Jiayuan Zhu¹, Tao Zheng¹, Yawei Liu¹, Maijiao Peng¹, Zhoujin Tan^1,

Human health was affected strongly by diet, due to the composition and structure of intestinal microbiota mainly. This study aimed to characterize the intestinal mucosal bacteria of diarrheal mice caused by high-fat and high-protein diet. Ten specific pathogen free (SPF) Kunming male mice were chosen and randomly divided into control group and model group. The control group (FCM) mice were general feed diet. The model group (FMM) mice were high-fat and high-protein diet. After successful diarrhea, the mice's intestinal mucosa was collected for microbial analysis. Results showed that the number of OTUs (Operational Taxonomic Unit), the Chao 1 index and Shannon index (p<0.05) increased in high-fat and high-protein diet group. Compared to FCM, taxonomic composition indicated Bacteroidetes, Verrucomicrobia, Actinobacteria, Streptophyta, Deferribacteres were significantly increased and the Firmicutes was decreased in FMM. The genus of Helicobacter, Afipia., Methylobacterium, Pseudomonas, Clostridium, Phocaeicola and Faecalibaculum were higher in FMM, while Bradyrhizobium and Lactobacillus were lower than FCM. Bacterial species such as Helicobacter typhlonius, Methylobacterium sp., Afipia genosp. 1, Bacteroides vulgatus ATCC 8482, Faecalibaculum rodentium and Pseudomonas in FMM were significantly increased than FCM, but Lactobacillus johnsonii and Lactobacillus reuteri were decreased in FMM. To sum up, High-fat and high-protein diet results in intestinal mucosal microbiota dysbiosis, increases the number of conditionally pathogenic bacteria, reduces the number of beneficial bacteria, which trigger for diarrhea and may affect the Protein and fat catabolism and metabolism.

high-fat and high-protein diet, diarrhea, intestinal mucosa, intestinal micorbiota