Improved PCR-based Methods for Detecting Helical Forms of Helicobacter Pylori

Abdulaziz Radhi S ALjohni^1, , Bassam A Aljohny² and Sultan Saud Alahmadi³

¹Department of Laboratory at King Fahad Hospital, Medina P.O Box Building 4573, Zipcode 42392, Additional No 8925. Saudi Arabia

²Department of Biological Sciences, Faculty of Science, P.O. Box 80203, King Abdulaziz University, Jeddah, 21589, Saudi Arabia

³Head of Public Health Department at Ohud Hospital Family Medicine Registrar. Medina, Saudi Arabia

Cite this paper:
Abdulaziz Radhi S ALjohni, Bassam A Aljohny and Sultan Saud Alahmadi. Improved PCR-based Methods for Detecting Helical Forms of Helicobacter Pylori. American Journal of Infectious Diseases and Microbiology. 2022; 10(1):48-53. doi: 10.12691/ajidm-10-1-6

Abstract

The paper would address Helicobacter pylori which is a starting of bacteria in the gastrointestinal tract's human epithelial cells. His disorder is linked with many common illnesses, including recurrent gastritis, peptic ulcers, gastrointestinal lymphoma, and adenocarcinoma. More than 50 percent of the world is found to be infected with Helicobacter pylori. It is found deep inside the stomach of humans, where neutral HP predominates, in epithelial cells that are secreting mucus. This bacteria has been identified as the primary cause of a variety of lymphoma-related gastrointestinal illnesses, including chronic gastritis, excretory ulcer, abdominal cancer, and lymphoma of the gastric mucosa, among others (MALT). In this study, two PCR-based techniques for the detection of Helical types of Helicobacter pylori were improved. Molecular treatment of a strain of Helicobacter Pylori using amplification and direct analysis of 16S rDNA PCR.

Keywords:
molecular marker PCR gene diagnosis helicobacter pylori King Fahad Hospital

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