Genotoxic Effect of Certain Pesticide Mixtures in CHO Cell Lines

B. Reddi Bhargavi¹, Rajanikanth Akurathi², Ravi Kumar Vellanki¹ and Jagadeeswara Reddy Kanala^2,

¹Department of Biotechnology, Vignan University, Vadlamudi, Guntur-522213, AP, India

²Sugen Life Sciences Pvt.Ltd. 4/86, S.V.Nagar, Perumallapalli, Tirupathi - 517502, India

Cite this paper:
B. Reddi Bhargavi, Rajanikanth Akurathi, Ravi Kumar Vellanki and Jagadeeswara Reddy Kanala. Genotoxic Effect of Certain Pesticide Mixtures in CHO Cell Lines. Applied Ecology and Environmental Sciences. 2020; 8(4):166-173. doi: 10.12691/aees-8-4-4

Abstract

The objective of the present study is to identify Chromosomal Aberrations (CA) and Micronuclei (MN) formation in Chinese hamster ovary (CHO) cell lines when treated with commercially available pesticide mixtures for 24 and 48hrs. CHO cell lines are exposed to pesticide mixtures (Deltamethrin 1% + Triazophos 35% EC (D+T) and Profenofos 40% EC + Cypermethrin 4% EC (P+C)) at a dose of 78.1 µg/ml to 5000 µg/ml with and without metabolic activation (S9). 10 µg/ml of Cyclophosphamide and Mitomycin C at a concentration of 0.4 - 0.8 µg/ml were used as the positive controls. Dose dependant increase of aberrations were recorded for both the pesticide mixtures. Chromosomal Aberrations were mainly observed in the form of chromatid gaps and breaks for both (D+T) and (P+C) treated cell lines of the CHO in comparison to negative control. An increased number of binucleated cells with micronuclei was found at the concentrations >625µg/ml of pesticide mixtures and is statistically significantly different from the control. Our findings suggest that both these pesticide mixtures at higher concentration are found to be a bit genotoxic.

Keywords:
Genotoxicity Chromosome aberrations Micronucleus CHO Chromatid gaps Emulsifiable concentrate (EC)

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