Some Nanoparticles Effects on Proteus sp. and Klebsiella Sp. Isolated from Water

Sahar M. Ouda^{1, 2,}

¹Department of Plant Research, Nuclear research Center, Atomic Energy Authority, Cairo, Egypt

²Biotechnology Department, Faculty of Science and Education (Girls), Al-Khurmah, Taif University, KSA

Cite this paper:
Sahar M. Ouda. Some Nanoparticles Effects on Proteus sp. and Klebsiella Sp. Isolated from Water. American Journal of Infectious Diseases and Microbiology. 2014; 2(1):4-10. doi: 10.12691/ajidm-2-1-2

Abstract

The purpose of the present study was to elucidate the antimicrobial activity and mechanism of silver, platinum, palladium and copper nanoparticles on Proteus sp. and Klebsiella Sp. isolated from polluted water samples. This study investigated the growth of the two tested bacterial cells in order to observe the action of different concentrations of nanoparticles in addition to the effects on the morphological structure and nucleic acid of these bacteria, It showed AgNPs exhibited a potentially antibacterial effects at most investigated concentrations, While, no detection for the antibacterial activity of PtNPs, PdNPs or CuNPs against both bacterial isolates at all tested concentrations was investigated. The cell morphology of normal and AgNPs-treated bacteria cells was assessed by transmission electron microscopy (TEM), there were investigated a randomly distributed nuclear area and obvious bright electron areas in the center of bacterial cells, it was also noticed that, a rupture in the cell wall of both treated bacterial cells. The effects of silver nanoparticles on genome of bacterial cells revealed that there was a destructive effect on Proteus and Klebsiella genome.

Keywords:
metal nanoparticles metal nanoparticles antibacterial activity action mechanism Proteus sp. Klebsiella sp.

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References:

[1]	Morose, G., “The 5 principles of Design for Safer Nanotechnology,” Journal of Cleaner Production, 3. 285-289.2010
[2]	Dhermendra K. Tiwari, J. Behari and Prasenjit Sen., “Application of Nanoparticles in Waste Water Treatment,” World Applied Sciences Journal 3(1). 417-433. 2008.
[3]	Michael A. Perez., “The effects of silver nanoparticles on wastewater treatment and Esherichia coli growt,” A Thesis submitted to the Department of Civil and Environmental Engineeringin partial fulfillment of the requirements for graduation with Honors in the Major. The Florida State University. 2012.
[4]	Amany A. El-Kheshen and Sanaa F. Gad El-Rab., “Effect of reducing and protecting agents on size of silver nanoparticles and their anti-bacterial activity,” Der Pharma Chemica. 4. 53-65.2012.
[5]	Gutierrez FM, Olive PL, Banuelos A, Orrantia E, Nino N, Sanchez EM, Ruiz F, Bach H, Gay YA., “Synthesis, characterization, and evaluation of antimicrobial and cytotoxic effect of silver and titanium nanoparticles,” Nanomedicine, 6. 681-688. 2010
[6]	Abbott, S. L., “Klebsiella, Enterobacter, Citrobacter, Serratia, Plesiomonas, and Other Enterobacteriaceae,” In P. R. Murray, E. J. Baron, J. H. Jorgensen, M. L. Landry & M. A. Pfaller (Eds.), Manual of Clinical Microbiology, 9th ed. 698-711. 2007 Washington, USA: ASM Press.
[7]	Euzéby, J. P., “List of Bacterial Names with Standing in Nomenclature,” International Journal of Systmatic Bacteriology. 47. 13. 2010.
[8]	Janda, J. M., & Abbott, S. L., “The Genera Klebsiella and Raoultella,” The Enterobacteria, 2nd ed. 115-129. 2006 Washington, USA: ASM Press.
[9]	Seham Abdel-Shafi, Sahar M. Ouda, Ibrahim Elbalat and Gamal Enan., “Characterization and Identification of Multidrug Resistant Bacteria from Some Egyptian Patients,” Biotechnology. 12. 65-73.2013.
[10]	Li WR, Xie XB, Shi QS, Zeng HY, Ou-Yang YS, Chen YB., “Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli,” Applied Microbiology and Biotechnology, 85(4). 1115-1122. 2010.
[11]	Meiwan Chen, Zhiwen Yang, Hongmei Wu, Xin Pan, Xiaobao Xie and Chuanbin Wu, “Antimicrobial activity and the mechanism of silver nanoparticle thermosensitive gel,” Intertional Journal Nanomedicine, 6: 2873-2877. 2011.
[12]	Muthu Irulappan Sriram Selvaraj Barath Mani Kanth Kalimuthu Kalishwaralal Sangiliyandi Gurunathan., “Antitumor activity of silver nanoparticles in Dalton’s lymphoma ascites tumor model,” International Journal of Nanomedicine. 5.753-762. 2010.
[13]	Parameswari E., Udayasoorian C., Paul Sebastian S. and Jayabalakrishnan R. M., “The bactericidal potential of Ag nanoparticles,” International Research Journal of Biotechnology, 1.3. 044-049. 2010.
[14]	Petrus, E.M., Tinakumari, S., Chai, L. C., Ubong, A., Tunung, R., Elexson, N., Chai, L. F. and Son, R., “A study on the minimum inhibitory concentration and minimum bactericidal concentration of Nano Colloidal Ag on food-borne pathogens,” International Food Research Journal, 18. 55-66. 2011.
[15]	Sai MA, Naomi IZUTA, Satoshi IMAZATO, Ji-hua CHEN, Wakako KIBA, Ranna YOSHIKAWA, Kahoru TAKEDA, Haruaki KITAGAWA and Shigeyuki EBISU., “Assessment of bactericidal effects of quaternary ammonium-based antibacterial monomers in combination with colloidal platinum nanoparticles,” Dental Materials Journal, 31(1). 150-156. 2012.
[16]	Cho K, Park J, Osaka T, Park S., “The study of antimicrobial activity and preservative effects of nanosilver ingredient,” Electrochim Acta, 51. 956-60. 2005.
[17]	Lok C et al., “Proteomic analysis of the mode of antibacterial action of silver nanoparticles,” J Proteome Res. 2006; 5:916-24.
[18]	Yamanaka M, Hara K, Kudo J., “Bactericidal actions of a silver ion solution on Escherichia coli, studied by energy filtering transmission electron microscopy and proteomic analysis,” Applied Environmental Microbiology, 71. 7589. 93.2005.
[19]	Singh,M., Singh, S. Prasada, S. and Gambhir, I. S., “Nanotechnology In Medicine and Antibacterial Effect Of Ag Nanoparticles,” Digest Journal Of Nanomaterials And Biostructures, 3. 115-122. 2008.
[20]	Ravishankar Rai V and Jamuna Bai A., “Nanoparticles and their potential application as antimicrobials. Science against microbial pathogens,” communicating current research and technological advances. A. Méndez-Vilas (Ed.), 197-209. 2011
[21]	Shrivastava S, Bera T, Roy A, Singh G, Ramachandrarao P, Dash D., “Characterization of enhanced antibacterial effects of novel silver nanoparticles,”. Nanotechnology. 2007; 18:1-9.
[22]	Bard AJ, Holt KB., “Interaction of Silver(I) Ions with the Respiratory Chain of Escherichia coli: An Electrochemical and Scanning Electrochemical Microscopy Study of the Antimicrobial Mechanism of Micromolar Ag+,”.Biochemistry, 44. 13214-13223. 2005.
[23]	Dibrov P, Dzioba J, Gosink KK, Hase CC., “Chemiosmotic mechanism of antimicrobial activity of Ag+ in Vibrio cholerae,”. Antimicrob. Agents Chemother. 2002; 46: 2668-2670. 2002.