Non-aqueous, lipophilic metabolites in the meat, fat, and eggs of a 4-year-old female Siberian sturgeon (Acipenser baerii) were analyzed. A total of 616 lipid molecular species were detected, and 373 were used for statistical analysis. The primary common metabolites among meat, fat, and egg were lysophosphatidylcholine (LPC) with fatty acid moieties of 18:1, 18:2, 20:5, and 22:6, and di-/tri-acylglycerolipids with fatty acid moieties of 16:0, 18:1, 18:2, and a few other unsaturated ones. The meat, fat, and egg tissues were separated according to their lipid composition by principal component analysis, and tissue-specific lipid biomarkers were detected by linear discriminant analysis. Representative biomarkers were lysoglycerophosphoserine with 22:6 fatty acid for meat, platelet-activating factor with 20:5 fatty acid for fat, and glycerophosphoethanolamine for eggs. The abundant LPC in this lipidomic study may be related to choline found in earlier aqueous metabolomic analysis using the same sturgeon. The coupling of lipidomics and aqueous metabolomics is thus suggested to be insightful and will help in the development of aquaculture for endangered sturgeons.

sturgeon, lipidome, lipidomics, glycerolipids, glycerophospholipids, choline, fatty acids, EPA, DHA