Impact of Peroxisome Proliferator-Activated Receptor Gamma SNP C161T gene on Lipid Parameters Linked to Diabetes Mellitus in Cotonou (Benin)

Nicodème W. Chabi^{1, 2,} , Martin N. CAKPO AKLAN³, Adé M. Dominique SANNI², Christian T. R. KONFO⁴, Jonas SOSSA-HIFFO², Daniel K. AMOUSSOU-GUENOU⁵, DAHOUENON-AHOUSSI Edwige⁴ and BABA-MOUSSA Lamine⁶

¹Laboratory of Teaching and Research in Food Microbiology, Department of Food Technology Engineering, Polytechnic School of Abomey, University of Abomey, 01P.BP 2009 Cotonou, Benin

²Laboratory of Biochemistry and Molecular Biology, Department of Biochemistry and Cell Biology, Faculty of Science and Technology (FAST), University of Abmomey-Calavi, Cotonou, Benin

³Pharmacy Training and Research Unit, Faculty of Health Sciences, University of Abmomey-Calavi, Cotonou, Benin

⁴Laboratory of Study and Research in Applied Chemistry, Polytechnic School of Abomey, University of Abomey-calavi, 01 BP 2009 Cotonou, Benin

⁵Department of Endocrinology-Metabolism, National Hospitals University Center, Faculty of Health Sciences, University of Abmomey-Calavi, Cotonou, Benin

⁶Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology (FAST), University of Abmomey-Calavi, 05 BP 1604, Cotonou, Benin

Cite this paper:
Nicodème W. Chabi, Martin N. CAKPO AKLAN, Adé M. Dominique SANNI, Christian T. R. KONFO, Jonas SOSSA-HIFFO, Daniel K. AMOUSSOU-GUENOU, DAHOUENON-AHOUSSI Edwige and BABA-MOUSSA Lamine. Impact of Peroxisome Proliferator-Activated Receptor Gamma SNP C161T gene on Lipid Parameters Linked to Diabetes Mellitus in Cotonou (Benin). American Journal of Biomedical Research. 2017; 5(3):39-45. doi: 10.12691/ajbr-5-3-1

Abstract

Diabetes is one of the most prevalent metabolic diseases worldwide. Prevent or delay the onset of diabetes may be the better management of this condition. It is in this context that we have chosen to investigate the genes involved in the homeostasis of blood sugar and lipids. The purpose of this study was to examine the effects of the C161T polymorphism Peroxisome proliferator-activated receptor gamma (PPARɣ) on lipid parameters in Benin subjects (n=41) diagnosed with type 2 diabetes and controls (n=8). Anthropometric data in consideration were: body mass index (BMI), height, weight, waist circumference, hip turn. The biochemical parameters in consideration were: fasting glucose, total cholesterol (TC), HDL-cholesterol and LDL-cholesterol, triglycerides (TG) and total protein.Genomic DNA was obtained from white blood cells present in the buffy coats of type 2 diabetic subjects and controls. The mutations analysis in the coding region of PPAR gene was carried out by PCR assay while the C161T polymorphism was analysed by PCR-RFLP. It is clear that obesity was a prognostic factor for type 2 diabetes (p =0.05). Using the correlation test, on one hand we observed a correlation between BMI, sex, height and weight, respectively, p= 0.051; p <0.001; p<0.001; and secondly a correlation between BMI and lipid parameters, p =0.012. The frequency of the wild allele '' C '' of the C161 polymorphism was 63.27% and that of the allele transferred '' T'' (T161) was 36.73%. No significant difference was observed between the SNP C161T and lipid variables TC, TG, HDL-C, LDL-C (p> 0.05). However, we observed a low level of TG in carriers '' TT '' than for '' CT '' and "CT +TT" (0.17g / L TT vs 0.73g / L CT vs0.69g / CT + TT). The results show that the C161T polymorphism in the PPARɣ gene is not associated with blood lipid variables in type 2 diabetes of the study population. However, a reduction of triglycerides in serum was observed in individuals carrying the variant '' TT '' C161T polymorphism of the PPARɣ gene.

Keywords:
PPARɣ PCR-RFLP SNP C161T diabetes mellitus blood lipid parameters BMI

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References:

[1]	Organisation Mondiale de la Santé. 10 faits sur les maladies non transmissibles. [En ligne]. 2013 [Consulté le 24 Août 2015] URL<www.who.int/features/factifiles/noncommunicable_deseases/fr/>
[2]	Jenkins, A. J., Hill, M. A., & Rowley, K. G. (2008). Diabetes and oxidant stress. In Atherosclerosis and Oxidant Stress (pp. 123-158). Springer US. A New Perspective. Holtzman J.L 2007 (ed).p123-160.
[3]	Prabhakar, P. K., & Doble, M. (2011). Mechanism of action of natural products used in the treatment of diabetes mellitus. Chinese Journal of Integrative Medicine, 17(8), 563-574.
[4]	Hall, V., Thomsen, R. W., Henriksen, O., & Lohse, N. (2011). Diabetes in Sub Saharan Africa 1999-2011: epidemiology and public health implications. A systematic review. BMC public health, 11(1), 564.
[5]	Djrolo, F., Amoussou-Guenou, K. D., Zannou, D. M., Houinato, D., Ahouandogbo, F., & Houngbe, F. (2003). Prévalence du diabète sucré au Bénin. Louvain médical, 122(6), S256-S260.
[6]	Programme National de Lutte contre les maladies Non Transmissibles. (2008). Rapport final de l’enquête STEPS Bénin: Programme de Lutte contre les Maladies Non Transmissibles;.
[7]	Djrolo, F. (2009). P105 Prévalence du diabète sucré dans une population urbaine en milieu africain à Cotonou–Bénin. Diabetes & Metabolism, 35, A53.
[8]	Stumvoll, M., Goldstein, B. J., & van Haeften, T. W. (2005). Type 2 diabetes: principles of pathogenesis and therapy. The Lancet, 365(9467), 1333-1346.
[9]	Rangwala, S. M., & Lazar, M. A. (2004). Peroxisome proliferator-activated receptor γ in diabetes and metabolism. Trends in pharmacological sciences, 25(6), 331-336.
[10]	Fossati, P. and L. Principe, Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clinical chemistry, 28(10): 2077-2080. 1982.
[11]	Doumas, B. T., Bayse, D. D., Carter, R. J., Peters, T., & Schaffer, R. (1981). A candidate reference method for determination of total protein in serum. I. Development and validation. Clinical Chemistry, 27(10), 1642-1650.
[12]	Savage, D. B., Choi, C. S., Samuel, V. T., Liu, Z. X., Zhang, D., Wang, A., ... & Bhanot, S. (2006). Reversal of diet-induced hepatic steatosis and hepatic insulin resistance by antisense oligonucleotide inhibitors of acetyl-CoA carboxylases 1 and 2. The Journal of clinical investigation, 116(3), 817-824.
[13]	Wan, J., Xiong, S., Chao, S., Xiao, J., Ma, Y., Wang, J., & Roy, S. (2010). PPARγ gene C161T substitution alters lipid profile in Chinese patients with coronary artery disease and type 2 diabetes mellitus. Cardiovascular diabetology, 9(1), 13.
[14]	Ting-Hsing, C. H. A. O., Yi-Heng, L. I., Jyh-Hong, C. H. E. N., Hua-Lin, W. U., Guey-Yueh, S. H. I., Ping-Yen, L. I. U., ... & How-Ran, G. U. O. (2004). The C161T genotype in the exon 6 of the peroxisome-proliferator-activated receptor γ gene is associated with premature acute myocardial infarction and increased lipid peroxidation in habitual heavy smokers. Clinical Science, 107(5), 461-466.
[15]	Adanchede SA. Etude du suivi diabétologique et de la pathologie cardiovasculaire dans une population de 102 patients diabétiques constituée en 2003. Thèse médecine: Cotonou; 2011.
[16]	Azebaze AP. (2004). Les artériopathies diabétiques des membres inférieurs dans le service de médecine interne de l’hôpital du point-g (Doctoral dissertation, Thèse de médecine, Bamako).
[17]	Gning, S. B., Thiam, M., Fall, F., Ba-Fall, K., Mbaye, P. S., & Fourcade, L. (2007). Le diabete sucre en Afrique subsaharienne. Aspects epidemiologiques, difficultes de prise en charge. Médecine tropicale, 67(6), 607-611.
[18]	Houénassi M, Amoussou-Guénou KD, Abattan S, et al. Epidémiologie de l’artériopathie chronique oblitérante des membres inférieurs chez le diabétique au CNHU de Cotonou (Bénin). Arch. Mal. Cœur 2004; 97 (12): 1189-94.
[19]	Tai ES, Dolores C, Mabel DY, Xian A, Suok KC, Chee ET et al.Differential effects of the C1431T and Pro12Ala PPAR gene variants on plasma lipids and diabetes risk in an Asian population.J. Lipid Res2004; 45: 674-85.
[20]	Tavares, V., Hirata, R. D., Rodrigues, A. C., Monte, O., Salles, J. E., Scallissi, N., ... & Hirata, M. H. (2005). Effect of the peroxisome proliferator-activated receptor-gamma C161T polymorphism on lipid profile in Brazilian patients with Type 2 diabetes mellitus. J Endocrinol Invest, 28(2), 129-136.
[21]	Li, Q., Chen, R., Bie, L., Zhao, D., Huang, C., & Hong, J. (2015). Association of the variants in the PPARG gene and serum lipid levels: a meta‐analysis of 74 studies. Journal of cellular and molecular medicine, 19(1), 198-209.
[22]	Mattevi, V. S., Zembrzuski, V. M., & Hutz, M. H. (2007). Effects of a PPARG gene variant on obesity characteristics in Brazil. Brazilian Journal of Medical and Biological Research, 40(7), 927-932.
[23]	Bhatt, S. P., Nigam, P., Misra, A., Guleria, R., Luthra, K., Pandey, R. M., & Pasha, M. Q. (2013). Association of peroxisome proliferator activated receptor-γ gene with non-alcoholic fatty liver disease in Asian Indians residing in north India. Gene, 512(1), 143-147.