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Ye XY, Ng TB, Rao PF, “A Bowman-Birk-type trypsin-chymotrypsin inhibitor from broad beans”. Biochemical and Biophysical Research Communications 289, 91-96, 2001.

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Article

Purification and Characterization of Bowman-Birk Trypsin Inhibitor from Soybean

1Institute of Food Science and Engineering, Jilin Agricultural University, Changchun, China

2Institute of Animal Science and Technology, Jilin Agricultural University, Changchun, China


Journal of Food and Nutrition Research. 2014, Vol. 2 No. 9, 546-550
DOI: 10.12691/jfnr-2-9-3
Copyright © 2014 Science and Education Publishing

Cite this paper:
Chunmei Gu, Xinxiu Song, Linlin Zhao, Shu Pan, Guixin Qin. Purification and Characterization of Bowman-Birk Trypsin Inhibitor from Soybean. Journal of Food and Nutrition Research. 2014; 2(9):546-550. doi: 10.12691/jfnr-2-9-3.

Correspondence to: Guixin  Qin, Institute of Animal Science and Technology, Jilin Agricultural University, Changchun, China. Email: qgx@jlau.edu.cn

Abstract

In this paper, crude extract of a Bowman-Birk trypsin inhibitor from soybean meal was firstly isolated by a combination of , thermal denaturation, isoelectric precipitation and acetone precipitation. Then this extract was purified by DE-52 ion exchange and affinity chromatography. The results showed that soybean Bowman-Birk trypsin inhibitor (SBBI) was purified to 50.07-fold with trypsin activity of 822.31 U·mg-1. The purified SBBI gave a single protein band in SDS-PAGE electrophoresis. The accurate molecular mass of this inhibitor was determined to be 8837.46Da by MALDI-TOF. N-terminal sequence showed high homology with other serine proteinase inhibitors belonging to the Leguminosae family.

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