Applied Ecology and Environmental Sciences
ISSN (Print): 2328-3912 ISSN (Online): 2328-3920 Website: https://www.sciepub.com/journal/aees Editor-in-chief: Alejandro González Medina
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Applied Ecology and Environmental Sciences. 2021, 9(5), 575-579
DOI: 10.12691/aees-9-5-8
Open AccessArticle

Assessment of DNA Damage and Apoptosis in the Brain of Freshwater Fish Oreochromis mossambicus Exposed to Tannery Effluent Wastewater

N. Ganesan1, K. Samyappan1 and R. Saravanan1,

1Department of Zoology, Dr Ambedkar Government Arts College, Vyasarpadi, Chennai 600039, Tamil Nadu, India

Pub. Date: May 30, 2021

Cite this paper:
N. Ganesan, K. Samyappan and R. Saravanan. Assessment of DNA Damage and Apoptosis in the Brain of Freshwater Fish Oreochromis mossambicus Exposed to Tannery Effluent Wastewater. Applied Ecology and Environmental Sciences. 2021; 9(5):575-579. doi: 10.12691/aees-9-5-8

Abstract

The present study was designed to evaluate the effect of tannery effluent on the fingerlings of freshwater fish Oreochromis mossambicus. The fishes were maintained at 10% concentration of tannery effluent for a period of 30 days. Apoptosis was studied by activity of caspases. Caspase 3 and 9 activity showed significant alteration. DNA seperation by agarose gel electrophoresis shows difference in intensity of genomic DNA between 750-1000 bp in fishes exposed to tannery effluent. DNA damage was also confirmed by Comet assay which showed an increase in DNA damage and tail migration was indicated by increase in percentage of mean DNA comet tail length formation.

Keywords:
Oreochromis mossambicus Tannery effluent Brain Caspases 3 and 9 Genomic DNA Comet assay

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