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Article

Prevalence and Antibiotic Resistance of Escherichia coli O157:H7 Isolated from Raw Meat Samples of Ruminants and Poultry

1Department of Food Hygiene, Karaj Branch, Islamic Azad University, Karaj, Iran


Journal of Food and Nutrition Research. 2018, Vol. 6 No. 2, 96-102
DOI: 10.12691/jfnr-6-2-5
Copyright © 2018 Science and Education Publishing

Cite this paper:
Zohreh Mashak. Prevalence and Antibiotic Resistance of Escherichia coli O157:H7 Isolated from Raw Meat Samples of Ruminants and Poultry. Journal of Food and Nutrition Research. 2018; 6(2):96-102. doi: 10.12691/jfnr-6-2-5.

Correspondence to: Zohreh  Mashak, Department of Food Hygiene, Karaj Branch, Islamic Azad University, Karaj, Iran. Email: Mashak@kiau.ac.ir

Abstract

Escherichia coli O157:H7 is one of the most dangerous zoonotic pathogens of meat. The present investigation was done to study the prevalence and antibiotic resistance of E. coli O157:H7 strains isolated from raw meat samples. A total of 458 meat samples were collected. Samples were cultured using what? After which sorbitol negative isolates were analyzed for rfbO157 and flicH7 genes. Thirty-six out of 458 meat samples were positive for sorbitol negative strains of E. coli (7.86%). All of the sorbitol negative strains were also positive for rfbO157 and flicH7 genes. Prevalence of E. coli O157:H7 strains were 7.86%. The prevalence of E. coli O157:H7 in chicken meat was higher than the other samples (16.25%). The genes that encode resistance to ampicillin (CITM) (100%), gentamicin (aac(3)-IV) (94.44%) and tetracycline (tetA) (61.11%) had the highest prevalence. Escherichia coli O157:H7 strains from raw meat samples from ruminants and poultry had the highest resistance to ampicillin (100%), tetracycline (83.33%) and gentamicin (83.33%) respectively. Strains of antibiotic resistant E. coli 0157:H7 found in this present study are of public health importance.

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