American Journal of Biomedical Research
ISSN (Print): 2328-3947 ISSN (Online): 2328-3955 Website: Editor-in-chief: Hari K. Koul
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American Journal of Biomedical Research. 2014, 2(4), 83-87
DOI: 10.12691/ajbr-2-4-5
Open AccessArticle

Impacts of Herbicide Exposure on Seminal Parameters among Oil Palm Plantation Workers in Lampung Province, Indonesia

Sutyarso1 and M. Kanedi2,

1Department of Biomedical Sciences, Faculty of Medicine, University of Lampung, Indonesia

2Department of Biology, Faculty of Mathematics and Sciences, University of Lampung, Indonesia

Pub. Date: December 30, 2014

Cite this paper:
Sutyarso and M. Kanedi. Impacts of Herbicide Exposure on Seminal Parameters among Oil Palm Plantation Workers in Lampung Province, Indonesia. American Journal of Biomedical Research. 2014; 2(4):83-87. doi: 10.12691/ajbr-2-4-5


It is a cross-sectional study to evaluate semen quality of plantation workers that due their daily obligation make them susceptible to herbicide exposures. The research participants were male workers of oil palm plantations in the District of Lampung Tengah, Lampung Province, Indonesia. They were those who meet the criteria of having more than one year work experience with herbicide, married and having children. Semen samples were taken by asking the participants to masturbate after they were advised to abstain from sexual intercourse for 4-5 days. Semen quality parameters that examined are volume, pH, sperm count, sperm motility, normal morphology and HOS-test. Based on years of the work periods, there were three groups of participants obtained. Group-1 are the participants who worked less than 10 years; group-2 are those who have work periods of 10-20 years; while group-3 are those who have worked more than 20 years. All of the semen quality parameters, except pH, decreased significantly with the increase in work periods far beyond the maximum decrease in normal elderly male. In conclusion, the daily tasks of oil palm plantation workers which prompt them expose to herbicide, suspected to be the cause of the decrease in semen quality parameters.

pesticide herbicide semen parameters plantation workers

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[1]  USDA, Indonesia: Rising Global Demand Fuels Palm Oil Expansion. United States Department of Agriculture. 2010.
[2]  UNEP, Oil palm plantations: threats and opportunities for tropical ecosystems. UNEP Global Environmental Alert Service (GEAS). 2011.
[3]  Mohamad RB, Wibawa W, Mohayidin MG, Puteh AB, Juraimi AS, Awang Y and Lasim MBM. “Management of Mixed Weeds in Young Oil-palm Plantation with Selected Broad-Spectrum Herbicides,” Pertanika J. Trop. Agric. Sci. 2010; 33 (2): 193-03.
[4]  WHO, The WHO recommended classification of pesticides by hazard and guidelines to classification. 2005.
[5]  Karr, C.J., Solomon, G.M. and Brock-Utne, A.C. “Health Effects of Common Home, Lawn, and Garden Pesticides,” Pediatr Clin N Am 2007; 54: 63-80.
[6]  Séralini GE, Clair E, Mesnage R, Gress S, Defarge N, Malatesta M, Hennequin D and de Vendômoiset JS. Long term toxicity of a Roundup herbicide and a Roundup-tolerant genetically modified maize. Food Chem Toxicol 2012.
[7]  Tuc VP, Wangsuphachart V, Tasanapradit P, Fungladda W, van Trong P and Nhung NT. “Impacts of pesticide use on semen characteristics among rice farmers in Kienxuong district, Thaibinh Province, Vietnam,” Southeast Asian J Trop Med Public Health 2007; 38:569-75.
[8]  Hossain F, Ali O, D’Souza UJA and Naing DKS. “Effects of pestcide use on semen quality among farmers in rural area of Sabah, Malaysia,” Journal of Occupational Health 2010; 52: 353-60.
[9]  Sekiyama M, Tanaka M, Gunawan B, Abdoellah O and Watanabe C, “Pesticide Usage and Its Association with Health Symptoms among Farmers in Rural Villages in West Java, Indonesia,” Environmental Sciences 2007; 14(Supplement 2007): 023-33.
[10]  WHO, The WHO Laboratory Manual for The Examination and Processing of human semen. 5th ed. WHO Press. Geneva, Switzeland. 2010.
[11]  Hossain, A.M., Rizk, B., Barik, S., Huff, C. and Thorneycroft, I.H., Time course of hypo-osmotic swellings of human spermatozoa: evidence of ordered transition between swelling subtypes.” Human Reproduction 1998; 13(6): 1578-1583.
[12]  WHO Expert Consultation, “Appropriate body-mass index for Asian populations and its implications for policy and intervention strategies,” Lancet 2004; 363: 157-63.
[13]  MacDonald AA, Stewart AW and Farquhar CM. “Body mass index in relation to semen quality and reproductive hormones in New Zealand men: a cross-sectional study in fertility clinics,” Human Reproduction 2013; 0(0): 1-10.
[14]  Molina RI, Martini AC, Tissera A, Olmedo J, Senestrari D, de Cuneo MF and Ruiz RD., “Semen Quality and Aging: Analysis of 9.168 Samples in Cordoba, Argentina,” Arch. Esp. Urol 2010; 63 (3): 214-221.
[15]  Kidd SA, Eskenazi B and Wyrobek AJ. “Effects of male age on semen quality and fertility: a review of the literature,” Fertility and Sterility 2001; 75(2):237-48.
[16]  Akan JC, Mohammed Z, Jafiya L and Audu SI., “Organophosphorus Pesticide Residues in Different Tissues of Fish Samples from Alau Dam, Borno State, Nigeria,” World Journal of Fish and Marine Sciences 2013; 5(5): 519-26.
[17]  Smalling KL, Fellers GM, Kleeman PM and Kuivila KM. “Accumulation of Pesticides in Pacific Chorus Frogs (Pseudacris regilla) from California’s Sierra Nevada Mountains, USA,” Environmental Toxicology and Chemistry 2013; 32(9): 2026-34.
[18]  Deshmukh US, Iram F and Joshi MS. “Effect of Acute Exposure of Glyphosate Herbicide, on Wistar Rats with Reference to Haematology and Biochemical Analysis,” The Bioscan 2013; 8(2): 381-3.
[19]  Ye M, Beach J, Martin JW and Senthilselvan A. Occupational Pesticide Exposures and Respiratory Health,” Int. J. Environ. Res. Public Health 2013; 10: 6442-71.
[20]  Jurewicz, J, Hanke W, Radwan M and Bonde JP. “Environmental factors and semen quality,” Int J Occup Env Health 2010; 22: 305-29.
[21]  Hossain, F., Ali, O., D’Souza, U.J.A. and Naing, D.K.S., “Effects of pestcide use on semen quality among farmers in rural area of Sabah, Malaysia.,” Journal of Occupational Health, 2010, 52: 353-360.
[22]  D’Souza UJA, Narayana K, Zain A, Raju S, Nizam HM and Noriah O. “Dermal exposure to the herbicide paraquat results in genotoxic and cytotoxic damage to germ cells in the male rat,” Folia Morphol 2006; 65(1): 6-10.
[23]  Gasnier, C., Dumont, C., Benachour, N., Clair, E., Chagnon, M.C. and Seralini, G.E., “Glyphosate-based herbicides are toxic and endocrine disruptors in human cell lines,” Toxicology 262 (2009): 184-191.
[24]  Joshi SC, Tibrewal P, Sharma A and Sharma P. “Evaluation of toxic effect of 2,4-D (2,4-dichlorophenoxy acetic acid) on fertility and biohemical parameters of male reproductive system of albino rats,” Int J Pharm Pharm Sci 2012; 4(3): 338-42.