Optimization Studies of Multistress Spirulina Isolated from Marine Water

T. Srinivasan^1, and S. Illanjiam¹

¹Department of Microbiology, Hindustan College of Arts and Science, Padur, Kelambakkam, Chennai - 603 103, Tamil Nadu, India

Cite this paper:
T. Srinivasan and S. Illanjiam. Optimization Studies of Multistress Spirulina Isolated from Marine Water. Applied Ecology and Environmental Sciences. 2021; 9(1):76-78. doi: 10.12691/aees-9-1-11

Abstract

The growth parameters of all the three isolated Spirulina strains such as optical density, cell population, specific growth rate and doubling time of isolated Spirulina strains were studied in Zarrouk’s medium. A. The SpirulinaS1 strain showed highest growth in terms of optical density value at 560nm (1.870), cell population (5.30 CFU ml-1), specific growth rate (0.038 and doubling time (18.23) on 30th day. The effects of temperature, pH, light intensity and salinity on growth of Spirulina strains (S1, S2, and S3) and reference strain (CAS10) were determined in Zarrouk’s medium. The optimum temperature for the growth of all Spirulina strains was found to be 35°C followed by 40°C and 30°C. The highest biomass (0.308 mg ml-1) were recorded in Spirulina-S1 strain at 35°C.

Keywords:
single cell protein Zarrouk’s medium optimum

This work is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

References:

[1]	Dabbah R. Algae as a food. Food Technology; 24: 659. 1970.
[2]	Sasson A. Micro Biotechnologies: Recent Developments and Prospects for developing Countries. BIOTEC Publication 1/2542, 1997; pp. 11-31. Place de Fontenoy, Paris. France. United Nations Educational, Scientific and Cultural Organization (UNESCO).
[3]	Tri-Panji and Suharyanto. Optimization media from low-cost nutrient sources for growing Spirulina platensis and carotenoid production. Menara Perkebunan, 69(1): 18- 28.2000.
[4]	Waslien, J. SCP as protein supplement. Critical Reviews in Food Science and Nutrition, 61: 77-80.1975.
[5]	Manojkumar, Jyoti K, Gajendra PS. Growth and biopigment accumulation of cyanobacterium Spirulina platensis at different light intensities and temperature. Brazilian Journal of Microbiology, 42: 1128-1135. 2011.
[6]	Hirsh, H., Coen, M.H., Mozer, M.C., Hasha, R. and Flanagan, J.L, “Room service, AI-style,” IEEE intelligent systems, 14 (2). 8-19. Jul.2002.