Irene Ahou kouadio^{1, 2,}, Mi-Kyung Lee¹, Kap-Hoon Han^{1, 3}, Jae-Hyuk Yu¹

The berries of Solanumindicum L. are known to be an appreciable source of antioxidants. In this study, the effect of the green berries’ ethanolic extract on growth, RNA concentration representing the biomass and the percentage of reduced Alamar Blue indicating cell viability of Aspergillusnidulans, Aspergillusfumigatus and Aspergillusflavus was evaluated. It was noted a reduction of growth with the increasing of the extract content in the medium. Indeed, a radial growth of 90 mm was observed on the medium without extract for the three species after 7 days of incubation, while on the medium at 1 % of the berries extract, the radial growths were 0 mm for A. nidulansand A. fumigatus and 14.67 mm for A. flavus. In addition to the absence of growth of Aspergillusnidulans and Aspergillusfumigatus observed at 1 % of berries extract, an absence of their conidia germination was observed. It was noted also that the mean of RNA concentration observed in the medium without extract was about 285 times that observed in the medium at 1 % of berries extract for A. nidulans. For A. fumigatusand A. flavus, this mean of RNA concentrations observed in the medium without extract were respectively about 73 and 57 times that observed in the medium at 1 % of berries extract. The RNA bands of the three species tested observed after the electrophoresis seem to be degraded also in the medium at 1 % of berries extract. At this concentration, the percentages of surviving cells were less than 10 % for the three species tested, while that observed in the medium without the berries extract was 100 %.Thus, Solanumindicum green berries extract exhibit a real inhibitory effect against fungi with an ability of killing them.

Solanumindicum L., green berries, Aspergillus, biomass, cell viability