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Jahic M, Gustavsson M, Jansen A, Martinelle M, Enfors S: Analysis and control of proteolysis of a fusion protein in Pichia pastoris fed-batch processes. J Biotechnol 2003, 102(1): 45-53.

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Article

Control of the Ratio of Inducer to Cell Concentration to Enhance the Phytase Production in Recombinant Pichiapastoris

1College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China

2College of Sciences, Nanjing Tech University, Nanjing, China


Journal of Food and Nutrition Research. 2014, Vol. 2 No. 12, 980-984
DOI: 10.12691/jfnr-2-12-19
Copyright © 2014 Science and Education Publishing

Cite this paper:
Housheng Hong, Zhaosheng Min, Huiming Guo. Control of the Ratio of Inducer to Cell Concentration to Enhance the Phytase Production in Recombinant Pichiapastoris. Journal of Food and Nutrition Research. 2014; 2(12):980-984. doi: 10.12691/jfnr-2-12-19.

Correspondence to: Housheng  Hong, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China. Email: hhs@njtech.edu.cn

Abstract

Phytase production by Pichiapastoris was used as a case to study the mechanism and strategy for optimization of heterologous protein production. It was found that the ratio of inducer-methanol to cell concentration had a significant influence on phytase production. In this case, we found that the optimum initial cell concentration and methanol concentration were 85 g/L and 10 g/L, respectively. During induction period, an easy-to-control methanol feeding method was proposed according to the optimal ratio of methanol to cell concentration at a range of 0.063 -0.132 g/g, and phytase activity and productivity reached 53984 U/mL and 529.25 U/(mL·h), respectively. The method for optimization of phytase production through controlling the optimal ratio maybe provide an alternative idea to enhance other heterologous protein production with P. pastoris.

Keywords