Indra Wibowo^1,, Fauzi Ramadhani Nasution¹, Intan Taufik¹, Roya Suffah Zain¹, Nissa Marlinda¹, Nuruliawaty Utami², Putri Yunitha Wardiny¹, Ramadhani Eka Putra¹, Marselina Irasonia Tan¹, Sony Heru Sumarsono¹

One candidate for anti-inflammatory agents, polyphenol-rich Indonesian propolis, has been rarely studied. This study was conducted to confirm the presence of its anti-inflammatory activity. Zebrafish larvae, as a model, were divided into four groups consisting of a control group, a lipopolysaccharide (LPS)-treated group, an LPS-treated group followed by treatment in propolis solution for 24 hours, and a propolis-treated group. Myeloid leukocytes migrating into the intestine and intestinal goblet cells were counted. The expression level of pro-inflammatory (tnf-α, il-1β, il-8, and il-6) and anti-inflammatory (il-10) cytokine genes were determined using quantitative reverse transcription polymerase chain reaction (RT-PCR). It was shown that Indonesian propolis administration to LPS-induced zebrafish larvae resulted in reduced myeloid leukocytes in the intestine, increased intestinal goblet cells, and decreased the expression level of tnf-α (P<0.05). Overall, these results suggest that Indonesian propolis could be a potential agent to protect against inflammatory damage.

indonesian propolis, inflammation, cytokine, lipopolysaccharide, zebrafish