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M.C.Santiago-Santos,T.Ponce-Noyola,R.Olvera-Ramirez, J.Ortega-Lopez, R.O. Canizares-Villanueva, Extraction and purification of phycocyanin from Calothrix sp, Process Biochem. 39 (2004) 2047-2052.

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Article

Extraction and Separation of Phycocyanin from Spirulina using Aqueous Two-Phase Systems of Ionic Liquid and Salt

1The College of Agriculture and Biotechnology (CAB), Hexi University, Zhangye, P.R. China

2Kaiyuan Bio-tech Development Center, Hexi University, Zhangye, P.R. China


Journal of Food and Nutrition Research. 2015, Vol. 3 No. 1, 15-19
DOI: 10.12691/jfnr-3-1-3
Copyright © 2014 Science and Education Publishing

Cite this paper:
Xifeng Zhang, Fenqin Zhang, Guanghong Luo, Shenghui Yang, Danxia Wang. Extraction and Separation of Phycocyanin from Spirulina using Aqueous Two-Phase Systems of Ionic Liquid and Salt. Journal of Food and Nutrition Research. 2015; 3(1):15-19. doi: 10.12691/jfnr-3-1-3.

Correspondence to: Guanghong  Luo, Kaiyuan Bio-tech Development Center, Hexi University, Zhangye, P.R. China. Email: 464690924@qq.com

Abstract

To explore a new and simple rapid extraction and purification technique for phycocyanin, an ionic liquids(ILs)-based aqueous two-phase system(ATPS) was developed for the purification of phycocyanin from Spirulina extracts. Effects of various process parameters such as the concentrations of [Bmim]Cl, the concentrations of KH2PO4, the concentrations of crude phycocyanin, the system pH and the temperature on partitioning of phycocyanin were evaluated. The obtained data indicated that phycocyanin was preferentially partitioned into the ILs-rich phase and the ATPS composed of 23% (w/w) [Bmim]Cland 29% (w/w) KH2PO4 at 30°C and pH 7.0 showed good selectivity on phycocyanin. Under the optimum conditions, phycocyanin with a purity of 3.98 and yield of about 90.23 % was obtained. Therefore, ILs-based ATPS was an effective method for partitioning and recovery of phycocyanin from Spirulina extracts.

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