Meihong Xu^{1, 2}, Rui Liang^{1, 2}, Qianying Guo^{1, 2}, Shuangjia Wang^{1, 2}, Ming Zhao^{1, 2}, Zhaofeng Zhang^{1, 2}, Junbo Wang^{1, 2}, Yong Li^{1, 2,}

To assess the long-term toxicity and carcinogenicity of dietary nucleotides (NTs), Sprague-Dawley rats were administered the NTs for life-through with the dose of 0, 0.01%, 0.04%, 0.16% and 0.64%. With the exception of several groups, the results showed no significant differences in weight gain, clinical symptoms data, blood indicators, and Non-neoplastic lesions frequencies. NTs have dramatically increased the food consumption of both sexes (P<0.05). Meanwhile, NTs decreased spontaneous tumor incidence of both sexes, notably the male rats (P<0.05). The occurrence of all malignant and mammary tumors in NTs-treated females was lower than those in controls (P<0.05). In comparison with the control group, the incidence of death from malignant and systemic tumors were obviously decreased in the NTs-treated groups (P<0.01). Moreover, our results showed that a higher percentage of rats in the NTs-treated groups died of non-neoplastic lesions compared with control. In conclusion, dietary nucleotides are not toxic or carcinogenic in rats up to 0.64% for all the life, and 0.64% was an unobservable effect dose.

dietary nucleotides, safety, lifelong, Spontaneous Tumor Incidence, Sprague-Dawley rat