Wound Healing Activities of Latex of Grassleaf Spurge (Euphobia graminea Jacq.)

Morakinyo A. E.^1, , Akinpelu B. A.², Bolaji A. O.³, Adelowo J. M.⁴, Babarinde S. O.¹, Orimolade O.O¹, Oladimeji O. D.¹, Adewola O. O.¹ and Oyedapo O. O²

¹Department of Biochemistry, Adeleke University, Ede, Osun State

²Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile-Ife, Osun State

³Department of Botany, Obafemi Awolowo University, Ile-Ife, Osun State

⁴Department of Basic Sciences, Adeleke University, Ede, Osun State

Cite this paper:
Morakinyo A. E., Akinpelu B. A., Bolaji A. O., Adelowo J. M., Babarinde S. O., Orimolade O.O, Oladimeji O. D., Adewola O. O. and Oyedapo O. O. Wound Healing Activities of Latex of Grassleaf Spurge (Euphobia graminea Jacq.). American Journal of Biomedical Research. 2020; 8(3):63-71. doi: 10.12691/ajbr-8-3-2

Abstract

The present study evaluated the wound healing potentials of E. graminea (EG) latex. The latex of EG was screened for the presence of phytochemical constituents; in vitro antioxidant property and erythrocyte membrane stabilizing activity was used to investigate anti-inflammatory property of the latex. The wound healing potential of E. graminea latex (EGL) was investigated via incision and dead space wound models in wistar rats; eighteen wistar rats (separately for each model) were randomly divided into 3 groups (6 in each group): Group A: wound + EGL ; Group B: wound + Gentamycin (G); Group C: wound without treatment (control). Wound breaking strength (Incision model), hexuronic acid and hexosamine concentrations, activities of antioxidants biomolecules (superoxide dismutase, catalase, and reduced glutathione) as well as the levels of free radicals (nitric oxide-NO, malondialdehyde -MDA) were evaluated in the granulation tissue (dead space model) of experimental rats. The result of the study revealed that EGL tested positive for the presence of saponins, alkaloids, triterpenes, flavonoids and cardiac glycosides. Also, EGL contained an appreciable concentration of total flavonoids and phenolics. The latex exhibited mild ferric reducing power, inhibited DPPH in dose dependent manner as well as protected red blood cells against hypotonic and heat induced lyses. In wound incision model, EGL exhibited 13.6 % increase in wound breaking strength when compared to the control animals. Also, in dead space wound model, there was significant increase (p < 0.05) in hexoxamine, hexuronic acid and GSH concentrations as well as SOD activity in EGL and gentamycin treated wound compared to the control. Furthermore, there was a significant reduction (p < 0.05) in MDA concentration in EGL and gentamycin treated wound compared to the control while NO level and catalase activity showed no significant difference. This study revealed that EGL contained potentially bioactive molecules that could be employed in the treatment of wound.

Keywords:
euphorbia graminea latex wound healing

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References:

[1]	Nagor, B.P., Solanki, R., “Role of medicinal plants in wound healing,” Journal of Medicinal Plant Research 5: 392-405. 2011.
[2]	Das, J., Injury and Wound Healing, In: J. Das (ed.) Trauma in otolaryngology Springer Singapore, Singapore, 2018, 31-52.
[3]	Mitzzy, F., Medellín-Luna, J., YordaniMartínez-Balderas, V., Cervantes-Villagrana, A.R., “Medicinal plant extracts and their use as wound closure inducing agents,” Journal of Medicinal Food, 22: 435-443. 2019.
[4]	Kumar, B., Vijayakumar, M., Govindarajan, R., Pushpangadan, P., “Ethnopharmacological approaches to wound healing-exploring medicinal plants of India,” Journal of Ethnopharmacology, 114: 103-113. 2007.
[5]	Oguntibeju, O., “Medicinal plants and their effects on diabetic wound healing,” Veterinary World, 12 (5): 653-663. 2019.
[6]	McVaugh, R. Euphorbiae Novo-Galicianaerevisae. Contributions to University of Michigan Herbarium, 1993, 207-239.
[7]	Betancur-Galvis, L.A., Morales, G.E., Forero, J.E., Roldan, J., “Cytotoxic and antiviral activities of Colombian medicinal plant extracts of the Euphorbia genus,” Mem Inst Oswaldo Cruz, 97 (4): 541-546. 2002.
[8]	Trease, G.E., Evans, W.C., Pharmacogosy.15th Edn. Saunders 2002, 214-393..
[9]	Sofowora, E.A. Medicinal plants and traditional medicine in Africa, Second Edition, Spectrum Books Limited, Ibadan, Nigeria, 2006, 150-153.
[10]	Singleton, V., Orthofer, R., Lamuela, R., “Analysis of total phenol and other oxidation substrates and antioxidant by means of folin- ciocalteu reagent,” Methods Enzyme, 299: 152-178. 1999.
[11]	Aina, O.I., Oyedapo, O.O., “In vitro investigations into the antioxidant and antiinflammatory potentials of the fractions and ethanolic extract of Cyclosorus afer (Christ.) Ching, stalks,” Ife Journal of Science, 15 (2): 235-249. 2013.
[12]	Sun, P.X., Yie, L.K., Zhang, Z.L., Hu, M., Lu, L, “Colometric determination of the total content of the flavonoids in epicedium capsules,” Journal of Shenyang Pharmaceutical University, 16: 68-70. 1999.
[13]	Oyaizu, M., “Studies on products of browning reaction. Antioxidative activities of browning reaction products prepared from glucosamine,” Japanese Journal Nutrition, 44: 307-315. 1986
[14]	Konate, K., “ In vitro antioxidant , lipoxiginase and xanthine-oxidase inhibitory activities of fractions from Cienfuegosia digitata Cav. Sida alba L. and Sida acuta Bum. F. (Mavaceae),” Pakistan Journal of Biological Science, 13 (22): 1092-1098. 2010.
[15]	Oyedapo, O. O., Akinpelu, B. A., Orfuwa, S. O., “Anti-inflammatory effects of Theobroma cacao, root extract,” Journal of Tropical Medicinal Plants, 5: 161-165. 2004.
[16]	Bode, S.O., Oyedapo, O.O., “Biological activities and phytoconstituents of the lower plant Platycerium anglolense, Welwex Hook,” Journal of Medicinal Plants Research, 5: 1321-1329. 2011.
[17]	NIS National Institutes of Health NIH Laboratory Animal Welfare Guide for Grants and Contracts, 14 (8): 25. 1985.
[18]	Agrawal, N., Patil, M., Chimkode, R., Tripathi, A., “Antimicrobial and Wound Healing Activities of Leaves of Alternanthera sessilis Linn.,” International Journal of Green Pharmacy, 1: 141-144. 2000.
[19]	Lee, K., “Studies on the mechanism of action of salicylates III, effect of vitamin A on the wound healing retardation action of aspirin,” Journal of Pharmaceutical Science, 57: 1238-1240. 1968.
[20]	Agarwal, P. K., Singh, A., Gaurav, K., Goel, S., Khanna, H. D. Goel, R. K., “Evaluation of wound healing activity of extracts of plantain banana (Musa sapientum var. paradisiaca) in rats,” Indian Journal of Experimental Biology, 47 (1): 32-40. 2009.
[21]	Murthy, S., Gautam, M. K., Goel, S., Purohit, V., Sharma, H. Goel, R. K. “Evaluation of in-vivo wound healing activity of bacopa monniera on different wound model in rats,” Biomed Research International 1-9. 2013.
[22]	Grisham, M.B., Johnson, M.D., Gautreaux, R.D., Methods, A Comp. to Meth. in Enz., 7: 84-90. 1995.
[23]	Ohkawa, H., Ohishi, N., Yagi, K., “Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction,” Analytical Biochemistry, 95 (2): 351-358. 1979.
[24]	Beutler, E., Duron, O., Kefly, B. M., “Improved method for the determination of blood glutathione,” Journal of Laboratpry and Clinical Medicine, 61: 882-888. 1963.
[25]	McCord, J.M., Fridovich, I., “Superoxide dismutase: An enzymic function for erythrocuprein (hemocuprein),” Journal of Biological Chemistry, 244(22): 6049-6055. 1969.
[26]	Aebi, H.E., Catalase. In: Bergmeyer, H. U. Methods of Enzymatic Analysis, Vol. 3 (3rd ed.), VerlagChemie, Weinheim, Germany, 273-286. 1982.
[27]	Dische, Z., Borenfreund, E., “A spectrophotometric method for the micro determination of hexosamines” Journal of Biological Chemistry, 184: 517-522. 1950.
[28]	Bitter, T., Muir H., “A modified uronic acid carbazole reaction,” Anaytical Biochemistry, 4 (4): 330-334. 1962.
[29]	Nayak, B. S., Pinto Pereira, M., “Catharanthus roseus flower extract has wound healing activity in Sprague Dawley rats,” BMC Complementary and Alternative Medicine, 2006.
[30]	Kumar, M.S., Kirubanandan, S., Sripriya, R., Sehgal, P. K., “Triphala promotes healing of infected full-thickness dermal wound”, Journal of Surgical Research, 144(1): 94-101. 2008.
[31]	Jonas, I., Anthony, S., Afolayan, J., Bradley, G., ”Antioxidant, antibacterial and phytochemical properties of two medicinal plants against the wound infecting bacteria,” Asian Pacific Journal of Tropical Biomedicine, 7(9): 817-825. 2017.
[32]	Aryani, R., AgungNugroho, R., Manurung, H., R. Mardayanti, Rudianto, W. Prahastika, Auliana, A. BruKaro, P., “Ficus deltoidea leaves methanol extract promote wound healing activity in mice,” Eurasia Journal of Bioscience, 14: 85-91. 2020.
[33]	Kamath, J.V., Rana, A.C., Chowdhury, A.R., “Pro-healing effect of Cinnamomum zeylanicum bark,” Phytotheraphy Research, 17: 970-972. 2003.
[34]	Saroja, M., Santhi, R., Annapoorani, S., “Wound healing activity of flavonoid fraction of Cynodon dactylon in swiss albino mice,” International Research Journal of Pharmacy, 3: 230-231. 2012.
[35]	Kumar, S., Pandey, A., “Chemistry and biological activities of flavonoids: an overview,” Science World Journal 2013.
[36]	Muralidhar, A., Babu, S., Sankar, R., Reddanna, P., Latha, J. “Wound healing activity of flavonoid fraction isolated from the stem bark of Buteamono sperma (Lam) in albino wistar rats,” European Journal of Experimental Biology, 3(6): 1-6. 2013.
[37]	Ghasemzadeh, A., Ghasemzadeh, N. “Flavonoids and phenolic acids: Role and biochemical activity in plants and human,” Journal of medicinal plant research., 5(31): 6697-6703. 2011.
[38]	Sandhar, H., Kumar, B., Prasher, B., Tiwari, P., Salhan, M., Sharma, P., “A review of phytochemistry and pharmacology of flavonoids,” Internationale Pharmaceutica Sciencia, 1 (1): 25-41. 2011.
[39]	Stoilova, I., Krastanov, A., Stoyanova, A., Denev, P., Gargova, S., “Antioxidant activity of a ginger extract Zingier officinale,” Food Chemistry, 102: 764-770. 2007.
[40]	Nsimba, R., Kikuzaki, H., Konishi, Y., “Antioxidant activity of various extract fractions of Chenopodium quinoa and Amaraathus sp. Seeds,” Food Chemistry, 106: 760-766. 2008.
[41]	Reddy, B. S., Reddy, R. K. K., Naidu,V. G. M., Madhusudhana, K., Agwane, S. B., Ramakrishna, S., “Evaluation of antimicrobial, antioxidant and woundhealing potentials of Holoptelea integrifolia,” Journal of Ethnopharmacology. 115:249 56. 2008.
[42]	Mensah, A. Y., Sampson, J., Houghton, P. J., Hylands, P. J., Westbrook, J., Dunn, C. M., “Effects of Buddleja globosa leaf and its constituents relevant to wound healing,” Journal of Ethnopharmacology, 77:219-26. 2001.
[43]	Jorge, M. P., Madjarof, C., Ruiz, A. L., Fernandes, A. T., Rodrigues, R. A. F., Sousa, I. M. O., Foglio, M. A., Carvalho, J. E., “Evaluation of wound healing properties of Arrabidaea chica Verlot extract,” Journal of Ethnopharmacology, 118: 361-6. 2008.
[44]	Houghton, P.J., Hylands, P. J., Mensah, A. Y., Hensel, A., Deters, A. M., “In vitro tests and ethnopharmacological investigations: wound healing as an example,” Journal of Ethnopharmacology, 100: 100-7. 2005.
[45]	Annan, K., Houghton, P. J., “Antibacterial, antioxidant and fibroblast growth stimulation of aqueous extracts of Ficus asperifolia Miq. and Gossypium arboreum L., wound-healing plants of Ghana,” Journal of Ethnopharmacology, 119: 141-4. 2008.
[46]	Süntar, I., Akkol, E., Nahar, L., Sarker, S. “Wound healing and antioxidant properties: do they coexist in plants?,” Free Radicals and Antioxidants, (1): 1-8. 2012.
[47]	Shirwaikar A. Shenoy R. Udupa A.L. Udupa S.L. Shetty S., “Wound healing activity of phenolic extract of leaves of Hyptis suaveolens with supportive role of antioxidant enzymes,” Indian Journal of Experimental Biology, 41: 238-241. 2003.
[48]	Kováč, I., Ďurkáč, J., Hollý, M., Jakubčová, K., Peržeľová, V., Mučaji, P., Švajdlenka, E., Sabol, F., Legáth, J., Belák, J., Smetana, K., Gál, J.P., “Plantago lanceolata L. water extract induces transition of fibroblasts into myofibroblasts and increases tensile strength of healing skin wounds,” Journal of Pharmacy and Pharmacology, 67: 117-125. 2015.
[49]	Ram, M., “Bilirubin modulated cytokines, growth factors and angiogenesis to improve cutaneous wound healing process in diabetic rats.” Internation Journal of Immunopharmacology, 3: 137-149. 2016.
[50]	Nayak, B.S., Sandiford, S. Maxwell, A., “Evaluation of the wound-healing activityof ethanolic of Morinda citrifolia L. Leaf,” Evidence Based Complementary and Alternative Medicine, 6: 351-356. 2009.
[51]	Shedoeva, A., Leavesley, D., Upton, Z. 1,2 Fan, C. “Wound healing and use of medicinal plants,” Evidence-Based Complementary and Alternative Medicine Volume 2019. Article ID 2684108, 30 pages 2019.
[52]	Heymann, F., Trotha, K., Preisinger, C., Lynen-Jansen, P., Roeth, A., Geiger, M.,Geisler L., Frank, A., Conze, J., Luedde, J., Trautwein, C., Binnebösel, M., Neumann, U., Tacke F., “Polypropylene mesh implantation for hernia repair causes myeloid cell-driven persistent inflammation,” JCI Insight, 4(2): e123862. 2019.
[53]	Muralidhar, A., Babu, S., Sankar, R., Reddanna, P., Latha, J., “Wound healing activity of flavonoid fraction isolated from the stem bark of Buteamono sperma (Lam) in albino wistar rats,” European Journal of Experimental Biology, 3(6): 1-6. 2013.
[54]	Okach, D.O., Nyunja, A.R.O., Opande, G., “Phytochemical screening of some wild plants from Lamiaceae and their role in traditional medicine in Uriri District - Kenya,” International Journal of Herbal Medicine, 1(5): 135-143. 2013.
[55]	Matthias, S., Sabine, W., “Oxidative stress in normal and impaired wound repair,” Pharmacological Research, 58 (2): 165-171. 2008.
[56]	Johansen, J. S., Harris, A. K., Rychly, D. J., “Oxidative stress and the use of antioxidants in diabetes: Linking basic science to clinical practice,” Cardiovascular Diabetology, 4: 5-12. 2005.
[57]	Asadi, S. Y., “Effect of green tea (Camellia sinensis) extract on healing process of surgical wounds in rat,” International Journal of Surgery, 11, 332-337. 2013.
[58]	Eroglu, A., Dogan, Z., Kanak, E. G., Atli, G., Canli, M., “Effects of heavy metals (Cd, Cu, Cr, Pb, Zn) on fish glutathione metabolism,” Environmental Science and Pollution Research, 22, 3229-3237. 2015.
[59]	Silveira, P. C. L. “Iontophoresis with gold nanoparticles improves mitochondrial activity and oxidative stress markers of burn wounds,” Materials Science and Engineering C, 44, 380-385. 2014.