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Khalaf A.N, Shakya A.K, Al-othman A, El-Agbar Z, Farah H, “Antioxidant Activity of Some Common Plants”, Turk J Biol, 32. 51-55. 2008.

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Article

Determination of Antioxidant and Antimicrobial Activities of Medically Important Mushrooms Using Different Solvents and Chemical Composition via GC/MS Analyses

1Department Molecular Biology and Genetics/Biochemistry, Hitit University, Corum, Turkey

2Department Molecular Biology and Genetics/ Molecular Microbiology, Hitit University, Corum, Turkey

3Department Chemistry, Hitit University, Corum, Turkey


Journal of Food and Nutrition Research. 2014, Vol. 2 No. 8, 429-434
DOI: 10.12691/jfnr-2-8-1
Copyright © 2014 Science and Education Publishing

Cite this paper:
Emre Avcı, Gulcin Alp Avcı, Dursun Ali Kose. Determination of Antioxidant and Antimicrobial Activities of Medically Important Mushrooms Using Different Solvents and Chemical Composition via GC/MS Analyses. Journal of Food and Nutrition Research. 2014; 2(8):429-434. doi: 10.12691/jfnr-2-8-1.

Correspondence to: Gulcin  Alp Avcı, Department Molecular Biology and Genetics/ Molecular Microbiology, Hitit University, Corum, Turkey. Email: gulcinalp@hitit.edu.tr

Abstract

The aim of present work was the determination of antioxidant activities of the ethanol extract from three mushrooms (Ganoderma lucidium, Lentinula edodes and Coprinus micaceus) by using DPPH radical scavenging and total antioxidant status assays. Additionally, in this study, GC/MS analyses and antimicrobial activity assay of three mushrooms were made. Ethanol, chloroform and distilled water used as solvent for preparation of mushrooms extract. Radical scavenging activities and total antioxidant status of three mushrooms extracted in ethanol were measured via spectrophotometric methods. In addition, for chromatographic analysis GC/MS were used. At the same time, diphenic acid and bis(2-ethylhexyl)pthalate derivatives were found the highest in contents of all extracts with GC/MS. The determination of antimicrobial activity of mushrooms extracts against Pseudomonas aeroginosa (ATCC 27853), Escherichia coli (ATCC 25922), Enterococcus faecalis (ATCC 29212), Staphylococcus aureus (ATCC 25923) and Candida albicans (ATCC 10231) was investigated by disc and agar-well diffusion methods. As result, radical scavenging activities and total antioxidant status values of G. lucidium extract were found higher than those of both L. edodes and C. micaceus. All mushrooms used in this study were found to have antimicrobial effects at a variety of degrees against microorganisms tested. Moreover, all extracts together with solvents were analyzed, ethanol and chloroform extracts were found to be more effective for G. lucidum and C. micaceus, and L. edodes than other solvents, respectively.

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