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Applied Ecology and Environmental Sciences
ISSN (Print): 2328-3912 ISSN (Online): 2328-3920 Website: https://www.sciepub.com/journal/aees Editor-in-chief: Alejandro González Medina
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Issue 12, Volume 10
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Applied Ecology and Environmental Sciences. 2022, 10(12), 738-746
DOI: 10.12691/aees-10-12-6
Open AccessArticle

A Metagenomic Study of Gut Microflora of Rohu (Labeo rohita) from Wild Habitat and Aquaculture Settings

Shrihari Ashok Pingle1 and Abhay John Khandagle2,

1Department of Zoology, Prof. Ramkrishna More College of Arts, Commerce and Science, Pune, India

2Principal, Annasaheb Waghire Arts, Science and Commerce College, Otur, India

Pub. Date: December 18, 2022
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Cite this paper:
Shrihari Ashok Pingle and Abhay John Khandagle. A Metagenomic Study of Gut Microflora of Rohu (Labeo rohita) from Wild Habitat and Aquaculture Settings. Applied Ecology and Environmental Sciences. 2022; 10(12):738-746. doi: 10.12691/aees-10-12-6

Abstract

The present study aimed to explore and compare the gut microbiome of Labeo rohita (rohu) from wild and aquaculture setting through 16S rDNA sequencing. The amplification of the library of 16S rDNA V3-V4 hypervariable regions of the gut microbiome was carried out followed by sequencing using Illumina MiSeq. The analysis of sequencing data was carried out through the Quantitative Insights into Microbial Ecology (QIIME2) pipeline. Proteobacteria, Firmicutes, Cyanobacteria and Actinobacteria represented the bacterial phyla with large abundance. The abundance of Bacillus spp. and Clostridium spp. was high in cultured forms. Vagococcus spp. and Carnobacterium spp. were found to be abundant in wild forms. Cyanobium spp., and Pseudomonas spp. were abundant in both wild and cultured forms. In the present study, the gut of cultured forms of rohu shows a diverse microbiome than that of wild forms. The harbouring gut microbes may offer several benefits to the host fish such as carbohydrate digestion, antimicrobial activity, xenobiotic degradation and faster growth rate. The functional profiles from the 16s rRNA gene amplicon confirm that the cultured rohu hosts the bacteria that have a role in metabolism and compromised disease resistance. Absence of Bacillus spp. in wild form, and low abundance of Lactococcus spp. indicates the need of finding alternatives for probiotics. The information from the present study and its understanding can be used for exploring the role of the gut microbiome with regard to the growth, immunity and other physiological functions of the fish.

Keywords:
rohu fish gut metagenomics major carps aquaculture gut microbiome

Creative CommonsThis work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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