Facile Synthesis of Cobalt Oxide Nanoparticle for Biological Studies

Murugan Perachiselvi¹, J. Jenson Samraj¹, Muthiah Sakthi Bagavathy¹, E. Pushpalaksmi¹ and G. Annadurai^1,

¹Division of Nanoscience, Sri Paramakalyani Centre for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi 627 412, India

Cite this paper:
Murugan Perachiselvi, J. Jenson Samraj, Muthiah Sakthi Bagavathy, E. Pushpalaksmi and G. Annadurai. Facile Synthesis of Cobalt Oxide Nanoparticle for Biological Studies. Applied Ecology and Environmental Sciences. 2020; 8(5):269-272. doi: 10.12691/aees-8-5-12

Abstract

Currently Nanoparticles are utilized in various fields and have a different clinical application. In our research, we have developed the Cobalt oxide (Co₃O₄) NPs using the Sol-gel technique from the Cobalt chloride precursor. The synthesized nanoparticle was characterized by using X-Ray Diffraction (XRD), Scanning Electron Microscope (SEM), Dynamic Light Scattering (DLS), and Fourier Transform Infra-Red Spectroscopy (FTIR). The cytotoxicity studies were evaluated on the Vero cell line (African green monkey kidney cell line) by using MTT assay at 72 hrs. The Cell viability which was observed during the process depends upon the concentration and time exposure. Moreover, the In-vitro cytotoxic effects of Co₃O₄ NPs showed a better result at the concentration of 50 µg/100µl in Vero cell.

Keywords:
cobalt oxide NPs sol-gel technique MTT assay cytotoxicity vero cell line

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