Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod; 109: 392-401, 2010.
[23]	Moura J., W. da Silva, T. Pereira, A. Cury, R. Garcia. Influence of acrylic resin polymerization methods and saliva on the adherence of four Candida species. J Prosthet Dent; 96: 205-11, 2006.
[24]	Serrano-Granger C., R. Cerero-Lapiedra, J. Campo-Trapero, J. Del Rio-Highsmith. In vitro study of the adherence of Candida albicans to acrylic resins: Relationship to surface energy. Int J Prosthodont; 18: 392-398, 2005.
[25]	Pereira T., A. Cury, M. Cenci, R. Rodrigues-Garcia. In vitro Candida colonization on acrylic resins and denture liners: influence of surface free energy, roughness, saliva, and adhering bacteria. Int J Prosthodont; 20: 308-310, 2007.
[26]	Darwazeh A, S Al-Refai, S Al-Mojaiwel. Isolation of Candida species from the oral cavity and fingertips of complete denture wearers. J Prosthet Dent; 86: 420-3, 2001.
[27]	ADA Council on Scientific Affairs and ADA Council on Dental Practice. Infection control recommendations for the dental office and the dental laboratory. JADA, 127: 672-680, May 1996.
[28]	Popova Е., Sl. Dimitrov, Chr. Kissov, A. Vlahova, I. Angelov, V. Mantareva, V. Kussovski. Photodynamic destruction of Candida albicans on dentures with porphyrin and phthalocyanines. 14-th Congress of BaSS. 9-th Scientific Congress of BgDA. Varna, Bulgaria, 6-9 May 2009.
[29]	http://en.wikipedia.org/wiki/Streptococcus_viridans.
[30]	http://en.wikipedia.org/wiki/Staphylococcus.
[31]	http://en.wikipedia.org/wiki/Neisseria.
[32]	http://en.wikipedia.org/wiki/Pneumococcus.
[33]	http://en.wikipedia.org/wiki/Corynebacterium.