Mapping of Microbial Diversity of Gautala Reserve Forest in Aurangabad (District) (M.S.), India

Amrin Naimoddin Mirza^1, and Satish Sudhakarrao Patil¹

¹Dr. Babasaheb Ambedkar Marathwada University, Department of Environmental Science, Aurangabad

Cite this paper:
Amrin Naimoddin Mirza and Satish Sudhakarrao Patil. Mapping of Microbial Diversity of Gautala Reserve Forest in Aurangabad (District) (M.S.), India. Applied Ecology and Environmental Sciences. 2022; 10(5):303-310. doi: 10.12691/aees-10-5-6

Abstract

Microorganisms help in the fertility and endurance of soil. Additionally supporting the growth of several biological systems, soil and soil microorganisms fill in as the best mode for plant growth. Soil microbes are essential for recycling old plant material and decaying organic matter. In the upkeep of the Environment microbial diversity plays a dominant role. The current paper is focused on the seasonal variations in the microbial count, which are increased or decreased (Impact on counts) in the Gautala reserve forest. In the present study, soil samples from Gautala Reserve Forest were collected from 15 different sites during the rainy, winter, and summer seasons. The present study was attempted to cover the microbial diversity of the whole Gautala forest through covering maximum sampling spots. The average total microbial count (TMC) in the rainy season for plate 1 and plate 2 was 36.73 and 35.46 respectively and the final count was 36.4 × 10⁶. The average TMC in the winter season for plate 1 and plate 2 was 32.4 and 31.93 respectively and the final count was 32.46 × 10⁵. The average TMC in the summer season for plate 1 and plate 2 was 37.13 and 36.4 and the final count was 37.6 × 10⁴. The bacterial colony at sampling points 1, 4, and 7 exhibits presence of Gram-Negative Bacteria; whereas the rest of the sampling spots showed presence of Gram - Positive Bacteria. There were three types of Gram-negative and 12 were found to be Gram-positive bacteria with three genera i.e. Pseudomonas spp., Bacillus spp., and micrococcus spp. This paper discusses seasonal fluctuations in microbial counts and associated laboratory culture techniques and statistical analysis methods.

Keywords:
microbial diversity soil microbes Gautala reserve forest

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References:

[1]	Yardi D., Eco-sustainability Assessment of Jaikwadi Dam with reference to Bird Sanctuary, A thesis submitted to Dr. Babasaheb Ambedkar Marathwada University, Aurangabad. 2011.
[2]	Ghorade I. B., Eco sustainability assessment of Godavari river water for sustainable utilization. A thesis submitted to Dr. Babasaheb Ambedkar Marathwada University, Aurangabad. 2013.
[3]	Mirza Amrin Naimoddin and Patil Satish S., Seasonal Plant Diversity of Gautala Reserve Forest, District Aurangabad, Applied Ecology and Environmental Sciences, 9 (1), 92-10, January 2021.
[4]	Stine M. A. and Weil R. R., The relationship between soil quality and crop productivity across three tillage systems in south-central Honduras. American Journal of Alternative Agriculture, 17(1): 2-8. March 2002.
[5]	Braga R. M., Dourado M. N. and Araujo W. L., Microbial interactions: ecology in a molecular perspective, Braz J. Microbiol., 47, 86-98. October 2016.
[6]	Hongmei J., Aitchison J., Lacap D., Peerapornpisal, Y., Sompong, U. and Pointing, S., Community phylogenetic analysis of moderately thermophilic cyanobacterial mats from China, the Philippines and Thailand, Extremophiles, 9, 325-332. September 2005.
[7]	Gardi C., Montanarella L., Arrouays D., Bispo A., Lemanceau P., Jolivet C., Mulder C., Ranjard L., Rombke J., Rutgers M., and Menta C., Soil biodiversity monitoring in Europe: ongoing activities and challenges. European Journal Of Soil Sciences, Volume 60, Issue 5, 807-819. September 2009.
[8]	Gans J., Wolinsky M., and Dunbar J., Computational improvements reveal great bacterial diversity and high metal toxicity in soil. Science 309:1387-1390. August 2005.
[9]	Eichorst Stephanie A., Breznak John A., and Schmidt Thomas M., Isolation and Characterization of Soil Bacteria That Define Terriglobus gen. Nov., in the Phylum Acidobacteria, Applied and Environmental Microbiology, 73(8), 2708-2717. April 2007.
[10]	Potadar Vishnu R, and Satish S Patil., Sequestration and storage of carbon by trees in and around University campus of Aurangabad city in Maharashtra, India. International Research Journal of Engineering and Technology (IRJET). 04 (01), ISSN: 2395-0056, January 2017.
[11]	Kshirsagar Anil A., Pawar Sanjay M., Patil Nirmala P., and Mali Vasant P.: Diversity of medicinal plants in Gautala Sanctuary of Kannad, District Aurangabad (Ms) India. Bioscience Discovery, 3(3):355-361. November 2012.
[12]	Naik V. N., Flora of Marathwada. Vol. I and II, Amrut Prakashan, Aurangabad. 1998.
[13]	Mirza Amrin Naimoddin and Patil Satish S., Assessment of Seasonal Variation in Physicochemical Characteristics of the Soil at Gautala Reserve Forest (M.S), India. Curr World Environ, 15 (2), Pg. 289-303. June 2020.
[14]	Soman Reshma, Thomas Achamma, Sarma N. Minu, Varada Jagadeesh and Sankarshanan Mohan., Bacterial Diversity in Soil from Peripheral Areas of Thattekad Bird Sanctuary. Indian J. Sci. Res. 18 (2), 80-87. February 2018.
[15]	Waksman Selman A., A Method for Counting the Number of Fungi in the Soil. J Bacteriol. 7(3): 339-341. May 1922.
[16]	APHA, Standard Methods for the Examination of Water and Wastewater. Washington, D.C. 15th ed. 1980.
[17]	Tankeshwar Acharya, Pour Plate Method: Procedure, Uses, (Dis) Advantages, Microbe Online, (2021). https://microbeonline.com/pour-plate-method-principle-procedure-uses-dis-advantages/.
[18]	Taylor Raymond H., Allen Martin J. and Geldreich Edwin E., Standard plate count: A comparison of pour plate and spread plate methods, Journal (American Water Works Association), 75 (1), 35-37. January 1983.
[19]	Bertrand Jean Claude, Caumette Pierre, Lebaron Philippe, Matheron Robert, Normand Philippe and Telesphore Sime-Ngando., Environmental Microbiology: Fundamentals and Applications: Microbial Ecology. Presses Universitaires de Pau et des Pays de l’Adour, Springer, ISBN 978-94-017-9117-5. 2015.
[20]	Sharma Sonam, Kumar Vijay and Tripathi Ram Babu., Isolation of Phosphate Solubilizing Microorganism (PSMs) From Soil, J. Microbiol. Biotech. Res., 1 (2), 90-95. January 2011.
[21]	Biasi C., Rusalimova O., Meyer H., Kaiser C., Wanek, W., Barsukov P., Junger H. and Richter A., Temperature-dependent shift from labile to recalcitrant carbon sources of arctic heterotrophs, Mass Spectrom. 19, 1401-1408. May 2005.
[22]	Kavitha, A. Sudha, P. Ahila Devi and K. Kumaran., A Comparative Study on Forest Soil Microbial Diversity and Biomass in Nilgiri Biosphere of Southern India P.G. International Journal of Current Microbiology and Applied Sciences, 9 (9), ISSN: 2319-7706., August 2020.
[23]	Franca Luis, Ciro Sannino, Benedetta Turchetti, Pietro Buzzini, and Rosa Margesin., Seasonal and altitudinal changes of culturable bacterial and yeast diversity in Alpine forest soils. Extremophiles, 20, 855-873. November 2016.
[24]	Martinez J., Smith D., Steward G. F. and Azam. F., Variability in Ecto-hydrolytic enzyme activities of pelagic marine bacteria and its significance for substrate processing in the sea. Aqua. Microbial. Ecol., 10, 223-230. January 1996.
[25]	Davinic Marko, Fultz Lisa M., Acosta-Martinez Veronica, Calderon Francisco J., Cox Stephen B., Dowd Scot E., Allen Vivien G., Zak John C., and Moore-Kucera Jennifer., Pyrosequencing and mid-infrared spectroscopy reveal distinct aggregate stratification of soil bacterial communities and organic matter composition. Soil Biology & Biochemistry, Elsevier, 46, 63-72. December 2011.
[26]	Hattori T., Soil Aggregates in Microhabitats or Microorganisms, The Reports of the Institute for Agricultural Research, Tohoku University, 37, 23-36. January 1988.
[27]	Mummey, D., Holben, W., Six, J. and Stahl, P., Spatial stratification of soil bacterial populations in aggregates of diverse soils, Microbial Ecology, 51, 404-411. May 2006.
[28]	Mummey, D. L. and Stahl, P.D., Analysis of soil whole and inner-micro aggregate bacterial communities, Microbial Ecology, 48, 41-50. July 2004.
[29]	Banakar Shivakumar P., Thippeswamy B., Thirumalesh B. V., and Naveen Kumar K. J., Diversity of soil fungi in dry deciduous forest of Bhadra Wildlife Sanctuary, Western Ghats of southern India, Journal of Forestry Research, 23 (4), 631-64. November 2012.
[30]	Eisenhauer, N., Schulz, W., Scheu, S., and Jousset, A., Niche dimensionality links biodiversity and invasibility of microbial communities, Functional Ecology, 27, 282-288. February 2013.
[31]	Harpole W. S., and Tilman D., Grassland species loss resulting from reduced niche dimension, Nature 446, 791-793. April 2007.
[32]	Gupta V. V. S. R., and Germida J. J., Distribution of microbial biomass and its activity in different soil aggregate size classes as affected by cultivation, Soil Biology and Biochemistry 20, 777-786. January 1988.
[33]	Raaijmakers J., Paulitz T., Steinberg C., Alabouvette C., and Moenne-loccoz Y., The rhizosphere: a playground and battlefield for soilborne pathogens and beneficial microorganisms, Plant and Soil, 321, 341-361. January 2009.
[34]	Chen, D. et al., Isolation of Bacillus amyloliquefaciens S20 and its application in control of eggplant bacterial wilt, Journal of Environmental Management, 137, 120-127. May 2014.
[35]	Guo, J. H., et al., Biocontrol of tomato wilt by plant growth-promoting rhizobacteria, Biological Control, 29 (1), 66-72. January 2004.
[36]	Hu H. Q., Li X. S. and He H., Characterization of an antimicrobial material from a newly isolated Bacillus amyloliquefaciens from mangrove for biocontrol of Capsicum bacterial wilt, Biological Control, 54 (3), 359-365. January 2010.
[37]	Tan S. et al., Two Bacillus amyloliquefaciens strains isolated using the competitive tomato root enrichment method and their effects on suppressing Ralstonia solanacearum and promoting tomato plant growth, Crop Protection, 43, 134-140. January 2013.
[38]	Huang X., Zhang N., Yong X., Yang X. and Shen Q., Biocontrol of Rhizoctonia solani damping-off disease in cucumber with Bacillus pumilus SQR-N43, Microbiological Research 167(3), 135-143. March 2012.
[39]	Wang W., Li S., Li Z., Zhang J., Fan K., Tan G., Ai G., Lam S.M., Shui G., Yang Z., and et al., Harnessing the intracellular triacylglycerols for titer improvement of polyketides in Streptomyces. Nat. Biotechnol. 38: 76-83. January 2020.
[40]	Kramer C., and Gleixner G., Variable use of plant- and soil-derived carbon by microorganisms in agricultural soils, Soil Biol. Biochem., 38, 3267-3278. November 2006.